Debris flow susceptibility mapping using the Rock Engineering System (RES) method: a case study

The main purpose of the present study is to develop a debris flow susceptibility map of a mountain area (Susa Valley, Western Italian Alps) by using an upgraded version of the Bonetto et al. (Journal of Mountain Science 18, 2021) approach based on the Rock Engineering System (RES) method. In particular, the area under investigation was discretized in a 5 × 5-m grid on which GIS based analyses were performed. Starting from available databases, several geological, geo-structural, morphological and hydrographical predisposing parameters were identified and codified into two interaction matrices (one for outcropping lithologies and one for Quaternary deposits), to evaluate their mutual interactions and their weight in the susceptibility estimation. The result for each grid point is the debris flow propensity index (DfPI), an index that estimates the susceptibility of the cell to be a potential debris flow source. The debris flow susceptibility map obtained was compared with those obtained from two expedited and universally recognized susceptibility methods, i.e. the Regional Qualitative Heuristic Susceptibility Mapping (RQHSM) and the Likelihood Ratio (LR). Each map was validated by using the Prediction Rate Curve method. The limitations and strong points of the approaches analysed are discussed, with a focus on the innovativeness and uniqueness of the RES. In fact, in the study site, the RES method was the most efficient for the detection of potential source areas. These results prove its robustness, cost-effectiveness and speed of application in the identification and mapping of sectors capable of triggering debris flow

The Endocannabinoid System in the Mediterranean Mussel Mytilus galloprovincialis: Possible Mediators of the Immune Activity?

Anandamide (AEA) is one of the best characterized members of the endocannabinoid family and its involvement in many pathophysiological processes has been well documented in vertebrates and invertebrates. Here, we report the biochemical and functional characterization of key elements of the endocannabinoid system in hemocytes isolated from the Mediterranean mussel Mytilus galloprovincialis. We also show the effects of exogenous AEA, as well as of capsaicin, on the cell ability to migrate and to activate the respiratory burst, upon in vitro stimulation of phagocytosis. Interestingly, our findings show that both AEA and capsaicin suppress the hemocyte response and that the use of selective antagonists of CB2 and TRPV1 receptors revert their inhibitory effects. Overall, present data support previous evidence on the presence of endocannabinoid signaling in mollusks and advance our knowledge about the evolutionary origins of this endogenous system and its role in the innate response of mollusks

Genetic diversity of Arcobacter isolated from bivalves of Adriatic and their interactions with Mytilus galloprovincialis hemocytes

The human food-borne pathogens Arcobacter butzleri and A. cryaerophilus have been frequently isolated from the intestinal tracts and fecal samples of different farm animals and, after excretion, these microorganisms can contaminate the environment, including the aquatic one. In this regard, A. butzleri and A. cryaerophilus have been detected in seawater and bivalves of coastal areas which are affected by fecal contamination. The capability of bivalve hemocytes to interact with bacteria has been proposed as the main factor inversely conditioning their persistence in the bivalve. In this study, 12 strains of Arcobacter spp. were isolated between January and May 2013 from bivalves of Central Adriatic Sea of Italy in order to examine their genetic diversity as well as in vitro interactions with bivalve components of the immune response, such as hemocytes. Of these, seven isolates were A. butzleri and five A. cryaerophilus, and were genetically different. All strains showed ability to induce spreading and respiratory burst of Mytilus galloprovincialis hemocytes. Overall, our data demonstrate the high genetic diversity of these microorganisms circulating in the marine study area. Moreover, the Arcobacter–bivalve interaction suggests that they do not have a potential to persist in the tissues of M. galloprovincialis