Natural curvature for manifest T-duality

We reformulate the manifestly T-dual description of the massless sector of the closed bosonic string, directly from the geometry associated with the (left and right) affine Lie algebra of the coset space Poincare/Lorentz. This construction initially doubles not only the (spacetime) coordinates for translations but also those for Lorentz transformations (and their dual). As a result, the Lorentz connection couples directly to the string (as does the vielbein), rather than being introduced ad hoc to the covariant derivative as previously. This not only reproduces the old definition of T-dual torsion, but automatically gives a general, covariant definition of T-dual curvature (but still with some undetermined connections).Comment: Minor changes in notations (see e.g. eq.(7), eq.(8)). Some typos corrected: e.g factor "i" in equations (11) and (12). New references adde

Segmentation of Speech and Humming in Vocal Input

Non-verbal vocal interaction (NVVI) is an interaction method in which sounds other than speech produced by a human are used, such as humming. NVVI complements traditional speech recognition systems with continuous control. In order to combine the two approaches (e.g. "volume up, mmm") it is necessary to perform a speech/NVVI segmentation of the input sound signal. This paper presents two novel methods of speech and humming segmentation. The first method is based on classification of MFCC and RMS parameters using a neural network (MFCC method), while the other method computes volume changes in the signal (IAC method). The two methods are compared using a corpus collected from 13 speakers. The results indicate that the MFCC method outperforms IAC in terms of accuracy, precision, and recall

Mott Children's Health Center Eatfit Summer Camp II

The purpose of this study was to design an evaulation tool for EatFit Summer Camp II held at Mott Children's Health Center. This camp is in need of a formal evaluation tool to be utilized by participants thirteen to sixteen years of age. The variables that will be concentrated on are self-efficacy and environment. The final MCHC questionnaire is comprised from questions from three existing evaluation tools. Two of the tools were utilized by children/adolescents. The other questionnaire was utilized by adults; however, questions regarding environment are relevant to population being serviced.Master'sSchool of Health Professions and Studies: Health EducationUniversity of Michiganhttp://deepblue.lib.umich.edu/bitstream/2027.42/117736/1/Polacek.pd

Ribosome-Associated ncRNAs (rancRNAs) Adjust Translation and Shape Proteomes

The regulation of protein synthesis is of extreme importance for cell survival in challenging environmental conditions. Modulating gene expression at the level of translation allows a swift and low‐energy‐cost response to external stimuli. In the last decade, an emerging class of regulatory ncRNAs, namely ribosome‐associated non‐coding RNAs (rancRNAs), has been discovered. These rancRNAs have proven to be efficient players in the regulation of translation as a first wave of stress adaptation by directly targeting the ribosome, the central enzyme of protein production. This underlying principle appears to be highly conserved, since rancRNAs are present in all three domains of life. Here, we review the major findings and mechanistic peculiarities of rancRNAs, a class of transcripts that is providing new and broader perspectives on the complexity of the ribosome and translation regulation

Revealing stable processing products from ribosome-associated small RNAs by deep-sequencing data analysis

The exploration of the non-protein-coding RNA (ncRNA) transcriptome is currently focused on profiling of microRNA expression and detection of novel ncRNA transcription units. However, recent studies suggest that RNA processing can be a multi-layer process leading to the generation of ncRNAs of diverse functions from a single primary transcript. Up to date no methodology has been presented to distinguish stable functional RNA species from rapidly degraded side products of nucleases. Thus the correct assessment of widespread RNA processing events is one of the major obstacles in transcriptome research. Here, we present a novel automated computational pipeline, named APART, providing a complete workflow for the reliable detection of RNA processing products from next-generation-sequencing data. The major features include efficient handling of non-unique reads, detection of novel stable ncRNA transcripts and processing products and annotation of known transcripts based on multiple sources of information. To disclose the potential of APART, we have analyzed a cDNA library derived from small ribosome-associated RNAs in Saccharomyces cerevisiae. By employing the APART pipeline, we were able to detect and confirm by independent experimental methods multiple novel stable RNA molecules differentially processed from well known ncRNAs, like rRNAs, tRNAs or snoRNAs, in a stress-dependent manne

Effects of Glycemic Load and Exercise on Overweight/Obesity in College Students

We sought to assess the effect of glycemic load consumption and exercise in healthy college students. Participants (N=10) were screened on physiological measures then randomly assigned to experimental and control groups. Both groups participated in the walking program. Those in the experimental group were given the ADA diet exchange list modified to low glycemic load. At the end of 12 weeks, participants were reassessed. Members of the experimental group saw significant changes in total cholesterol and blood glucose. Changes in body mass index and waist circumference were not significant but some changes were noted. The glycemic load does have a positive effect on blood chemistries and physiological measures

tRNA Synthetases Are Recruited to Yeast Ribosomes by rRNA Expansion Segment 7L but Do Not Require Association for Functionality

Protein biosynthesis is essential for any organism, yet how this process is regulated is not fully understood at the molecular level. During evolution, ribosomal RNA expanded in specific regions, referred to as rRNA expansion segments (ES). First functional roles of these expansions have only recently been discovered. Here we address the role of ES7La located in the large ribosomal subunit for factor recruitment to the yeast ribosome and the potential consequences for translation. Truncation of ES7La has only minor effects on ribosome biogenesis, translation efficiency and cell doubling. Using yeast rRNA deletion strains coupled with ribosome-specific mass spectrometry we analyzed the interactome of ribosomes lacking ES7La. Three aminoacyl-tRNA synthetases showed reduced ribosome association. Synthetase activities however remained unaltered suggesting that the pool of aminoacylated tRNAs is unaffected by the ES deletion. These results demonstrated that aminoacylation activities of tRNA synthetases per se do not rely on ribosome association. These findings suggest a role of ribosome-associated aminoacyl-tRNA synthetase beyond their core enzymatic functions