The Enid Echo

Weekly newspaper ("A Farmer's Journal") from Enid, Oklahoma Territory that includes local, territorial, and national news along with advertising

Staphylococcus cohnii spp urealyticus: case report on an uncommon pathogen

Coagulase-negative Staphylococcus has emerged as an important agent in nosocomial infections. In this study, we report a case of bacteremia associated with a central venous catheter, caused by Staphylococcus cohnii spp urealyticus that was isolated in blood cultures from a 53-year-old male patient who was admitted to a general hospital in the city of São Paulo. We discuss in this report the difficulty in routinely identifying this microorganism in the clinical microbiology laboratory. Staphylococcus cohnii spp urealyticus is a microorganism found in human skin as part of the normal microbiota, and it can cause serious infections in humans, in some situations.Staphylococcus coagulase negativos tem surgido como importantes agentes em infecções de pacientes hospitalizados. Neste estudo, relatamos o caso de bacteremia associada a cateter venoso central devido a Staphylococcus cohnii spp urealyticus isolado em hemocultura de um paciente do sexo masculino, 53 anos, internado em hospital geral da cidade de São Paulo. Discutimos nesse relato a dificuldade em identificar rotineiramente esse microrganismo no Laboratório de Microbiologia Clínica. Staphylococcus cohnii spp urealyticus é um microrganismo encontrado na pele dos seres humanos como parte da microbiota normal, podendo em algumas situações causar sérias infecções em humanos

Comparative phenotypic and genomic features of Staphylococci from sonication fluid of orthopedic implant-associated infections with poor outcome

Staphylococcus spp. remain the leading biofilm-forming agents causing orthopedic im-plant-associated infections (OIAI). This is a descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and coagulase-negative Staphylococcus (CoNS) recovered from OIAIs patients that progressed to treatment failure. Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibi-otic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS). All S. aureus harbored mecA, blaZ, and multiple resistance genes for aminogly-cosides and quinolones. All MRSA were strong biofilm producers harboring the complete icaADBC and icaR operon. Seven CoNS isolates comprising five species (S. epidermidis, S. haemolyticus, S. sci-uri, S. capitis and S. lugdunensis) were analyzed, with mecA gene detected in five isolates. S. haemoli-tycus (isolate 95), and S. lugdunensis were unable to form biofilm and did not harbor the complete icaADBCR operon. High variability of adhesion genes was detected, with atl, ebp, icaADBC operon, and IS256 being the most common. In conclusion, MRSA and CoNS isolates carrying genes for bio-film production, and resistance to β-lactam and aminoglycosides are associated with treatment fail-ure in OIAIs

First case report of non-human primates (Alouatta clamitans) with the hypervirulent Klebsiella pneumoniae serotype K1 strain ST 23: A possible emerging wildlife pathogen

BackgroundHypervirulent strain of Klebsiella pneumoniae genotype K1 isolates have recently emerged, causing severe pyogenic liver abscess complicated by devastating metastatic infections in humans. MethodsWe describe a short outbreak of the non-human primate (NHP) research center, associated with a hypervirulent K.pneumoniae. The genetic similarity of the strains was evaluated by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) techniques, and virulence encoding genes were detected by polymerase chain reaction (PCR). ResultsThe isolates were phenotypically like strains causing community-acquired invasive liver abscess syndrome in humans. All strains exhibited identical PFGE patterns and were found to belong to ST23 and presented a hypermucovisity phenotype and possessed magA and rmpA gene. ConclusionThis is the first case report of NHPs caused by K.pneumoniae displaying a hypermucoviscosity phenotype and belonging to capsular serotypes K1 and ST23.Indaial Biological Research Center-CEPESBILaboratorio Especial de Microbiologia Clinica (LEMC), Escola Paulista de Medicina, Universidade Federal de Sao Paulo, UNIFESPUniv Reg Blumenau FURB, Dept Med Vet, Blumenau, SC, BrazilUniv Reg Blumenau FURB, Dept Ciencias Naturais, Blumenau, SC, BrazilUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, LEMC, Sao Paulo, BrazilUniv Reg Blumenau FURB, Dept Ciencias Farmaceut, Blumenau, SC, BrazilUniv Fed Sao Paulo UNIFESP, Escola Paulista Med, LEMC, Sao Paulo, BrazilWeb of Scienc