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Primary brain CD30+ ALK1+ anaplastic large cell lymphoma ('ALKoma'): the first case with a combination of 'not common' variants

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Hystological and ultrastructural study of opercular anomalies in gilthead sea bream larvae (Sparus aurata)

The high incidence of opercular deformities appearing in Mediterranean marine hatcheries is an important problem which is causing considerable economic losses. The aetiology of this syndrome is not yet well understood. In this study a histological and ultrastructural description of gilthead sea bream larvae (Sparus aurata) affected with anomalies of the opercular complex was carried out. The first opercular alterations were detected 17 days after hatching, when the opercular support in the branchio-cranial area was still cartilaginous and the only structure of the opercle is a connective laminae composed of a dense connective tissue. No degenerative alterations or inflammatory processes were found in the opercular tissues. The electron microscopy observations on 30 days-old larvae allowed the identification of abnormality in the mineral deposition within the dermal ossification in curled opercles. These preliminary observations suggest that an early alteration in the connective tissue formation and an abnormal mineralisation of the fibrous bone can predispose opercular folding

A preliminary histological and ultrastructural study of opercular anomalies in gilthead sea bream larvae (Sparus aurata)

The high prevalence of opercular deformities present in fish from Mediterranean marine hatcheries is an important problem, which is causing considerable economic losses. The aetiology of this syndrome is not yet well understood. In this study a histological and ultrastructural description of gilthead sea bream (Sparus aurata) larvae affected with anomalies of the opercular complex was carried out. Samples of larvae were obtained from a marine hatchery located in north-eastern Italy. For each sample, 100 larvae (age range day 0 to 90) were fixed for histological and ultrastructural observations. The first opercular alterations were detected 17 days after hatching, when the opercular support in the branchio-cranial area is still cartilaginous and the only structure of the opercle is a connective lamina composed of a dense connective tissue. Deformation could affect one or both opercles. No degenerative alterations or inflammatory processes were found in the opercular tissues. The electron microscopy (TEM) observations on 30 day old larvae allowed the identification of mineral deposition abnormalities within the dermal ossification in curled opercles. The mineralization process appears to be very heterogeneous and irregular, in some cases being less abundant than normally detected. These preliminary observations suggest that an early alteration in the connective tissue formation and an abnormal mineralization of the fibrous bone can predispose opercular folding

Hystological and ultrastructural study of opercular anomalies in gilthead sea bream larvae (Sparus aurata).

The high incidence of opercular deformities appearing in Mediterranean marine hatcheries is an important problem which is causing considerable economic losses. The aetiology of this syndrome is not yet well understood. In this study a histological and ultrastructural description of gilthead sea bream larvae (Sparus aurata) affected with anomalies of the opercular complex was carried out. The first opercular alterations were detected 17 days after hatching, when the opercular support in the branchio-cranial area was still cartilaginous and the only structure of the opercle is a connective laminae composed of a dense connective tissue. No degenerative alterations or inflammatory processes were found in the opercular tissues. The electron microscopy observations on 30 days-old larvae allowed the identification of abnormality in the mineral deposition within the dermal ossification in curled opercles. These preliminary observations suggest that an early alteration in the connective tissue formation and an abnormal mineralisation of the fibrous bone can predispose opercular folding

Antimicrobial photodynamic action on dentin using a light-emitting diode light source

Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin. Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2). Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem. Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)CePOFPIBIC-CNP

PROTEOMIC STUDIES ON LOW- AND HIGH-GRADE HUMAN BRAIN ASTROCYTOMAS

Human brain astrocytomas range from the indolent low-grade to the highly infiltrating and aggressive high-grade form, also known as glioblastoma multiforme. The extensive heterogeneity of astrocytic tumors complicates their pathological classification. In this study, we compared the protein pattern of low-grade fibrillary astrocytomas to that of glioblastoma multiforme by 2D electrophoresis. The level of most proteins remains unchanged between the different grade tumors and only few differences are reproducibly observable. Fifteen differentially expressed proteins, as well as seventy conserved spots, were identified by mass spectrometry. Western and immnunohistochemical analysis confirmed the differential expression of the identified proteins. These data provide an initial reference map for brain gliomas. Among the proteins more highly expressed in glioblastoma multiforme, we found peroxire- doxin 1 and 6, the transcription factor BTF3, and R-B-crystallin, whereas protein disulfide is

Effects of Delayed Hypothermic Machine Perfusion on Kidney Grafts with a Preliminary Period of Static Cold Storage and a Total Cold Ischemia Time of Over 24 Hours

BACKGROUND Hypothermic machine perfusion (HMP) appears to exert a reconditioning effect on the ischemic damage of kidney grafts. However, some concerns still remain about its real effectiveness when it is delayed after a preliminary period of static cold storage (SCS) or with prolonged overall cold ischemia time (CIT). MATERIAL AND METHODS The effect of HMP on hemodynamic, metabolic, histological and ultrastructural features of grafts was investigated in 21 single-kidney grafts treated with a delayed HMP after SCS and with a total CIT of over 24 h. RESULTS The mean CIT, SCS, and HMP times were 29 h, 12 h, and 18 h, respectively. Longer SCS was associated with higher vascular resistance and lower arterial flow. In the pre- vs. post-HMP comparison, a significant decrease in arterial resistances and increase of flow were recorded. The hemodynamic improvement was independent of HMP duration. The perfused grafts retained some metabolic activity, with a statistically significant decrease of pH, pO2, and glucose levels, and increase of lactates in the perfusion liquid, by the end of HMP. Longer SCS was associated with higher pH and greater pO2 decrease during HMP. Light microscopy and transmission electronic microscopy revealed no significant variations in nuclear, cytoplasmic, or ultrastructural damage. SCS, HMP, and CIT were not identified as risk factor for delayed graft function or rejection. CONCLUSIONS A delayed and extended HMP can recover the graft hemodynamic function, maintain some metabolic activity, and stabilize the accumulated ischemic damage due to a preliminary SCS