3,498 research outputs found
Abnormally high content of free glucosamine residues identified in a preparation of commercially available porcine intestinal heparan sulfate
Heparan sulfate (HS) polysaccharides are ubiquitous in animal tissues as components of proteoglycans, and they participate in many important biological processes. HS carbohydrate chains are complex and can contain rare structural components such as N-unsubstituted glucosamine (GlcN). Commercially available HS preparations have been invaluable in many types of research activities. In the course of preparing microarrays to include probes derived from HS oligosaccharides, we found an unusually high content of GlcN residue in a recently purchased batch of porcine intestinal mucosal HS. Composition and sequence analysis by mass spectrometry of the oligosaccharides obtained after heparin lyase III digestion of the polysaccharide indicated two and three GlcN in the tetrasaccharide and hexasaccharide fractions, respectively. (1)H NMR of the intact polysaccharide showed that this unusual batch differed strikingly from other HS preparations obtained from bovine kidney and porcine intestine. The very high content of GlcN (30%) and low content of GlcNAc (4.2%) determined by disaccharide composition analysis indicated that N-deacetylation and/or N-desulfation may have taken place. HS is widely used by the scientific community to investigate HS structures and activities. Great care has to be taken in drawing conclusions from investigations of structural features of HS and specificities of HS interaction with proteins when commercial HS is used without further analysis. Pending the availability of a validated commercial HS reference preparation, our data may be useful to members of the scientific community who have used the present preparation in their studies
Divergence in Dialogue
Copyright: 2014 Healey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.This work was supported by the Economic and Social Research Council (ESRC; http://www.esrc.ac.uk/) through the DynDial project (Dynamics of Conversational Dialogue, RES-062-23-0962) and the Engineering and Physical Sciences Research Council (EPSRC; http://www.epsrc.ac.uk/) through the RISER
project (Robust Incremental Semantic Resources for Dialogue, EP/J010383/1). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
Spitzer Observations of Low Luminosity Isolated and Low Surface Brightness Galaxies
We examine the infrared properties of five low surface brightness galaxies
(LSBGs) and compare them with related but higher surface brightness galaxies,
using Spitzer Space Telescope images and spectra. All the LSBGs are detected in
the 3.6 and 4.5um bands, representing the stellar population. All but one are
detected at 5.8 and 8.0um, revealing emission from hot dust and aromatic
molecules, though many are faint or point-like at these wavelengths. Detections
of LSBGs at the far-infrared wavelengths, 24, 70, and 160um, are varied in
morphology and brightness, with only two detections at 160um, resulting in
highly varied spectral energy distributions. Consistent with previous
expectations for these galaxies, we find that detectable dust components exist
for only some LSBGs, with the strength of dust emission dependent on the
existence of bright star forming regions. However, the far-infrared emission
may be relatively weak compared with normal star-forming galaxies.Comment: 20 pages, 8 figures, accepted to Ap
Radial HI Profiles at the Periphery of Galactic Disks: The Role of Ionizing Background Radiation
Observations of neutral hydrogen in spiral galaxies reveal a sharp cutoff in
the radial density profile at some distance from the center. Using 22 galaxies
with known HI distributions as an example, we discuss the question of whether
this effect can be associated exclusively with external ionizing radiation, as
is commonly assumed. We show that before the surface density reaches
(the same for
galaxies of different types), it is hard to expect the gas to be fully ionized
by background radiation. For two of 13 galaxies with a sharp drop in the HI
profile, the "steepening" can actually be caused by ionization. At the same
time, for the remaining galaxies, the observed cutoff in the radial HI profile
is closer to the center than if it was a consequence of ionization by
background radiation and, therefore, it should be caused by other factors.Comment: 15 pages, 6 figure
Defining the glycosaminoglycan interactions of complement factor H-related protein 5
Complement activation is an important mediator of kidney injury in glomerulonephritis. Complement factor H (FH) and FH-related protein 5 (FHR-5) influence complement activation in C3 glomerulopathy and IgA nephropathy by differentially regulating glomerular complement. FH is a negative regulator of complement C3 activation. Conversely, FHR-5 in vitro promotes C3 activation either directly or by competing with FH for binding to complement C3b. The FH-C3b interaction is enhanced by surface glycosaminoglycans (GAGs) and the FH-GAG interaction is well-characterized. In contrast, the contributions of carbohydrates to the interaction of FHR-5 and C3b are unknown. Using plate-based and microarray technologies we demonstrate that FHR-5 interacts with sulfated GAGs and that this interaction is influenced by the pattern and degree of GAG sulfation. The FHR-5-GAG interaction that we identified has functional relevance as we could show that the ability of FHR-5 to prevent binding of FH to surface C3b is enhanced by surface kidney heparan sulfate. Our findings are important in understanding the molecular basis of the binding of FHR-5 to glomerular complement and the role of FHR-5 in complement-mediated glomerular disease
A Far Ultraviolet Study of the Old Nova V841 Oph
We have carried out a synthetic spectral analysis of archival IUE spectra of
the old nova V841 Oph (Nova Oph 1848) taken 15 years apart. The spectra reveal
a rising continuum shortward of 1560\AA, a C {\sc iv} P-Cygni profile
indicative of wind outflow associated with disk accretion in one spectrum, a
deep Ly profile, and strong N {\sc v} (1238\AA, 1242\AA) and O {\sc v}
(1371\AA) wind/coronal absorption lines. Numerous sharp interstellar resonance
lines are also present. A grid of high gravity atmospheres and accretion disk
models, spanning a wide range of inclinations, accretion rates and white dwarf
masses was compared to the de-reddened spectra. We find that, for a steady
state accretion disk model to account for the FUV spectra, the accretion rate
is only 3 10 M_{\sun}/yr, 147 years after its outburst
in 1848, with an implied distance within 300 pc. The accretion rate at
147 years post-outburst is smaller than expected for an old nova.Comment: 6 b/w figures, 2 tables, preprint accepted in the November 2005 issue
of PAS
Pushing the limit of instrument capabilities
Chemically Peculiar (CP) stars have been subject of systematic research since
more than 50 years. With the discovery of pulsation of some of the cool CP
stars, the availability of advanced spectropolarimetric instrumentation and
high signal- to-noise, high resolution spectroscopy, a new era of CP star
research emerged about 20 years ago. Together with the success in ground-based
observations, new space projects are developed that will greatly benefit for
future investigations of these unique objects. In this contribution we will
give an overview of some interesting results obtained recently from
ground-based observations and discuss on future outstanding Gaia space mission
and its impact on CP star research.Comment: Joint Discussion 04, Secsion 1, To appear in Highlights of Astronomy,
Proc. of the XXVIIth IAU General Assembly, Rio de Janeiro, Brazil, August
2009, 9 page
Symbolic Software for the Painleve Test of Nonlinear Ordinary and Partial Differential Equations
The automation of the traditional Painleve test in Mathematica is discussed.
The package PainleveTest.m allows for the testing of polynomial systems of
ordinary and partial differential equations which may be parameterized by
arbitrary functions (or constants). Except where limited by memory, there is no
restriction on the number of independent or dependent variables. The package is
quite robust in determining all the possible dominant behaviors of the Laurent
series solutions of the differential equation. The omission of valid dominant
behaviors is a common problem in many implementations of the Painleve test, and
these omissions often lead to erroneous results. Finally, our package is
compared with the other available implementations of the Painleve test.Comment: Published in the Journal of Nonlinear Mathematical Physics
(http://www.sm.luth.se/math/JNMP/), vol. 13(1), pp. 90-110 (Feb. 2006). The
software can be downloaded at either http://www.douglasbaldwin.com or
http://www.mines.edu/fs_home/wherema
A quantitative evaluation of aerosol generation during supraglottic airway insertion and removal
- …