Heart Rate Variability based Classification of Normal and Hypertension Cases by Linear-nonlinear Method

The aim of this study is to analyse and compare the heart rate variability (HRV) of normal and hypertension cases using time domain, frequency domain, and nonlinear methods. For short term HRV analysis, a five-minute electrocardiogram (ECG) of 57 normal and 56 hypertension subjects were recorded with prior verification of their clinical status by a cardiologist. Most time domain features of hypertension cases have clearly reduced values over normal subjects, frequency domain features, like power in different spectral bands, also have the distinguishable decreased values, whereas sympathovagal balance has clear edge over hypertension cases than normal cases. Nonlinear parameters of Poincare plot, approximate entropy and sample entropy, have higher values in normal cases when compared with hypertension cases. Support vector machine-based binary system classifies these two classes with 100 per cent accuracy and 100 per cent sensitivity when all time domain, frequency domain, and nonlinear features were used. It may work as a better predictor for in patients with hypertension.Science Journal, Vol. 64, No. 6, November 2014, pp.542-548, DOI:http://dx.doi.org/10.14429/dsj.64.786

Combined Toxicity and Bioconcentration of Fluoride and Arsenic in African Catfish Clarias gariepinus (Burchell, 1822)

Laboratory experiments were performed to examine the combined toxic effects of two important aquatic contaminants viz., arsenic and fluoride on African catfish, Clarias gariepinus. Additionally, the bio concentration factors (BCFs) of the two contaminants in tissues and blood of catfish were also determined. The LC50 for sodium fluoride and arsenic trioxide were determined to be 619.3 mg L-1, 30.3 mg L-1, respectively. Erratic swimming movements with hyperactivity, loss of equilibrium, augmented air gulping and decreased food consumption were observed in the experimental groups. In co-exposure groups of arsenic and fluoride, the concentration of fluoride in fish tissues increased with increasing water fluoride concentration in the test aquaria with significant differences (

A Low-spin Mn(III) Complex

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Gold Market Analyzer using Selection based Algorithm

Gold is the most important and valuable element right from its discovery. It is the most significant element at present and also the most valuable asset to. In the present market scenario the investors use gold as the security for their shares investment. During International trade all the countries use gold as their main mode of transaction. It is seen that all the currencies accepted by the International market are having the gold as the backup for their economy. The prices of gold are rising day by day continuously. As we see in the history of gold market the present prices of gold are much high as compare to the past values and that's why the gold market has attracted the most attention. The paper focus on the continuous changing in the gold rates, investment policies depend on the forecasting of trends in gold which will help the data mining companies to minimize the risk The description of the future situation on the basis of present trends is just not limited to the forecasting the prices. The knowledge discovers by the data mining techniques is gathered from the different gold related websites and also from the jewellers database. It is much more important for the ornaments making companies to know the demand and the requirements for the ornaments during the unstable (uncertain) market conditions. For the classification purpose the maid and sale database was gathered from the nearest jewellery shops of the past 5 years. The prediction is done after complete analysis of the gathered data set. With this the paper concentrate on making the information available about the government and private schemes related to gold market on one place. The paper proposes the system that gives the total access to the registered user and limited access to the unregistered user to get the required information of gold. The latest updates are provided to the registered user by sending mail or text message when the user was offline

Isolation of Myogenic Stem Cells from Cultures of Cryopreserved Human Skeletal Muscle

We demonstrate that subpopulations of adult human skeletal muscle-derived stem cells, myogenic endothelial cells (MECs), and perivascular stem cells (PSCs) can be simultaneously purified by fluorescence-activated cell sorting (FACS) from cryopreserved human primary skeletal muscle cell cultures (cryo-hPSMCs). For FACS isolation, we utilized a combination of cell lineage markers: the myogenic cell marker CD56, the endothelial cell marker UEA-1 receptor (UEA-1R), and the perivascular cell marker CD146. MECs expressing all three cell lineage markers (CD56+UEA-1R+CD146+/CD45+) and PSCs expressing only CD146 (CD146+/CD45+CD56+UEA-1R+) were isolated by FACS. To evaluate their myogenic capacities, the sorted cells, with and without expansion in culture, were transplanted into the cardiotoxin-injured skeletal muscles of immunodeficient mice. The purified MECs exhibited the highest regenerative capacity in the injured mouse muscles among all cell fractions tested, while PSCs remained superior to myoblasts and the unpurified primary skeletal muscle cells. Our findings show that both MECs and PSCs retain their high myogenic potentials after in vitro expansion, cryopreservation, and FACS sorting. The current study demonstrates that myogenic stem cells are prospectively isolatable from long-term cryopreserved primary skeletal muscle cell cultures. We emphasize the potential application of this new approach to extract therapeutic stem cells from human muscle cells cryogenically banked for clinical purposes. © 2012 Cognizant Comm. Corp

Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq

Genome editing has shown great promise for clinical translation but also revealed the risk of genotoxicity caused by off-target effects of programmable nucleases. Here we describe chromosomal aberrations analysis by single targeted linker-mediated PCR sequencing (CAST-Seq), a preclinical assay to identify and quantify chromosomal aberrations derived from on-target and off-target activities of CRISPR-Cas nucleases or transcriptional activator-like effector nucleases (TALENs), respectively, in human hematopoietic stem cells (HSCs). Depending on the employed designer nuclease, CAST-Seq detected translocations in 0%–0.5% of gene-edited human CD34+ HSCs, and up to 20% of on-target loci harbored gross rearrangements. Moreover, CAST-Seq detected distinct types of chromosomal aberrations, such as homology-mediated translocations, that are mediated by homologous recombination and not off-target activity. CAST-Seq is a sensitive assay able to identify and quantify unintended chromosomal rearrangements in addition to the more typical mutations at off-target sites. CAST-Seq analyses may be particularly relevant for therapeutic genome editing to enable thorough risk assessment before clinical application of gene-edited products

ATR inhibition facilitates targeting of leukemia dependence on convergent nucleotide biosynthetic pathways.

Leukemia cells rely on two nucleotide biosynthetic pathways, de novo and salvage, to produce dNTPs for DNA replication. Here, using metabolomic, proteomic, and phosphoproteomic approaches, we show that inhibition of the replication stress sensing kinase ataxia telangiectasia and Rad3-related protein (ATR) reduces the output of both de novo and salvage pathways by regulating the activity of their respective rate-limiting enzymes, ribonucleotide reductase (RNR) and deoxycytidine kinase (dCK), via distinct molecular mechanisms. Quantification of nucleotide biosynthesis in ATR-inhibited acute lymphoblastic leukemia (ALL) cells reveals substantial remaining de novo and salvage activities, and could not eliminate the disease in vivo. However, targeting these remaining activities with RNR and dCK inhibitors triggers lethal replication stress in vitro and long-term disease-free survival in mice with B-ALL, without detectable toxicity. Thus the functional interplay between alternative nucleotide biosynthetic routes and ATR provides therapeutic opportunities in leukemia and potentially other cancers.Leukemic cells depend on the nucleotide synthesis pathway to proliferate. Here the authors use metabolomics and proteomics to show that inhibition of ATR reduced the activity of these pathways thus providing a valuable therapeutic target in leukemia

Synthesis and characterization of core-shell structure silica-coated Fe29.5Ni70.5 nanoparticles

In view of potential applications of magnetic particles in biomedicine and electromagnetic devices, we made use of the classical Stober method base-catalysed hydrolysis and condensation of tetraethoxysilane (TEOS) to encapsulate FeNi nanoparticles within a silica shell. An original stirring system under high power ultrasounds made possible to disperse the otherwise agglomerated particles. Sonication guaranteed particles to remain dispersed during the Stober synthesis and also improved the efficiency of the method. The coated particles are characterized by electron microscopy (TEM) and spectroscopy (EDX) showing a core-shell structure with a uniform layer of silica. Silica-coating does not affect the core magnetic properties. Indeed, all samples are ferromagnetic at 77 K and room temperature and the Curie point remains unchanged. Only the coercive force shows an unexpected non-monotonous dependence on silica layer thickness.Comment: Regular paper submited to international peer-reveiwed journa

Cascaded complementary filter architecture for sensor fusion in attitude estimation

Copyright: © 2021 by the authors. Attitude estimation is the process of computing the orientation angles of an object with respect to a fixed frame of reference. Gyroscope, accelerometer, and magnetometer are some of the fundamental sensors used in attitude estimation. The orientation angles computed from these sensors are combined using the sensor fusion methodologies to obtain accurate estimates. The complementary filter is one of the widely adopted techniques whose performance is highly dependent on the appropriate selection of its gain parameters. This paper presents a novel cascaded architecture of the complementary filter that employs a nonlinear and linear version of the complementary filter within one framework. The nonlinear version is used to correct the gyroscope bias, while the linear version estimates the attitude angle. The significant advantage of the proposed architecture is its independence of the filter parameters, thereby avoiding tuning the filter’s gain parameters. The proposed architecture does not require any mathematical modeling of the system and is computationally inexpensive. The proposed methodology is applied to the real-world datasets, and the estimation results were found to be promising compared to the other state-of-the-art algorithms

Scaling of the Equilibrium Magnetization in the Mixed State of Type-II Superconductors

We discuss the analysis of mixed-state magnetization data of type-II superconductors using a recently developed scaling procedure. It is based on the fact that, if the Ginzburg-Landau parameter kappa does not depend on temperature, the magnetic susceptibility is a universal function of H/H_c2(T), leading to a simple relation between magnetizations at different temperatures. Although this scaling procedure does not provide absolute values of the upper critical fieldH_c2(T), its temperature variation can be established rather accurately. This provides an opportunity to validate theoretical models that are usually employed for the evaluation of H_c2(T) from equilibrium magnetization data. In the second part of the paper we apply this scaling procedure for a discussion of the notorious first order phase transition in the mixed state of high temperature superconductors. Our analysis, based on experimental magnetization data available in the literature, shows that the shift of the magnetization accross the transition may adopt either sign, depending on the particular chosen sample. We argue that this observation is inconsistent with the interpretation that this transition always represents the melting transition of the vortex lattice.Comment: 18 pages, 12 figure