Antifungal Activity and DNA Topoisomerase Inhibition of Hydrolysable Tannins from Punica granatum L

Punica granatum L. (pomegranate) fruit is known to be an important source of bioactive phenolic compounds belonging to hydrolysable tannins. Pomegranate extracts have shown antifungal activity, but the compounds responsible for this activity and their mechanism/s of action have not been completely elucidated up to now. The aim of the present study was the investigation of the inhibition ability of a selection of pomegranate phenolic compounds (i.e., punicalagin, punicalin, ellagic acid, gallic acid) on both plant and human fungal pathogens. In addition, the biological target of punicalagin was identified here for the first time. The antifungal activity of pomegranate phenolics was evaluated by means of Agar Disk Diffusion Assay and minimum inhibitory concentration (MIC) evaluation. A chemoinformatic analysis predicted for the first time topoisomerases I and II as potential biological targets of punicalagin, and this prediction was confirmed by in vitro inhibition assays. Concerning phytopathogens, all the tested compounds were effective, often similarly to the fungicide imazalil at the label dose. Particularly, punicalagin showed the lowest MIC for Alternaria alternata and Botrytis cinerea, whereas punicalin was the most active compound in terms of growth control extent. As for human pathogens, punicalagin was the most active compound among the tested ones against Candida albicans reference strains, as well as against the clinically isolates. UHPLC coupled with HRMS indicated that C. albicans, similarly to the phytopathogen Coniella granati, is able to hydrolyze both punicalagin and punicalin as a response to the fungal attack. Punicalagin showed a strong inhibitory activity, with IC50 values of 9.0 and 4.6 µM against C. albicans topoisomerases I and II, respectively. Altogether, the results provide evidence that punicalagin is a valuable candidate to be further exploited as an antifungal agent in particular against human fungal infections

Antifungal Activity and DNA Topoisomerase Inhibition of Hydrolysable Tannins from Punica granatum L

Punica granatum L. (pomegranate) fruit is known to be an important source of bioactive phenolic compounds belonging to hydrolysable tannins. Pomegranate extracts have shown antifungal activity, but the compounds responsible for this activity and their mechanism/s of action have not been completely elucidated up to now. The aim of the present study was the investigation of the inhibition ability of a selection of pomegranate phenolic compounds (i.e., punicalagin, punicalin, ellagic acid, gallic acid) on both plant and human fungal pathogens. In addition, the biological target of punicalagin was identified here for the first time. The antifungal activity of pomegranate phenolics was evaluated by means of Agar Disk Diffusion Assay and minimum inhibitory concentration (MIC) evaluation. A chemoinformatic analysis predicted for the first time topoisomerases I and II as potential biological targets of punicalagin, and this prediction was confirmed by in vitro inhibition assays. Concerning phytopathogens, all the tested compounds were effective, often similarly to the fungicide imazalil at the label dose. Particularly, punicalagin showed the lowest MIC for Alternaria alternata and Botrytis cinerea, whereas punicalin was the most active compound in terms of growth control extent. As for human pathogens, punicalagin was the most active compound among the tested ones against Candida albicans reference strains, as well as against the clinically isolates. UHPLC coupled with HRMS indicated that C. albicans, similarly to the phytopathogen Coniella granati, is able to hydrolyze both punicalagin and punicalin as a response to the fungal attack. Punicalagin showed a strong inhibitory activity, with IC50 values of 9.0 and 4.6 µM against C. albicans topoisomerases I and II, respectively. Altogether, the results provide evidence that punicalagin is a valuable candidate to be further exploited as an antifungal agent in particular against human fungal infections

Looking for seismites in the Fucino basin: preliminary results from a combined geological geophysical approach.

We present a combined geological-geophysical study on the lacustrine sequence of the Fucino Plain (central Italy). New acquired data on liquefaction features and the recovery of a seismite in the lacustrine sequence are shown. Our preliminary results suggest the occurrence of three seismic events in the last ca. 45 kyr. Moreover, a first attempt to find out the source deposit responsible of the widespread liquefaction phenomena has been performed by means of shallow engine boreholes and ERT profiles

Imaging the three-dimensional architecture of the Middle Aterno basin (2009 L’ Aquila earthquake, Central Italy) using ground TDEM and seismic noise surveys: preliminary results

We present preliminary results from a multidisciplinary geophysical approach ap- plied to the imaging of the three-dimensional architecture of the Middle Aterno basin, close to the epicentral area of the 2009 L’Aquila earthquake (central Italy). We collected several time domain electromagnetic soundings (TDEM) coupled with seismic noise measurements focu- sing on the characterization of the bedrock/in ll interface. Our preliminary results agree with existing geophysical data collected in the area, and show that the southeastern portion of the basin is characterized by a deepening of the Mesozoic-Tertiary bedrock down to a depth of more than 450 m. We found that a joint use of electromagnetic and seismic methods signi - cantly contributes in obtaining new insights on the 3D geometry of the Middle Aterno basin. Moreover, we believe that our combined approach based on TDEM and noise measurements can be adopted to investigate similar geological settings elsewhere.PublishedPescina (AQ), Italy2T. Tettonica attiva3T. Pericolosità sismica e contributo alla definizione del rischio7A. Geofisica di esplorazioneope

Imaging the three-dimensional architecture of the Middle Aterno basin (2009 L’Aquila earthquake, Central Italy) using ground TDEM and seismic noise surveys: preliminary results

We present preliminary results from a multidisciplinary geophysical approach applied to the imaging of the threedimensional architecture of the Middle Aterno basin, close to the epicentral area of the 2009 L’Aquila earthquake (central Italy). We collected several time domain electromagnetic soundings (TDEM) coupled with seismic noise measurements focusing on the characterization of the bedrock/infill interface. Our preliminary results agree with existing geophysical data collected in the area, and show that the southeastern portion of the basin is characterized by a deepening of the Mesozoic-Tertiary bedrock down to a depth of more than 450 m. We found that a joint use of electromagnetic and seismic methods significantly contributes in obtaining new insights on the 3D geometry of the Middle Aterno basin. Moreover, we believe that our combined approach based on TDEM and noise measurements can be adopted to investigate similar geological settings elsewhere.PublishedPescina (AQ)2T. Tettonica attivaope

Contrast-enhanced MR Imaging versus Contrast-enhanced US: A Comparison in Glioblastoma Surgery by Using Intraoperative Fusion Imaging

Purpose To compare contrast material enhancement of glioblastoma multiforme (GBM) with intraoperative contrast-enhanced ultrasonography (US) versus that with preoperative gadolinium-enhanced T1-weighted magnetic resonance (MR) imaging by using real-time fusion imaging. Materials and Methods Ten patients with GBM were retrospectively identified by using routinely collected, anonymized data. Navigated contrast-enhanced US was performed after intravenous administration of contrast material before tumor resection. All patients underwent tumor excision with navigated intraoperative US guidance with use of fusion imaging between real-time intraoperative US and preoperative MR imaging. With use of fusion imaging, glioblastoma contrast enhancement at contrast-enhanced US (regarding location, morphologic features, margins, dimensions, and pattern) was compared with that at gadolinium-enhanced T1-weighted MR imaging. Results Fusion imaging for virtual navigation enabled matching of real-time contrast-enhanced US scans to corresponding coplanar preoperative gadolinium-enhanced T1-weighted MR images in all cases, with a positional discrepancy of less than 2 mm. Contrast enhancement of gadolinium-enhanced T1-weighted MR imaging and contrast-enhanced US was superimposable in all cases with regard to location, margins, dimensions, and morphologic features. The qualitative analysis of contrast enhancement pattern demonstrated a similar distribution in contrast-enhanced US and gadolinium-enhanced T1-weighted MR imaging in nine patients: Seven lesions showed peripheral inhomogeneous ring enhancement, and two lesions showed a prevalent nodular pattern. In one patient, the contrast enhancement pattern differed between the two modalities: Contrast-enhanced US showed enhancement of the entire bulk of the tumor, whereas gadolinium-enhanced T1-weighted MR imaging demonstrated peripheral contrast enhancement. Conclusion Glioblastoma contrast enhancement with contrast-enhanced US is superimposable on that provided with preoperative gadolinium-enhanced T1-weighted MR imaging regarding location, margins, morphologic features, and dimensions, with a similar enhancement pattern in most cases. Thus, contrast-enhanced US is of potential use in the surgical management of GBM

Imaging the three-dimensional architecture of the Middle Aterno basin (2009 L’Aquila earthquake, Central Italy) using ground TDEM and seismic noise surveys: preliminary results

We present preliminary results from a multidisciplinary geophysical approach applied to the imaging of the threedimensional architecture of the Middle Aterno basin, close to the epicentral area of the 2009 L’Aquila earthquake (central Italy). We collected several time domain electromagnetic soundings (TDEM) coupled with seismic noise measurements focusing on the characterization of the bedrock/infill interface. Our preliminary results agree with existing geophysical data collected in the area, and show that the southeastern portion of the basin is characterized by a deepening of the Mesozoic-Tertiary bedrock down to a depth of more than 450 m. We found that a joint use of electromagnetic and seismic methods significantly contributes in obtaining new insights on the 3D geometry of the Middle Aterno basin. Moreover, we believe that our combined approach based on TDEM and noise measurements can be adopted to investigate similar geological settings elsewhere

Rilievi geologici nell’area epicentrale della sequenza sismica dell’Aquilano del 6 aprile 2009

Il 6 Aprile 2009 un terremoto di Ml=5.8 (Mw=6.2) ha colpito L’Aquila e la media valle dell’Aterno in Abruzzo. In questo lavoro presentiamo in maniera sintetica i rilievi geologici effettuati in campagna dal gruppo di lavoro EmerGeo a seguito della sequenza sismica aquilana. Le attività di rilevamento condotte sono consistite principalmente nella verifica, definizione e caratterizzazione delle deformazioni cosismiche superficiali osservate lungo le strutture tettoniche note in letteratura; sono stati inoltre rilevati e riportati altri effetti cosismici locali (fratture su asfalto, frane e scivolamenti) non direttamente collegati alla presenza di strutture tettoniche. In totale sono stati rilevati oltre 300 punti di osservazione su una porzione di territorio estesa circa 900 km2. L’analisi preliminare dei rilievi effettuati indica che le rotture osservate lungo la faglia di Paganica, per la continuità e le caratteristiche, rappresentano l’espressione superficiale della faglia responsabile dell’evento del 6 aprile 2009, e che le rotture lungo le faglie di Bazzano e di Monticchio-Fossa possono rappresentare l’espressione in superficie di una struttura antitetica riattivata durante l’evento