Perancangan Sistem Dan Denah Bazaar Dengan Memanfaatkan Canvas HTML5 Berbasis Website Dan Android

At present, almost every organizers holds an attractive bazaar due to the growing number of enthusiasts who want to join bazaar. Bazaar can be an alternative place to sell new product and introduce the local product to the general society. Booking booth process in bazaar still using manual system, which tenant fills the order form and then the form is scanned to be sent to the organizer of the event via e-email. Once the booking is received, event organizer has to check and make a registration list of tenants whom will join the bazaar. Therefore there is a need to have an information system to help both eventorganizer and tenant to make a better booking process. Aplication Bazaar based on Android and website intends to help user do all booking process efficiently.Application based on mobile assist tenant in making reservation from fill form until payment order. It helps tenant to search for information about the bazaar which will be held in accordance with bazaar categories of interest and can follow event, so tenant will be easier to get bazaar information. In addition, application based on website helps event organizer to manage the whole process from booking to the payment which received from tenant order, then event organizer can edit event map in order to facilitate tenant when choose a booth at the bazaar.The test results which obatained have two modules, Mobile Application based on Android for tenant and application website for event organizer. Mobile Application can make a booking in bazaar, can get a notification, follow the event, displaying order data. Application website for the event organizer can manage the whole order and payment from tenant, and can edit map event to help tenant make an order. From the test of mobile application that have been done, 50% of respondents rate the app is overall good, and 42% of respondent rate the app overall very good. For Website Application, event organizer has satified with the existing features on the website

Lrp4 domains differentially regulate limb/brain development and synaptic plasticity.

Apolipoprotein E (ApoE) genotype is the strongest predictor of Alzheimer's Disease (AD) risk. ApoE is a cholesterol transport protein that binds to members of the Low-Density Lipoprotein (LDL) Receptor family, which includes LDL Receptor Related Protein 4 (Lrp4). Lrp4, together with one of its ligands Agrin and its co-receptors Muscle Specific Kinase (MuSK) and Amyloid Precursor Protein (APP), regulates neuromuscular junction (NMJ) formation. All four proteins are also expressed in the adult brain, and APP, MuSK, and Agrin are required for normal synapse function in the CNS. Here, we show that Lrp4 is also required for normal hippocampal plasticity. In contrast to the closely related Lrp8/Apoer2, the intracellular domain of Lrp4 does not appear to be necessary for normal expression and maintenance of long-term potentiation at central synapses or for the formation and maintenance of peripheral NMJs. However, it does play a role in limb development

Reelin regulates neuronal excitability through STriatal Enriched Protein Tyrosine phosphatase (STEP61) and Calcium Permeable AMPARs in an NMDAR-dependent manner

Alzheimer's disease (AD) is a progressive neurodegenerative disease marked by the accumulation of amyloid-β (Aβ) plaques and neurofibrillary tangles. Aβ oligomers cause synaptic dysfunction early in AD by enhancing long-term depression (LTD; a paradigm for forgetfulness) via metabotropic glutamate receptor (mGluR)-dependent regulation of striatal-enriched tyrosine phosphatase (STEP61). Reelin is a neuromodulator that signals through ApoE (apolipoprotein E) receptors to protect the synapse against Aβ toxicity (Durakoglugil et al., 2009) Reelin signaling is impaired by ApoE4, the most important genetic risk factor for AD, and Aβ-oligomers activate metabotropic glutamate receptors (Renner et al., 2010). We therefore asked whether Reelin might also affect mGluR-LTD. To this end, we induced chemical mGluR-LTD using DHPG (Dihydroxyphenylglycine), a selective mGluR5 agonist. We found that exogenous Reelin reduces the DHPG-induced increase in STEP61, prevents the dephosphorylation of GluA2, and concomitantly blocks mGluR-mediated LTD. By contrast, Reelin deficiency increased expression of Ca2+-permeable GluA2-lacking AMPA receptors along with higher STEP61 levels, resulting in occlusion of DHPG-induced LTD in hippocampal CA1 neurons. We propose a model in which Reelin modulates local protein synthesis as well as AMPA receptor subunit composition through modulation of mGluR-mediated signaling with implications for memory consolidation or neurodegeneration.SIGNIFICANCE STATEMENT Reelin is an important neuromodulator, which in the adult brain controls synaptic plasticity and protects against neurodegeneration. Amyloid-β has been shown to use mGluRs to induce synaptic depression through endocytosis of NMDA and AMPA receptors, a mechanism referred to as LTD, a paradigm of forgetfulness. Our results show that Reelin regulates the phosphatase STEP, which plays an important role in neurodegeneration, as well as the expression of calcium-permeable AMPA receptors, which play a role in memory formation. These data suggest that Reelin uses mGluR LTD pathways to regulate memory formation as well as neurodegeneration

Visualization 6: Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy

Visualization 6 Originally published in Optica on 20 February 2017 (optica-4-2-263

Regulation of Foxo-1 and the angiopoietin-2/Tie2 system by shear stress

Transcription factor Foxo-1 can be inactivated via Akt-mediated phosphorylation. Since shear stress activates Akt, we determined whether Foxo-1 and the Foxo-1-dependent, angiogenesis-related Ang-2/Tie2-system are influenced by shear stress in endothelial cells. Expression of Foxo-1 and its target genes p27Kip1 and Ang-2 was decreased under shear stress (6dyn/cm(2), 24h), nuclear exclusion of Foxo-1 by phosphorylation increased. eNOS and Tie2 were upregulated. No effects on Ang-1 expression were detected. In conclusion, Foxo-1 and Ang-2/Tie2 are part of the molecular response to shear stress, which may regulate angiogenesis

Visualization 5: Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy

Visualization 5 Originally published in Optica on 20 February 2017 (optica-4-2-263

Normal brain development in <i>Lrp4</i>^{<i>ECD/ECD</i>} and <i>Lrp4</i>^<i>-/-</i> mice.

<p><b>A-D</b>: Sagittal slices of the <i>Lrp4</i>^{<i>ECD/ECD</i>} (A,C) and wild type (<i>Lrp4</i>^<i>+/+</i> B,D) mouse cerebellum labeled with NeuN (green), Brn1 (red) and DAPI (blue). Brn1 and NeuN are commonly used markers to label neurons. ML = molecular layer, PL = Purkinje cell layer, and GCL = granule cell layer are clearly distinguishable and not different in the cerebellum of <i>Lrp4</i>^{<i>ECD/ECD</i>} and <i>Lrp4</i>^<i>+/+</i> adult mice (>2 months). <b>E-H</b>: Coronal sections of <i>Lrp4</i>^{<i>ECD/ECD</i>} (E,F) and <i>Lrp4</i>^<i>+/+</i> (G,H) brains showing hippocampus (E,G) and somatosensory cortex (F,H). Slices are labeled for NeuN and DAPI to visualize normal cortical lamination (layers I-VI). I-N: Coronal sections of E18.5 <i>Lrp4</i>^<i>-/-</i> brains compared to their wild type litter mates. Brn1 (I,J) and GFAP (K,L) immunoreactivity in the cortex and hippocampus and Tbr1 plus NeuN double labeling in the cortex are illustrated. Scale bars = 200 μm (A-H), 400 μm (I-L), 100 μm (M,N).</p

Generation and Characterization of an <i>Nse</i>-<i>CreER^T2</i> Transgenic Line Suitable for Inducible Gene Manipulation in Cerebellar Granule Cells

<div><p>We created an <i>Nse</i>-<i>CreER^T2</i> mouse line expressing the tamoxifen-inducible CreER^T2 recombinase under the control of the neuron-specific enolase (<i>Nse</i>) promoter. By using Cre reporter lines we could show that this <i>Nse</i>-<i>CreER^T2</i> line has recombination activity in the granule cells of all cerebellar lobules as well as in postmitotic granule cell precursors in the external granular layer of the developing cerebellum. A few hippocampal dentate gyrus granule cells showed Cre-mediated recombination as well. Cre activity could be induced in both the developing and adult mouse brain. The established mouse line constitutes a valuable tool to study the function of genes expressed by cerebellar granule cells in the developing and adult brain. In combination with reporter lines it is a useful model to analyze the development and maintenance of the cerebellar architecture including granule cell distribution, migration, and the extension of granule cell fibers <i>in vivo</i>.</p></div