Working precarious careers trajectories: tracing neoliberal discourses in younger workers’ narratives

© 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group. The aim of this article is to explore how, in the context of the post Global Financial Crisis (GFC), some Italian younger workers in a specific geographical region in Italy recount their work trajectories. Drawing on narrative interviews with ten participants (aged between 24 and 30) as part of a research project carried out in the Autonomous region of Aosta Valley in Italy, the article traces discourses closely associated with neoliberalism–the discourses of the entrepreneurial self, employability and self-responsibilisation–through which subjects’ work experiences take shape. Moreover, the analysis highlights how locality, one’s personal relation with the geographical territory, makes more complex the younger people’s negotiations in crafting themselves vis-à-vis precarious employment opportunities and wider socio-economic dynamics in respect of precarious employment opportunities

Coming Out, But Into What? Problematizing Discursive Variations of Revealing the Gay Self in the Workplace

There is a substantial mainstream literature on coming out in organizations, which investigates the positive effects for gay people of being out at work, but very few contributions that challenge the discourse of coming out. Taking as its starting point Butler's famous question ‘So we are out of the closet but into what?’, this paper problematizes coming out discourses in the workplace. We report on a study in which ten men were invited to talk about their coming out in the workplace. There were three main ways through which our participants constituted themselves as gay men when they talked about coming out: by defining themselves as, and admitting to, being gay; by introducing themselves as being in a gay relationship; and by adopting legitimate subject positions such as the Other, the different one, or the normal gay. Through our analysis, discussions and conclusions, we show how participants position themselves within different discursive variations, thus revealing the multiplicity of ‘the gay self’ and highlighting how coming out repeats and supports normative systems

Cluster J Mycobacteriophages: Intron Splicing in Capsid and Tail Genes

Bacteriophages isolated on Mycobacterium smegmatis mc2155 represent many distinct genomes sharing little or no DNA sequence similarity. The genomes are architecturally mosaic and are replete with genes of unknown function. A new group of genomes sharing substantial nucleotide sequences constitute Cluster J. The six mycobacteriophages forming Cluster J are morphologically members of the Siphoviridae, but have unusually long genomes ranging from 106.3 to 117 kbp. Reconstruction of the capsid by cryo-electron microscopy of mycobacteriophage BAKA reveals an icosahedral structure with a triangulation number of 13. All six phages are temperate and homoimmune, and prophage establishment involves integration into a tRNA-Leu gene not previously identified as a mycobacterial attB site for phage integration. The Cluster J genomes provide two examples of intron splicing within the virion structural genes, one in a major capsid subunit gene, and one in a tail gene. These genomes also contain numerous free-standing HNH homing endonuclease, and comparative analysis reveals how these could contribute to genome mosaicism. The unusual Cluster J genomes provide new insights into phage genome architecture, gene function, capsid structure, gene mobility, intron splicing, and evolution. © 2013 Pope et al

Histone deacetylases in viral infections

Chromatin remodeling and gene expression are regulated by histone deacetylases (HDACs) that condense the chromatin structure by deacetylating histones. HDACs comprise a group of enzymes that are responsible for the regulation of both cellular and viral genes at the transcriptional level. In mammals, a total of 18 HDACs have been identified and grouped into four classes, i.e., class I (HDACs 1, 2, 3, 8), class II (HDACs 4, 5, 6, 7, 9, 10), class III (Sirt1–Sirt7), and class IV (HDAC11). We review here the role of HDACs on viral replication and how HDAC inhibitors could potentially be used as new therapeutic tools in several viral infections