82 research outputs found
Endophilin-A coordinates priming and fusion of neurosecretory vesicles via intersectin
Endophilins-A are conserved endocytic adaptors with membrane curvature-sensing and -inducing properties. We show here that, independently of their role in endocytosis, endophilin-A1 and endophilin-A2 regulate exocytosis of neurosecretory vesicles. The number and distribution of neurosecretory vesicles were not changed in chromaffin cells lacking endophilin-A, yet fast capacitance and amperometry measurements revealed reduced exocytosis, smaller vesicle pools and altered fusion kinetics. The levels and distributions of the main exocytic and endocytic factors were unchanged, and slow compensatory endocytosis was not robustly affected. Endophilin-A’s role in exocytosis is mediated through its SH3-domain, specifically via a direct interaction with intersectin-1, a coordinator of exocytic and endocytic traffic. Endophilin-A not able to bind intersectin-1, and intersectin-1 not able to bind endophilin-A, resulted in similar exocytic defects in chromaffin cells. Altogether, we report that two endocytic proteins, endophilin-A and intersectin-1, are enriched on neurosecretory vesicles and regulate exocytosis by coordinating neurosecretory vesicle priming and fusion
Mayaro Fever Virus, Brazilian Amazon
In February 2008, a Mayaro fever virus (MAYV) outbreak occurred in a settlement in Santa Barbara municipality, northern Brazil. Patients had rash, fever, and severe arthralgia lasting up to 7 days. Immunoglobulin M against MAYV was detected by ELISA in 36 persons; 3 MAYV isolates sequenced were characterized as genotype D
Teores de cálcio em dietas para codornas japonesas no terço final de postura (45 a 57 semanas de idade)
Micropropagation and in vitro conservation of Neoglaziovia variegata (Arr. Cam.) mez, a fiber producing bromeliad from Brazil
Efeito dos níveis de triptofano digestível em dietas para codornas japonesas em postura
Population biology of Macrobrachium jelskii (Miers, 1877) (Decapoda, Palaemonidae) from an artificial pond in Bahia, Brazil
Impacto do monocultivo de café sobre os indicadores biológicos do solo na zona da mata mineira
Sophora tomentosa e Crotalaria vitellina (Fabaceae): biologia reprodutiva e interações com abelhas na restinga de Ubatuba, São Paulo
Effects of gut passage on the germination of seeds ingested by didelphid marsupials in a neotropical savanna
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