The SAR11 Group of Alpha-Proteobacteria Is Not Related to the Origin of Mitochondria

Although free living, members of the successful SAR11 group of marine alpha-proteobacteria contain a very small and A+T rich genome, two features that are typical of mitochondria and related obligate intracellular parasites such as the Rickettsiales. Previous phylogenetic analyses have suggested that Candidatus Pelagibacter ubique, the first cultured member of this group, is related to the Rickettsiales+mitochondria clade whereas others disagree with this conclusion. In order to determine the evolutionary position of the SAR11 group and its relationship to the origin of mitochondria, we have performed phylogenetic analyses on the concatenation of 24 proteins from 5 mitochondria and 71 proteobacteria. Our results support that SAR11 group is not the sistergroup of the Rickettsiales+mitochondria clade and confirm that the position of this group in the alpha-proteobacterial tree is strongly affected by tree reconstruction artefacts due to compositional bias. As a consequence, genome reduction and bias toward a high A+T content may have evolved independently in the SAR11 species, which points to a different direction in the quest for the closest relatives to mitochondria and Rickettsiales. In addition, our analyses raise doubts about the monophyly of the newly proposed Pelagibacteraceae family

Going Deeper: Metagenome of a Hadopelagic Microbial Community

The paucity of sequence data from pelagic deep-ocean microbial assemblages has severely restricted molecular exploration of the largest biome on Earth. In this study, an analysis is presented of a large-scale 454-pyrosequencing metagenomic dataset from a hadopelagic environment from 6,000 m depth within the Puerto Rico Trench (PRT). A total of 145 Mbp of assembled sequence data was generated and compared to two pelagic deep ocean metagenomes and two representative surface seawater datasets from the Sargasso Sea. In a number of instances, all three deep metagenomes displayed similar trends, but were most magnified in the PRT, including enrichment in functions for two-component signal transduction mechanisms and transcriptional regulation. Overrepresented transporters in the PRT metagenome included outer membrane porins, diverse cation transporters, and di- and tri-carboxylate transporters that matched well with the prevailing catabolic processes such as butanoate, glyoxylate and dicarboxylate metabolism. A surprisingly high abundance of sulfatases for the degradation of sulfated polysaccharides were also present in the PRT. The most dramatic adaptational feature of the PRT microbes appears to be heavy metal resistance, as reflected in the large numbers of transporters present for their removal. As a complement to the metagenome approach, single-cell genomic techniques were utilized to generate partial whole-genome sequence data from four uncultivated cells from members of the dominant phyla within the PRT, Alphaproteobacteria, Gammaproteobacteria, Bacteroidetes and Planctomycetes. The single-cell sequence data provided genomic context for many of the highly abundant functional attributes identified from the PRT metagenome, as well as recruiting heavily the PRT metagenomic sequence data compared to 172 available reference marine genomes. Through these multifaceted sequence approaches, new insights have been provided into the unique functional attributes present in microbes residing in a deeper layer of the ocean far removed from the more productive sun-drenched zones above

Self-similarity of low-frequency earthquakes

Low-frequency earthquakes are a particular class of slow earthquakes that provide a unique source of information on the physical processes along a subduction zone during the preparation of large earthquakes. Despite increasing detection of these events in recent years, their source mechanisms are still poorly characterised, and the relation between their magnitude and size remains controversial. Here, we present the source characterisation of more than 10,000 low-frequency earthquakes that occurred during tremor sequences in 2012–2016 along the Nankai subduction zone in western Shikoku, Japan. We show that the scaling of seismic moment versus corner frequency for these events is compatible with an inverse of the cube law, as widely observed for regular earthquakes. Their radiation, however, appears depleted in high-frequency content when compared to regular earthquakes. The displacement spectrum decays beyond the corner frequency with an omega-cube power law. Our result is consistent with shear rupture as the source mechanism for low-frequency earthquakes, and suggests a self-similar rupture process and constant stress drop. When investigating the dependence of the stress drop value on the rupture speed, we found that low-frequency earthquakes might propagate at lower rupture velocity than regular earthquakes, releasing smaller stress drop

Automatic Detection and Location of Microseismic Events from Sparse Network and Its Application to Post-mining Monitoring

International audienceIn post-mining regions with seismic hazard, timely decision making for risk management faces the challenge of quick and reliable detection and location of seismic events. As a response to the increasing density of monitoring stations, generating large volumes of seismic data, automatic, full waveform-based methods have been developed in recent years in global seismology. Such methods often cannot be directly applied to post-mining monitoring with a limited station coverage, as it is the case when temporarily networks are installed as an emergency response. In this paper we propose a new methodology that bridges this gap and enables the application of a full waveform, backprojection based method (BackTrackBB) to data of sparse network. The methodology was successfully tested on an abandoned and flooded underground coalmine in South-eastern France. Steps preceding BackTrackBB application were implemented in order to remove the coherent noise that otherwise results in numerous false detections. First results indicate that seismic activity in the study area is controlled by water level variation within former room-and-pillar mine works and fault segments (re)activation below them

Statistical Analysis of the Preparatory Phase of the Mw8.1 Iquique Earthquake, Chile

The 2014 Iquique seismic crisis in Chile, culminating with a Mw 8.1 earthquake on 1 April, highlights a complex unlocking of the Northern Chilean subduction that has been considered a seismic gap since 1877. During the year preceding this event, at least three clusters of seismic activity have been reported: in July 2013 and January and March 2014. Recent studies have proposed large-scale slab deformation as a potential trigger for the megathrust earthquake, and these clusters possibly indicate aseismic slip transients accompanying the progressive destabilization of the plate contact. However, no evidence of gradual unlocking of the interface or transient deformation has yet been found in the seismicity rate. To address this question, we develop a dense earthquake catalog covering 15 months preceding the mainshock and derived from the continuous waveform data set recorded by the Integrated Plate Boundary Observatory Chile (IPOC) and Iquique Local Network (ILN) networks. After declustering the seismicity, a space-time analysis highlights a large-scale acceleration of the seismicity along the interface accompanied by a deceleration of intermediate-depth earthquakes. We demonstrate the existence of a seismic quiescence downdip of the mainshock rupture before the July 2013 cluster. We propose that this seismic quiescence potentially highlights fluid circulation and/or aseismic motion along upper-plate crustal fault(s)

Migration of seismic activity associated with phreatic eruption at Merapi volcano, Indonesia

Phreatic activity of Merapi started after nearly 2 years of quiescence following the October-November 2010 eruption which was the largest in more than 100 years. A dozen eruptions identified by visual andior seismic observations took place between August 2012 and April 2014. We present in this work the results of a detailed analysis of the April 20, 2014 phreatic eruption. We attempted to reconstruct the eruptive process, which lasted for over 30 min. To this end, we determined the wavefield composition by polarization analysis, located high-frequency earthquakes occurring in the initial part of the eruption process and then determined the seismic source migration of low-frequency part of the tremor-like signal [0.3-3 Hz] over time. Source depth of low-frequency signal was obtained by comparing the slowness vector calculated using 3 stations of the seismic antenna with a slowness vector model obtained by ray tracing in the structure, taking into account the topography and a 1D velocity model obtained by spatial auto-correlation analysis. The results allow to distinguish 3 different phases: 1) High-frequency transients interpreted as the result of a sudden decompression caused by the transition of the volcanic fluid to a gaseous phase that occurred approximately 1.5 km deep. This decompression process in the hydrothermal system generated a migration of the low-frequency seismic source from 900 m to 1800 m above sea level: 2) A second decompression process revealed by high-frequency micro-seismicity and associated to the migration of the low-frequency tremor source which is marked first by a descent phase, followed by a sharp ascent until reaching the surface. The evolution of the back-azimuth during the migration process indicates a slight inclination of the conduit, presumably in the orientation of the dome fracture, in the NW-SE direction. This direction is consistent with the alignment of regional tectonic structures and with the directivity of eruption deposits. 3) The seismic source then remains positioned at the altitude of the dome for over 10 min. This phase probably corresponds to the ash emission process. The average migration speed of the low-frequency seismic source from the starting eruptive process to ash emission is about 5 m/s