Dataset supporting the proteomic differences found between excretion/secretion products from two isolates of Fasciola hepatica newly excysted juveniles (NEJ) derived from different snail hosts

Here we present the proteomic profile datasets of two Fasciola hepatica NEJ isolates derived from different snail hosts: Lymnaea viatrix and Pseudosuccinea columella. The data used in the analysis are related to the article ‘A proteomic comparison of excretion/secretion products in Fasciola hepatica newly excysted juveniles (NEJ) derived from Lymnaea viatrix or Pseudosuccinea columella’ (Di Maggio et al., 2019

Across intra-mammalian stages of the liver f luke Fasciola hepatica: a proteomic study

Fasciola hepatica is the agent of fasciolosis, a foodborne zoonosis that affects livestock production and human health. Although flukicidal drugs are available, re-infection and expanding resistance to triclabendazole demand new control strategies. Understanding the molecular mechanisms underlying the complex interaction with the mammalian host could provide relevant clues, aiding the search for novel targets in diagnosis and control of fasciolosis. Parasite survival in the mammalian host is mediated by parasite compounds released during infection, known as excretory/secretory (E/S) products. E/S products are thought to protect parasites from host responses, allowing them to survive for a long period in the vertebrate host. This work provides in-depth proteomic analysis of F. hepatica intra-mammalian stages, and represents the largest number of proteins identified to date for this species. Functional classification revealed the presence of proteins involved in different biological processes, many of which represent original findings for this organism and are important for parasite survival within the host. These results could lead to a better comprehension of host-parasite relationships, and contribute to the development of drugs or vaccines against this parasite

Clustering of ingredients with amino acid composition similar to the nutritional requirement of Nile tilapia

The search for balanced diets, which may elicit improved growth of fish, requires appropriate selection of available protein sources. This study aims at clustering feedstuffs according to amino acid profile, determining which ones show essential amino acids (EAA) profiles closer to the ideal dietary amino acids requirements of Nile tilapia (Oreochromis niloticus), and studying the relationship among amino acids feedstuffs groups. Tabled data on EAA more cystine and tyrosine, in relation to lysine contents, of 40 feedstuffs ordinarily used to formulate fish diets were studied. Feedstuffs were grouped according to amino acids profile by cluster analysis of Euclidean distances. The principal components analysis was used to determine the relationship among amino acids in each feedstuff group. Three groups of ingredients were parted and two ingredients, low tannin sorghum and corn gluten meal 60%, did not go with any group. Dietary amino acids requirements of Nile tilapia were similar to the amino acid profile of 22 feedstuffs. The principal component analysis explained with three principal components more than 75% of total variance of amino acids in three feedstuff groups. Therefore, until additional, detailed information on amino acids availability of different ingredients is consolidated, total amino acids profiles will continue to be important information to select and use conventional or surrogate ingredients for formulating and processing feeds for tilapia.A busca de uma ração balanceada, que proporcione maior crescimento aos peixes, passa pela escolha adequada das fontes protéicas disponíveis. Este estudo teve por objetivo agrupar alimentos de acordo com o perfil de aminoácidos essenciais, determinando quais mostram perfis mais próximos do requerimento da tilápia do Nilo (Oreochromis niloticus), e estudar a relação entre os aminoácidos dentro dos agrupamentos obtidos. Foram utilizadas composições de aminoácidos em relação ao conteúdo de lisina, de 40 alimentos comumente utilizados como ingredientes na formulação de dietas para peixes. Os ingredientes foram agrupados de acordo com o perfil de aminoácidos utilizando a análise de agrupamento por meio da distância Euclidiana, enquanto a análise de componentes principais foi utilizada para determinar a relação entre os aminoácidos em cada grupo obtido. Três grupos de ingredientes foram formados e apenas dois ingredientes, sorgo baixo tanino e farelo de glúten de milho 60%, não entraram em nenhum dos três grupos. A exigência de aminoácidos da tilápia do Nilo foi semelhante ao perfil de aminoácidos encontrado em 22 alimentos. A análise de componentes principais conseguiu resumir e explicar 75% da variância total com apenas três componentes principais. Até que maiores informações sobre a disponibilidade de aminoácidos de diferentes ingredientes sejam obtidas, o perfil total de aminoácidos continuará a ser uma informação valiosa na escolha dos ingredientes a serem utilizados na formulação e processamento de alimentos para tilápia do Nilo

Expression profile of Rhipicephalus microplus vitellogenin receptor during oogenesis

The vitellogenin receptor (VgR), which belongs to the low-density lipoprotein receptors(LDLR) family, regulates the absorption of yolk protein accumulated in developing oocytes during oogenesis. In the present study, the full sequence of Rhipicephalus microplus VgR (RmVgR) and the partial sequence of Rhipicephalus appendiculatus VgR (RaVgR) ORF were determined and cloned. The RmVgR amino acid sequence contains the five highly conserved structural motifs characteristic of LDLR superfamily members, the same overall structure as observed in other species. Phylogenetic analysis separated VgRs in two major groups, corresponding to receptors from acarines and insects. Consistent with observations from other arthropods, RmVgR was specifically expressed in the ovarian tissue and its peak of expression occurs in females that are detaching from the host. Silencing with RmVgR dsRNA reduced VgR expression, which resulted in reduced fertility, evidenced by a decrease in the number of larvae. The present study confirms RmVgR is a specific receptor involved in yolk protein uptake and oocyte maturation in R. microplus, playing an important role in tick reproduction