Biomolecule surface patterning may enhance membrane association

Under dehydration conditions, amphipathic Late Embryogenesis Abundant (LEA) proteins fold spontaneously from a random conformation into alpha-helical structures and this transition is promoted by the presence of membranes. To gain insight into the thermodynamics of membrane association we model the resulting alpha-helical structures as infinite rigid cylinders patterned with hydrophobic and hydrophilic stripes oriented parallel to their axis. Statistical thermodynamic calculations using Single Chain Mean Field (SCMF) theory show that the relative thickness of the stripes controls the free energy of interaction of the alpha-helices with a phospholipid bilayer, as does the bilayer structure and the depth of the equilibrium penetration of the cylinders into the bilayer. The results may suggest the optimal thickness of the stripes to mimic the association of such protein with membranes.Comment: Published in ACS Nano http://pubs.acs.org/doi/pdf/10.1021/nn204736

General model of phospholipid bilayers in fluid phase within the single chain mean field theory

Coarse-grained model for saturated (DCPC, DLPC, DMPC, DPPC, DSPC) and unsaturated (POPC, DOPC) phospholipids is introduced within the Single Chain Mean Field theory. A single set of parameters adjusted for DMPC bilayers gives an adequate description of equilibrium and mechanical properties of a range of saturated lipid molecules that differ only in length of their hydrophobic tails and unsaturated (POPC, DOPC) phospholipids which have double bonds in the tails. A double bond is modeled with a fixed angle of 120 degrees, while the rest of the parameters are kept the same as saturated lipids. The thickness of the bilayer and its hydrophobic core, the compressibility and the equilibrium area per lipid correspond to experimentally measured values for each lipid, changing linearly with the length of the tail. The model for unsaturated phospholipids also fetches main thermodynamical properties of the bilayers. This model is used for an accurate estimation of the free energies of the compressed or stretched bilayers in stacks or multilayers and gives reasonable estimates for free energies. The proposed model may further be used for studies of mixtures of lipids, small molecule inclusions, interactions of bilayers with embedded proteins

Blood filling and flow in lungs during change in body position in space

In the horizontal position (supine and lateral), in the upright position (head up and head down) and during change of the cat body position in space, quantitative responses of regional blood volume and blood flow in the lungs (ml/100 cu cm) revealed presence of the gradient in the gravitation direction. Blood volume and blood flow of different lung portions changed qualitatively and quantitatively in different ways. These changes occurred only in the direction producing the equality of regional hydrostatical and hemodynamic loads in the lungs at either horizontal level

Can a Carbon Nanotube Pierce through a Phospholipid Bilayer?

Great efficiency to penetrate into living cells is attributed to carbon nanotubes due to a number of direct and indirect observations of carbon nanotubes inside the cells. However, a direct evidence of physical translocation of nanotubes through phospholipid bilayers and the exact microscopic mechanism of their penetration into cells are still lacking. In order to test one of the inferred translocation mechanisms, namely the spontaneous piercing through the membrane induced only by thermal motion, we calculate the energy cost associated with the insertion of a carbon nanotube into a model phospholipid bilayer using the Single Chain Mean Field theory which is particularly suitable for the accurate measurements of equilibrium free energies. We find that the energy cost of the bilayer rupture is quite high compared to the energy of thermal motion. This conclusion may indirectly support other energy dependent translocation mechanisms such as, for example, endocytosis.Comment: Published in ACS Nan

Spectropolarimetric Observations of Herbig Ae/Be Stars. II. Comparison of Spectropolarimetric Surveys: HAeBe, Be and Other Emission-Line Stars

The polarization of light across individual spectral lines contains information about the circumstellar environment on very small spatial scales. We have obtained a large number of high precision, high resolution spectropolarimetric observations of Herbig Ae/Be, Classical Be and other emission-line stars collected on 117 nights of observations with the HiVIS spectropolarimeter at a resolution of R=13000 on the 3.67m AEOS telescope. We also have many observations from the ESPaDOnS spectropolarimeter at a resolution of R=68000 on the 3.6m CFH telescope. In roughly ~2/3 of the so-called "windy" or "disky" Herbig Ae/Be stars, the detected H-alpha linear polarization varies from our typical detection threshold near 0.1% to over 2%. In all but one HAe/Be star the detected polarization effect is not coincident with the H-alpha emission peak but is detected in and around the obvious absorptive part of the line profile. The qu-loops are dominated by the polarization in this absorptive region. In several stars the polarization varies in time mostly in the absorptive component and is not necessarily tied to corresponding variations in intensity. This is a new result not seen at lower resolution. In the Be and emission-line stars, 10 out of a sample of 30 show a typical broad depolarization effect but 4 of these 10 show weaker effects only visible at high resolution. Another 5 of 30 show smaller amplitude, more complex signatures. Six stars of alternate classification showed large amplitude (1-3%) absorptive polarization effects. These detections are largely inconsistent with the traditional disk-scattering and depolarization models.Comment: Published in ApJS 180. 47 pages, 34 figures. Small corrections made to tex

Evidence for weak magnetic fields in early-type emission stars

We report the results of our study of magnetic fields in a sample of 15 Be stars using spectropolarimetric data obtained at the European Southern Observatory with the multi-mode instrument FORS1 installed at the 8m Kueyen telescope. We detect weak photospheric magnetic fields in four stars, HD56014, HD148184, HD155806, and HD181615. We note that for HD181615 the evolutionary status is not obvious due to the fact that it is a binary system currently observed in the initial rapid phase of mass exchange between the two components. Further, we notify the possible presence of distinct circular polarisation features in the circumstellar components of Ca II H&K in three stars, HD58011, HD117357, and HD181615, hinting at a probable presence of magnetic fields in the circumstellar mass loss disks of these stars. We emphasize the need for future spectropolarimetric observations of Be stars with detected magnetic fields to study the temporal evolution of their magnetic fields and the correlation of magnetic field properties with dynamical phenomena taking place in the gaseous circumstellar disks of these stars.Comment: 4 pages, 3 figures, 1 table, accepted for publication in A