Application of Impedimetric and Voltammetric Genosensor for Detection of a Biological Warfare: Anthrax

WOS: 000261876800005A strategy for the detection of anthrax, which is a potential biological weapon by using an electrochemical genosensing technology, is investigated. An alkanathiol-linked or unlabeled capture probe related to B. anthracis is immobilized onto gold or graphite electrode surface. A 101-mer anthrax target is used for hybridization. The extent of hybridization between probe and target sequences is determined by using differential pulse voltammetry (DPV) and electrochemical impedance spectrometry (EIS). EIS analysis are based on electron transfer resistance (R(ct)) in the presence of [Fe(CN)(6)](3-14-) and DPV measurements are based on transduction of both guanine oxidation and Meldola's blue (MDB) reduction signal as hybridization indicator. The response of the probe-modified electrodes which was interacted with a noncomplementary sequence was the same as the responses of probe-modified surface and proved the specifity of the hybridization with the target. According to these results the developed genosensors based on EIS and DPV techniques can be employed for rapid and selective detection of B. anthracis.TUBI-TAKTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG 107S163]; Ege University Research FoundEge University [07/ECZ/022]The authors acknowledge financial support from TUBI-TAK (Project number SBAG 107S163) and Ege University Research Found (Project No: 07/ECZ/022)

Comparison of physical examination and MRI for the diagnosis of intraarticular knee pathologies: analysis of 968 knees

BACKGROUND: It is very important to evaluate intraarticular knee pathologies carefully to make a proper diagnosis. The most important diagnostic tools are history-taking, physical examination including special knee tests, and radiology including magnetic resonance imaging. The purpose of this study is to make a comparison between physical examination and magnetic resonance imaging for intraarticular knee pathologies before knee arthroscopy which is accepted as the gold standard for these types of pathologies

Salivary Adiponectin and Leptin Levels are Increased in Women with Gestational Diabetes Mellitus and Gingival Inflammation

WOS: 000457800200009PubMed ID: 30574608Purpose: Gestational diabetes mellitus (GDM) is defined as glucose intolerance with first onset or diagnosis in pregnancy. This study evaluated clinical and biochemical parameters in a possible association between GDM and gingival inflammation. Materials and Methods: A total of 87 pregnant women - 44 with GDM and 43 without (NGDM) - were included. Subgroups were created according to gingival inflammation. Plaque index (PI), bleeding on probing (BOP), and probing depth (PD) were recorded. Results: Age, anthropometric variables and baby weight (g) were all statistically significantly higher in the GDM group (p < 0.0001). Systolic and diastolic blood pressure (mmHg), saliva, serum leptin and adiponectin levels were similar in the GDM and NGDM groups (p = 0.605, p = 0.662, p = 0.737, and p = 0.596, respectively). Salivary adiponectin levels were statistically significantly higher in the two subgroups with gingivitis compared to those with clinically healthy periodontium (p < 0.01). Serum adiponectin levels were statistically significantly higher in the NGDM subgroup with gingivitis than the NGDM group with clinically healthy periodontium (p < 0.05). Statistically significant positive correlations were found between PD, PI, BOP and saliva adiponectin levels in the GDM group (p < 0.05). Positive correlations were also found between clinical periodontal parameters and saliva, serum levels of adiponectin in the control group without GDM (p < 0.05). Conclusion: The higher salivary adiponectin levels in the gingivitis groups suggest that gingival inflammation is more likely to influence local inflammatory parameters both in the presence and absence of GDM. Further largerscale studies are required to better clarify the possible interactions between gingival inflammation and GDM.Ege University Research FoundationEge University [2013 DIS 026]This research was supported by a grant from the Ege University Research Foundation (Project No: 2013 DIS 026). The study protocol was approved by the Ethics Committee of Medical Faculty of Ege University (protocol number 13-2/9). No benefits in any form have been received or will be received from a commercial party related directly or indirectly to the subject of this article

What is the rate of tobacco usage among dental school students?

Kuru, Leyla/0000-0002-7599-2409;WOS: 000467864900002

Serum sestrin 2 levels in patients with uterine leiomyomas

Our aim was to evaluate SESN2 levels in patients with uterine leiomyomas by comparing serum SESN2 levels in myoma patients with the levels in healthy women to deepen our understanding of the pathophysiology of uterine leiomyomas. Patients 18–50 years of age who applied to the University of Health Sciences Turkey, Istanbul Kanuni Sultan Suleyman Training and Research Hospital between January and March 2021 and who were diagnosed with uterine leiomyoma were defined as the ‘myoma group’. The control group included patients without any sign of leiomyomas in routine ultrasonography. The patients’ demographic features, gynecological symptoms, myoma volume and classification were recorded. Serum SESN2 concentrations in venous blood samples were measured using a sandwich enzyme-linked immunosorbent assay (ELISA) kit. The study included 31 patients in the myoma group and 30 in the control group. The mean age/gravid/parity or BMI values did not differ significantly between the groups. The only gynecological symptom that showed a significant difference was menorrhagia. Serum SESN2 levels were significantly higher in the myoma group then the control groups (11.7 ± 2.5) (p < 0.001). In conclusion, although uterine leiomyoma is the most common benign tumour in women of reproductive age, there are no known markers for predicting the development of leiomyomas. Based on the results of the current study, SESN2 could be such a marker.IMPACT STATEMENT What is already known on this subject? Uterine leiomyoma is the most common type of benign tumour in women of reproductive age as well as the most common indication for a hysterectomy. Symptoms associated with uterine leiomyoma include abnormal bleeding, chronic pelvic pain, menorrhagia, dysmenorrhoea, dyspareunia and anaemia, which adversely affect the patient’s quality of life. Sestrins are a family of metabolic regulator proteins that play a potential role in carcinogenesis. What the results of this study add? This is the first study evaluating the role of sestrin in the development of uterine leiomyomas. Significantly higher levels of sestrin 2 (SESN2) were detected in patients with leiomyomas. What are the implications of these findings for clinical practice and/or further research? Although uterine leiomyoma is the most common type of benign tumour in women of reproductive age, there are still many unknowns regarding its pathophysiology. Further, there are still no known markers for predicting the development of leiomyomas. Hence, primary prevention is not possible. Based on the results of the current study SESN2, could be such a marker. Further studies are needed to confirm the results of this study

Spatial Growth Factor Delivery for 3D Bioprinting of Vascularized Bone with Adipose-Derived Stem/Stromal Cells as a Single Cell Source

Encapsulating multiple growth factors within a scaffold enhances the regenerative capacity of engineered bone grafts through their localization and controls the spatiotemporal release profile. In this study, we bioprinted hybrid bone grafts with an inherent built-in controlled growth factor delivery system, which would contribute to vascularized bone formation using a single stem cell source, human adipose-derived stem/stromal cells (ASCs) in vitro. The strategy was to provide precise control over the ASC-derived osteogenesis and angiogenesis at certain regions of the graft through the activity of spatially positioned microencapsulated BMP-2 and VEGF within the osteogenic and angiogenic bioink during bioprinting. The 3D-bioprinted vascularized bone grafts were cultured in a perfusion bioreactor. Results proved localized expression of osteopontin and CD31 by the ASCs, which was made possible through the localized delivery activity of the built-in delivery system. In conclusion, this approach provided a methodology for generating off-the-shelf constructs for vascularized bone regeneration and has the potential to enable single-step, in situ bioprinting procedures for creating vascularized bone implants when applied to bone defects

What is the rate of tobacco usage among dental school students?

Introduction This survey aimed to investigate the rate of students in dental schools that use various tobacco products and possible factors affecting their attitudes towards tobacco products. Methods The survey was conducted between May 1 and June 30, 2018 and participants were from ten dental schools in Turkey. First year students and fifth year students were compared in terms of tobacco product usage and their attitudes towards tobacco. Data were tested statistically using Statistical Package for Social Sciences (SPSS) 15.0 program and chi-square test. Results A total of 1228 students from eleven different dental schools completed the online survey. Majority of the participants were females (62.1 %) and males were less (37.9 %). Mean age of the participants was 21.8±2.9 years (min-max; 18-54 years). The overall rate of current smokers was 38.11%, but 56.6% of the participants have inhaled tobacco smoke. More than half (55.3%) of the current smokers stated that they wish to quit and 36.7% of these students have attempted to quit within the last six months. More than 10% of all participants defined themselves as highly addicted to cigarette smoking. The rate of waterpipe smokers were 41.2% and 83.7% of these students were not willing to stop waterpipe smoking. Conclusions The rate of current tobacco smokers is rather high among dental school students and this finding points out the need for more effective precautions against tobacco. Funding This study has been funded solely by the institutions of the authors