Supply of sulphur to S-deficient young barley seedlings restores their capability to cope with iron shortage

The effect of the S nutritional status on a plant's capability to cope with Fe shortage was studied in solution cultivation experiments in barley (Hordeum vulgare L. cv. Europa). Barley is a Strategy II plant and responds to Fe deficiency by secretion of chelating compounds, phytosiderophores (PS). All PS are derived from nicotianamine whose precursor is methionine. This suggests that a long-term supply of an inadequate amount of S could reduce a plant's capability to respond to Fe deficiency by limiting the rate of PS biosynthesis. The responses of barley (Hordeum vulgare L. cv. Europa) plants grown for 12 d on Fe-free nutrient solutions (NS) containing 0 or 1.2 mM SO42-, was examined after 24 h or 48 h from transfer to NS containing 1.2 mM SO42-. After the supply of S was restored to S-deprived plants, an increase in PS release in root exudates was evident after 24 h of growth in S-sufficient NS and the increment reached values up to 4-fold higher than the control 48 h after S resupply. When S was supplied to S-deficient plants, leaf ATPS (EC 2.7.7.4) and OASTL (EC 4.2.99.8) activities exhibited a progressive recovery. Furthermore, root HvST1 transcript abundance remained high for 48 h following S resupply and a significant increase in the level of root HvYS1 transcripts was also found after only 24 h of S resupply. Data support the idea that the extent to which the plant is able to cope with Fe starvation is strongly associated with its S nutritional status. In particular, our results are indicative that barley plants fully recover their capability to cope with Fe shortage after the supply of S is restored to S-deficient plants

Inhomogeneous turbulence in the vicinity of a large scale coherent vortex

We study the statistics of turbulent velocity fluctuations in the neighbourhood of a strong large scale vortex at very large Reynolds number. At each distance from the vortex core, we observe that the velocity spectrum has a power law ``inertial range'' of scales and that intermittency -- defined as the variation of the probability density function (PDF) of velocity increments as the length of the increment is varied -- is also present. We show that the spectrum scaling exponents and intermittency characteristics vary with the distance to the vortex. They are also influenced by the large scale dynamics of the vortex.Comment: submitted to europhys lett, 6 pages, 5 figure

Experimental test of the Gallavotti-Cohen fluctuation theorem in turbulent flows

We test the fluctuation theorem from measurements in turbulent flows. We study the time fluctuations of the force acting on an obstacle, and we consider two experimental situations: the case of a von K\'arm\'an swirling flow between counter-rotating disks (VK) and the case of a wind tunnel jet. We first study the symmetries implied by the Gallavotti-Cohen fluctuation theorem (FT) on the probability density distributions of the force fluctuations; we then test the Sinai scaling. We observe that in both experiments the symmetries implied by the FT are well verified, whereas the Sinai scaling is established, as expected, only for long times

Lagrangian temperature, velocity and local heat flux measurement in Rayleigh-Benard convection

We have developed a small, neutrally buoyant, wireless temperature sensor. Using a camera for optical tracking, we obtain simultaneous measurements of position and temperature of the sensor as it is carried along by the flow in Rayleigh-B\'enard convection, at $Ra \sim 10^{10}$. We report on statistics of temperature, velocity, and heat transport in turbulent thermal convection. The motion of the sensor particle exhibits dynamics close to that of Lagrangian tracers in hydrodynamic turbulence. We also quantify heat transport in plumes, revealing self-similarity and extreme variations from plume to plume.Comment: 4 page

Melanoma Cells Inhibit iNKT Cell Functions via PGE2 and IDO1

Simple Summary The unique properties of invariant natural killer T (iNKT) cells make them an attractive candidate for cancer-adoptive immunotherapy. However, despite their potential, clinical studies have not consistently shown successful outcomes. This lack of efficacy is likely attributed to the immunosuppressive nature of the tumor microenvironment. In this study, we investigated the role of melanoma cell lines in suppressing iNKT cell functions, even in the presence of their specific antigen. Additionally, we aimed to identify the key factors responsible for this immunosuppressive effect. Understanding the primary contributors to the failure of iNKT cell-based therapy is crucial for developing new treatment strategies. Invariant natural killer T (iNKT) cells are a distinct group of immune cells known for their immunoregulatory and cytotoxic activities, which are crucial in immune surveillance against tumors. They have been extensively investigated as a potential target for adoptive cell immunotherapy. Despite the initial promise of iNKT cell-based immunotherapy as a treatment for melanoma patients, its effective utilization has unfortunately yielded inconsistent outcomes. The primary cause of this failure is the immunosuppressive tumor microenvironment (TME). In this study, we specifically directed our attention towards melanoma cells, as their roles within the TME remain partially understood and require further elucidation. Methods: We conducted co-culture experiments involving melanoma cell lines and iNKT cells. Results: We demonstrated that melanoma cell lines had a significant impact on the proliferation and functions of iNKT cells. Our findings revealed that co-culture with melanoma cell lines led to a significant impairment in the expression of the NKG2D receptor and cytolytic granules in iNKT cells. Moreover, we observed a strong impairment of their cytotoxic capability induced by the presence of melanoma cells. Furthermore, through the use of selective inhibitors targeting IDO1 and COX-2, we successfully demonstrated that the melanoma cell line's ability to impair iNKT cell activation and functions was attributed to the up-regulation of IDO1 expression and PGE2 production

Intravital imaging reveals p53-dependent cancer cell death induced by phototherapy via calcium signaling.

One challenge in biology is signal transduction monitoring in a physiological context. Intravital imaging techniques are revolutionizing our understanding of tumor and host cell behaviors in the tumor environment. However, these deep tissue imaging techniques have not yet been adopted to investigate the second messenger calcium (Ca2+). In the present study, we established conditions that allow the in vivo detection of Ca2+ signaling in three-dimensional tumor masses in mouse models. By combining intravital imaging and a skinfold chamber technique, we determined the ability of photodynamic cancer therapy to induce an increase in intracellular Ca2+ concentrations and, consequently, an increase in cell death in a p53-dependent pathway

Reply to Comment on " Universal Fluctuations in Correlated Systems"

Reply to the comment, cond-mat/0209398 by by N.W. Watkins, S.C. Chapman, and G. RowlandsComment: To appear In Physical Review Letter