Deeply subducted continental fragments - Part 2: Insight from petrochronology in the central Sesia Zone (western Italian Alps)

Subducted continental terranes commonly comprise an assembly of subunits that reflect the different tectono-metamorphic histories they experienced in the subduction zone. Our challenge is to unravel how, when, and in which part of the subduction zone these subunits were juxtaposed. Petrochronology offers powerful tools to decipher pressure–temperature–time (P–T–t) histories of metamorphic rocks that preserve a record of several stages of transformation. A major issue is that the driving forces for re-equilibration at high pressure are not well understood. For example, continental granulite terrains subducted to mantle depths frequently show only partial and localized eclogitization. The Sesia Zone (NW Italy) is exceptional because it comprises several continental subunits in which eclogitic rocks predominate and high-pressure (HP) assemblages almost completely replaced the Permian granulite protoliths. This field-based study comprises both main complexes of the Sesia terrane, covering some of the recently recognized tectonic subunits involved in its assembly; hence our data constrain the HP tectonics that formed the Sesia Zone. We used a petrochronological approach consisting of petrographic and microstructural analysis linked with thermodynamic modelling and U–Th–Pb age dating to reconstruct the P–T–t trajectories of these tectonic subunits. Our study documents when and under what conditions re-equilibration took place. Results constrain the main stages of mineral growth and deformation, associated with fluid influx that occurred in the subduction channel. In the Internal Complex (IC), pulses of fluid percolated at eclogite facies conditions between 77 and 55 Ma with the HP conditions reaching  ∼  2 GPa and 600–670 °C. By contrast, the External Complex (EC) records a lower pressure peak of  ∼  0.8 GPa for 500 °C at  ∼  63 Ma. The juxtaposition of the two complexes occurred during exhumation, probably at  ∼  0.8 GPa and 350 °C; the timing is constrained between 46 and 38 Ma. Mean vertical exhumation velocities are constrained between 0.9 and 5.1 mm year−1 for the IC, up to its juxtaposition with the EC. Exhumation to the surface occurred before 32 Ma, as constrained by the overlying Biella Volcanic Suite, at a mean vertical velocity between 1.6 and 4 mm year−1. These findings constrain the processes responsible for the assembly and exhumation of HP continental subunits, thus adding to our understanding of how continental terranes behave during subduction

The sphaleron energy for SU(2)-Higgs from cooling

The cooling algorithm for saddle points presented in ref. [1] is generalized to obtain static classical solutions of the SU(2)-Higgs field theory in the limit of infinite Higgs self-coupling. The sphaleron energy obtained via this algorithm is ${\cal E}_{\rm sph}= 5.08(7)\,M_W/\alpha_W$ to be compared with 5.0707 obtained in the variational approach [2].Comment: 3 pages, uuencoded compressed PostScript file. Contribution to Lattice '94, 27 Sep - 1 Oct 1994, Bielefeld, German

Mirroring Pain in the Brain: Emotional Expression versus Motor Imitation

Perception of pain in others via facial expressions has been shown to involve brain areas responsive to self-pain, biological motion, as well as both performed and observed motor actions. Here, we investigated the involvement of these different regions during emotional and motor mirroring of pain expressions using a two-task paradigm, and including both observation and execution of the expressions. BOLD responses were measured as subjects watched video clips showing different intensities of pain expression and, after a variable delay, either expressed the amount of pain they perceived in the clips (pain task), or imitated the facial movements (movement task). In the pain task condition, pain coding involved overlapping activation across observation and execution in the anterior cingulate cortex, supplementary motor area, inferior frontal gyrus/anterior insula, and the inferior parietal lobule, and a pain-related increase (pain vs. neutral) in the anterior cingulate cortex/supplementary motor area, the right inferior frontal gyrus, and the postcentral gyrus. The 'mirroring' response was stronger in the inferior frontal gyrus and middle temporal gyrus/superior temporal sulcus during the pain task, and stronger in the inferior parietal lobule in the movement task. These results strongly suggest that while motor mirroring may contribute to the perception of pain expressions in others, interpreting these expressions in terms of pain content draws more heavily on networks involved in the perception of affective meaning

Brain mechanisms associated with facial encoding of affective states

Affective states are typically accompanied by facial expressions, but these behavioral manifestations are highly variable. Even highly arousing and negative valent experiences, such as pain, show great instability in facial affect encoding. The present study investigated which neural mechanisms are associated with variations in facial affect encoding by focusing on facial encoding of sustained pain experiences. Facial expressions, pain ratings, and brain activity (BOLD-fMRI) during tonic heat pain were recorded in 27 healthy participants. We analyzed facial expressions by using the Facial Action Coding System (FACS) and examined brain activations during epochs of painful stimulation that were accompanied by facial expressions of pain. Epochs of facial expressions of pain were coupled with activity increase in motor areas (M1, premotor and SMA) as well as in areas involved in nociceptive processing, including primary and secondary somatosensory cortex, posterior and anterior insula, and the anterior part of the mid-cingulate cortex. In contrast, prefrontal structures (ventrolateral and medial prefrontal) were less activated during incidences of facial expressions, consistent with a role in down-regulating facial displays. These results indicate that incidences of facial encoding of pain reflect activity within nociceptive pathways interacting or possibly competing with prefrontal inhibitory systems that gate the level of expressiveness

Elevated ratio of MMP2/MMP9 activity is associated with poor response to chemotherapy in osteosarcoma

Background: Matrix metalloproteinases (MMPs) are crucially involved in the regulation of multiple stages of cancer progression. Elevated MMP levels have been associated with the development of metastases and poor prognosis in several types of cancer. However, the role of MMPs in osteosarcoma and their prognostic value is still unclear. Available data are conflicting, most likely due to different technical approaches. We hypothesized that in contrast to total mRNA or protein levels frequently analyzed in previous studies the enzymatic activities of MMPs and their inhibitors the tissue inhibitors of matrix metalloproteinases (TIMPs) are closer related to their biological functions. We therefore aimed to evaluate the reliability of different zymography techniques for the quantification of MMP and TIMP activities in osteosarcoma biopsies in order to investigate their distribution, possible regulation and prognostic value. Methods: All analyses were done using cryo-conserved osteosarcoma pretreatment biopsies (n = 18). Gene and protein expression of MMPs and TIMPs were analyzed by RT-qPCR and western blot analysis, respectively. Overall MMP activity was analyzed by in situ zymography, individual MMP activities were analyzed by gelatin zymography. Reverse zymography was used to detect and quantify TIMP activities. Results: Strong overall MMP activities could be detected in osteosarcoma pretreatment biopsies with MMP2 and MMP9 as predominant active MMPs. In contrast to total RNA or protein expression MMP2 and MMP9 activities showed significant quantitative differences between good and poor responders. While MMP9 activity was high in the good responder group and significantly decreased in the poor responder group, MMP2 activity showed a reverse distribution. Likewise, significant differences were detected concerning the activity of TIMPs resulting in a negative correlation of TIMP1 activity with MMP2 activity (p = 0.044) and negative correlations of TIMP2 and TIMP3 with MMP9 activity (p = 0.007 and p = 0.006). Conclusion: In contrast to mRNA or protein levels MMP and TIMP activities showed significant differences between the analyzed good and poor responder groups. A shift from MMP9 to predominant MMP2 activity is associated with poor response to chemotherapy suggesting that the ratio of MMP2/MMP9 activity might be a valuable and easily accessible marker to predict the response to chemotherapy in osteosarcoma

Turbulent regimes in collisions of 3D Alfv\'en-wave packets

Using 3D gyrofluid simulations, we revisit the problem of Alfven-wave (AW) collisions as building blocks of the Alfvenic cascade and their interplay with magnetic reconnection at magnetohydrodynamic (MHD) scales. Depending on the large-scale nonlinearity parameter $\chi_0$ (the ratio between AW linear propagation time and nonlinear turnover time), different regimes are observed. For strong nonlinearities ($\chi_0\sim1$), turbulence is consistent with a dynamically aligned, critically balanced cascade--fluctuations exhibit a scale-dependent alignment $\sin\theta_k\propto k_\perp^{-1/4}$, a $k_\perp^{-3/2}$ spectrum and $k_\|\propto k_\perp^{1/2}$ spectral anisotropy. At weaker nonlinearities (small $\chi_0$), a spectral break marking the transition between a large-scale weak regime and a small-scale $k_\perp^{-11/5}$ tearing-mediated range emerges, implying that dynamic alignment occurs also for weak nonlinearities. At $\chi_0<1$ the alignment angle $\theta_{k_\perp}$ shows a stronger scale dependence than in the $\chi_0\sim1$ regime, i.e. $\sin\theta_k\propto k_\perp^{-1/2}$ at $\chi_0\sim0.5$, and $\sin\theta_k\propto k_\perp^{-1}$ at $\chi_0\sim0.1$. Dynamic alignment in the weak regime also modifies the large-scale spectrum, scaling roughly as $k_\perp^{-3/2}$ for $\chi_0\sim0.5$ and as $k_\perp^{-1}$ for $\chi_0\sim0.1$. A phenomenological theory of dynamically aligned turbulence at weak nonlinearities that can explain these spectra and the transition to the tearing-mediated regime is provided; at small $\chi_0$, the strong scale dependence of the alignment angle combines with the increased lifetime of turbulent eddies to allow tearing to onset and mediate the cascade at scales that can be larger than those predicted for a critically balanced cascade by several orders of magnitude. Such a transition to tearing-mediated turbulence may even supplant the usual weak-to-strong transition.Comment: 13 pages, 5 figures, 1 table; accepted for publication in The Astrophysical Journa

The electroweak sphaleron on the lattice

We study the properties of the electroweak sphaleron on a finite lattice. The cooling algorithm for saddle points is used to obtain the static classical solutions of the SU(2)-Higgs field theory. Results are presented for $M_H=\infty, M_W, 0.75M_W$. After performing finite size scaling we find good agreement with the results obtained from variational approaches. Of relevance for numerical determinations of the transition rate is that the lattice artefacts are surprisingly small for $M_W\approx M_H$.Comment: 13p LaTeX with 3 figs, uufiles -gz forma

Improvement of the Solubilization and Extraction of Curcumin in an Edible Ternary Solvent Mixture

A water-free, ternary solvent mixture consisting of a natural deep eutectic solvent (NADES), ethanol, and triacetin was investigated concerning its ability to dissolve and extract curcumin from Curcuma longa L. To this purpose, 11 NADES based on choline chloride, acetylcholine, and proline were screened using UV–vis measurements. A ternary phase diagram with a particularly promising NADES, based on choline chloride and levulinic acid was recorded and the solubility domains of the monophasic region were examined and correlated with the system’s structuring via light scattering experiments. At the optimum composition, close to the critical point, the solubility of curcumin could be enhanced by a factor of >1.5 with respect to acetone. In extraction experiments, conducted at the points of highest solubility and evaluated via HPLC, a total yield of ~84% curcuminoids per rhizome could be reached. Through multiple extraction cycles, reusing the extraction solvent, an enrichment of curcuminoids could be achieved while altering the solution. When counteracting the solvent change, even higher concentrated extracts can be obtained