Nonrelativistic fermions with holographic interactions and the unitary Fermi gas

We present an alternative way of computing nonrelativistic single-particle spectra from holography. To this end, we introduce a mass gap in a holographic Dirac semimetal and subsequently study the nonrelativistic limit of the resulting spectral functions. We use this method to compute the momentum distributions and the equation of state of our nonrelativistic fermions, of which the latter can be used to extract all thermodynamic properties of the system. We find that our results are universal and reproduce many experimentally and theoretically known features of an ultracold Fermi gas at unitarity.Comment: 5+6 pages, 4+2 figure

Order parameter fluctuations in the holographic superconductor

We investigate the effect of order parameter fluctuations in the holographic superconductor. In particular, using a fully backreacted bulk geometry, the intrinsic spectral functions of the order parameter in both the normal and the superconducting phase are computed. We also present a vector-like large-$N$ version of the Ginzburg-Landau model that accurately describes our long-wavelength results in both phases. The large-$N$ limit of the latter model explains why the Higgs mode and the second-sound mode are not present in the spectral functions. Our results indicate that the holographic superconductor describes a relativistic multi-component superfluid in the universal regime of the BEC-BCS crossover.Comment: 21 pages, 15 figures; version accepted for publicatio

Precise Control of Molecular Self-Diffusion in Isoreticular and Multivariate Metal-Organic Frameworks.

Understanding the factors that affect self-diffusion in isoreticular and multivariate (MTV) MOFs is key to their application in drug delivery, separations, and heterogeneous catalysis. Here, we measure the apparent self-diffusion of solvents saturated within the pores of large single crystals of MOF-5, IRMOF-3 (amino-functionalized MOF-5), and 17 MTV-MOF-5/IRMOF-3 materials at various mole fractions. We find that the apparent self-diffusion coefficient of N,N-dimethylformamide (DMF) may be tuned linearly between the diffusion coefficients of MOF-5 and IRMOF-3 as a function of the linker mole fraction. We compare a series of solvents at saturation in MOF-5 and IRMOF-3 to elucidate the mechanism by which the linker amino groups tune molecular diffusion. The ratio of the self-diffusion coefficients for solvents in MOF-5 to those in IRMOF-3 is similar across all solvents tested, regardless of solvent polarity. We conclude that average pore aperture, not solvent-linker chemical interactions, is the primary factor responsible for the different diffusion dynamics upon introduction of an amino group to the linker

Modeling Pichia pastoris Growth on Methanol and Optimizing the Production of a Recombinant Protein, the Heavy-Chain Fragment C of Botulinum Neurotoxin, Serotype A

An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut+ expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(Hc)], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities. The model describes the relationships between specific growth rate and methanol concentration, and the relationships between specific methanol and ammonium consump-tion rates and specific growth rate under methanol-limited growth conditions. The maximum specific growth rate (μ) determined from the model was 0.08 h−1 at a methanol concentration of 3.65 g/L, while the actual maximum μ was 0.0709 h−1. The maximum specific methanol consumption rate was 0.0682 g/g WCW/h. From the model, growth can be defined as either methanol-limited or metha-nol-inhibited and is delineated at a methanol concentration of 3.65 g/L. Under inhibited conditions, the observed biomass yield (YX/MeOH) was lower and the maintenance coefficient (mMeOH) was higher than compared to limited methanol conditions. The YX/MeOH decreased and mMeOH increased with increasing methanol concen-tration under methanolinhibited conditions. BoNT/A(Hc) content in cells (a) under inhibited growth was lower than that under limited growth, and decreased with increasing methanol concentration. A maximum a of 1.72 mg/g WCW was achieved at a μ of 0.0267 h−1 and induction time of 12 h

Crystallization and preliminary X-ray analysis of \u3ci\u3eNa\u3c/i\u3e-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite \u3ci\u3eNecator americanus\u3c/i\u3e

Human hookworm infection is a major cause of anemia and malnutrition in the developing world. In an effort to control hookworm infection, the Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective larval stage (L3) of the parasite, including a family of pathogenesis-related-1 (PR-1) proteins known as the ancylostoma-secreted proteins (ASPs). The functions of the ASPs are unknown. In addition, it is unclear why some ASPs have one while others have multiple PR-1 domains. There are no known structures of a multi-domain ASP and in an effort to remedy this situation, recombinant Na-ASP-1 has been expressed, purified and crystallized. Na-ASP-1 is a 406-amino-acid multi-domain ASP from the prevalent human hookworm parasite Necator americanus. Useful X-ray data to 2.2 A ° have been collected from a crystal that belongs to the monoclinic space group P21 with unit-cell parameters a = 67.7, b = 74.27, c = 84.60 Å, β = 112.12°. An initial molecular-replacement solution has been obtained with one monomer in the asymmetric unit

Crystallization and preliminary X-ray analysis of \u3ci\u3eNa\u3c/i\u3e-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite \u3ci\u3eNecator americanus\u3c/i\u3e

Human hookworm infection is a major cause of anemia and malnutrition in the developing world. In an effort to control hookworm infection, the Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective larval stage (L3) of the parasite, including a family of pathogenesis-related-1 (PR-1) proteins known as the ancylostoma-secreted proteins (ASPs). The functions of the ASPs are unknown. In addition, it is unclear why some ASPs have one while others have multiple PR-1 domains. There are no known structures of a multi-domain ASP and in an effort to remedy this situation, recombinant Na-ASP-1 has been expressed, purified and crystallized. Na-ASP-1 is a 406-amino-acid multi-domain ASP from the prevalent human hookworm parasite Necator americanus. Useful X-ray data to 2.2 A ° have been collected from a crystal that belongs to the monoclinic space group P21 with unit-cell parameters a = 67.7, b = 74.27, c = 84.60 Å, β = 112.12°. An initial molecular-replacement solution has been obtained with one monomer in the asymmetric unit

Crystallization and preliminary X-ray analysis of Na-ASP-1, a multi-domain pathogenesis-related-1 protein from the human hookworm parasite Necator americanus. Corrigendum

A correction to the paper by Asojo et al. [(2005), Acta Cryst. F61, 391–394]

Pichia pastoris fermentation with mixed-feeds of glycerol and methanol: growth kinetics and production improvement

Fed-batch fermentation of a methanol utilization plus (Mut+) Pichia pastoris strain typically has a growth phase followed by a production phase (induction phase). In the growth phase glycerol is usually used as carbon for cell growth while in the production phase methanol serves as both inducer and carbon source for recombinant protein expression. Some researchers employed a mixed glycerol-methanol feeding strategy during the induction phase to improve production, but growth kinetics on glycerol and methanol and the interaction between them were not reported. The objective of this paper is to optimize the mixed feeding strategy based on growth kinetic studies using a Mut+ Pichia strain, which expresses the heavy-chain fragment C of botulinum neurotoxin serotype C [BoNT/C(Hc)] intracellularly, as a model system. Growth models on glycerol and methanol that describe the relationship between specific growth rate (μ) and specific glycerol/ methanol consumption rate (vgly,vMeOH) were established. A mixed feeding strategy with desired μgly/ μMeOH =1, 2, 3, 4 (desired μMeOH set at 0.015 hm-1) was employed to study growth interactions and their effect on production. The results show that the optimal desired μgly/μMeOH is around 2 for obtaining the highest BoNT/C(Hc) protein content in cells: about 3 mg/g wet cells

Massive Dirac fermions from holography

We provide a framework to compute the dynamics of massive Dirac fermions using holography. To this end we consider two bulk Dirac fermions that are coupled via a Yukawa interaction and propagate on a gravitational background in which a mass deformation is introduced. Moreover, we discuss the incorporation of this approach in semiholography. The resulting undoped fermionic spectral functions indeed show that the Yukawa coupling induces a gap in the holographic spectrum, whereas the semiholographic extension is in general gapped but additionally contains a quantum critical point at which the effective fermion mass vanishes and a topological phase transition occurs. Furthermore, when introducing doping, the fermionic spectral functions show a quantum phase transition between a gapped material and a Fermi liquid

Massive Dirac fermions from holography

We provide a framework to compute the dynamics of massive Dirac fermions using holography. To this end we consider two bulk Dirac fermions that are coupled via a Yukawa interaction and propagate on a gravitational background in which a mass deformation is introduced. Moreover, we discuss the incorporation of this approach in semiholography. The resulting undoped fermionic spectral functions indeed show that the Yukawa coupling induces a gap in the holographic spectrum, whereas the semiholographic extension is in general gapped but additionally contains a quantum critical point at which the effective fermion mass vanishes and a topological phase transition occurs. Furthermore, when introducing doping, the fermionic spectral functions show a quantum phase transition between a gapped material and a Fermi liquid