25 research outputs found
Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications
In this research, a comparative analysis of results of investigations involving different human
populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is
reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications
in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer
surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium,
and occurrence of inflammatory processes. From each patient, microorganisms isolated of periodontium, dental plaque,
and dental pocket swabs were identified in wet and stained microscopic preparations; standard microbiology in vitro
techniques were also applied to determine the fungal and bacterial strains. The comparative analysis of results of direct
microscopic examinations and in vitro cultures assessment indicated significant differences in prevalences of fungi,
parasitic oral protozoans and bacteriae in particular patient’s groups. Yeast-like fungi belonging to Candida genus,
mostly of C. albicans group, were identified in 93.75% patients with the oral cancer, while in 25% of individuals
assessed without neoplasm. E. gingivalis amoebae were only found in 12.5% patients with the serious disease; no
trichomonads were detected in all patients analyzed. Among bacteria species, potentially pathogenic Enterobacteriaceae
were found in the patients with oral cancer. The pronounced shift in the microbiota species composition in the patients
who needed prolonged treatment due to oral cavity cancer, compared to other generally healthy persons has been
showed in this analysis
Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders
The retrospective analysis of data on oral cavity clinical status in relation to microbiota species
composition is presented. The research regards patients of different age, with and without congenital malformation,
pretreatment assessed for occurrence of pathological changes in the masticatory system. Samples of the swabs collected
from each patient (from dental plaque, periodontium and dental pockets) were used for identification of oral protozoans
in wet slides and stained preparations; additionally, transmission electron microscope examination was performed. The
material was used for in vitro cultures to identify bacteria strains. Clinically, intensity of tissue deteriorations was higher
in patients with a congenital disease. Alive Trichomonas tenax and Entamoeba gingivalis, species with confirmed
pathogenic impact on oral cavity and neighboring structures, were detected with higher prevalence in older patients.
Enterococci, Staphylococcus aureus, various Enterobacteriaceae were more frequently detected in patients with somatic
and mental retardations; in mouths of those patients, Klebsiella pneumonia and Pseudomonas aeruginosa opportunistic
strains occurred. Masticatory system abnormalities favor colonization of oral cavity by exogenous species and
dissemination of infections, especially dangerous for patients with congenital diseases. Oral microbiota assessment and
preventive measures may be helpful to avoid subsequent peri-surgery complications
Identification of infectious microbiota from oral cavity environment of various population group patients as a preventive approach to human health risk factors
Introduction and objective. This study presents the results of comparative investigations aimed to determine microbiota that can occur in the oral environment in different human populations. The objective of the research was to identify pathogenic oral microbiota, the potential cause of health complications in patients of different population groups.
Materials and method. The study included 95 patients requiring dental or surgical treatment; their oral cavity environment microbiota as risk factors of local and general infections were assessed.
Results. In clinical assessment, differences occurred in oral cavity conditions between patients with malformations of the masticatory system, kidney allograft recipients and individuals without indications for surgical procedures. The presence of various pathogenic and opportunistic bacterial strains in oral cavities were revealed by direct microscopic and in vitro culture techniques.
Conclusions. Colonization of oral cavities of patients requiring surgical treatment by the potentially pathogenic bacteria constitutes the threat of their spread, and development of general infections. Assessment of oral cavity infectious microbiota should be performed as a preventive measure against peri-surgical complications