The Examination of non-pharmacologic treatment of pain after spinal cord injury

Background: One of the primary debilitating secondary health conditions experienced by individuals living with a spinal cord injury (SCI) is chronic pain. Approximately, over 30% of SCI patients endure chronic pain after sustaining the injury, and engage in opioid pharmacotherapy as the first form of treatment. The increase in use and misuse of prescribed opioids for chronic pain can lead to both physical and psychological health risks. This danger is exacerbated by the notion that a large percentage of the SCI population have a pre-existing condition of drug and alcohol abuse and addiction. This study will examine how pain is actually being addressed through non-pharmaceutical methods among the SCI adult population.  Experimental Design: Employing a cross-sectional web survey design with a convenience sample of adults with a SCI. A Qualtrics survey is currently being administered to known individuals with SCIs via email. The survey questions will illuminate what pain management strategies are being implemented in the SCI population within the US.   Anticipated Results: The results from the data will provide insight on what non-pharmacological interventions can be employed in place of or in combination with the pharmacological management. This will allow community-based rehabilitation therapies to incorporate appropriate pain management strategies.   Potential Impact: Non-pharmacological interventions may hold the answer in treating chronic pain in the SCI population by limiting or eliminating the use of opioids. This study will help develop the most appropriate non-pharmacological intervention to test in quasi-experimental clinical trials, and ultimately minimize the use of opioids for chronic pain

LGBTQ+ College Students’ Willingness to Access Campus Health Services at a Midsized, Urban Christian University: A Study on Disparities

Introduction LGBTQ+ college students face inequitable health outcomes in both mental health and physical health versus non-LGBTQ+ counterparts (The Trevor Project, 2023), (Brittain et Dinger, 2015). LGBTQ+ students experience higher unwillingness and disparities in care access due to negative perceptions of and experiences with UHS providers (Hood et al, 2019). Research indicates that better provider LGBTQ+ cultural awareness contributes to a more affirming space which in turn benefits LGBTQ+ healthcare (Parmenter et al, 2020), (Prasad et al, 2023). Methods A 2024 cross-sectional study assessed the comfort levels of LGBTQ+ college students in accessing student health services, explore how affirming healthcare providers might encourage care, and examine perceptions of campus and home environments. Questions were derived from Hood et al (2019) and consisted of three sections examining: (1) experiences with campus resources, (2) experiences with student health services, and (3) perceptions of campus and home climate. Participants responded using a 5-point Likert scale. Data was analyzed using Excel to identify trends and overall perceptions. Results A total of 34 responses met the inclusion criteria. Respondents perceived Counseling Services as more effective in raising awareness than the Clinic or Pharmacy. Most felt that campus nondiscrimination policies and healthcare resources encouraged them to seek care. Those who disclosed their LGBTQ+ identity to providers felt more comfortable than those who did not, though most who shared their identity remained neutral on whether their provider was affirming. More respondents disagreed than agreed that their provider was affirming. Most believed affirming providers at Belmont would encourage care, while non-affirming providers would discourage it. Regarding Belmont’s healthcare providers, 41% were neutral on whether they created an affirming space, 34% agreed, and 24% disagreed. No instances of discrimination from Belmont providers were reported. Overall, most respondents viewed Belmont as an affirming environment where they felt more comfortable than at home. While 45% felt more comfortable seeking care at Belmont than at home, 19% somewhat disagreed. Additionally, 18% reported experiencing discrimination when seeking healthcare at home, which discouraged further care-seeking. Discussion This study illustrated that LGBTQ+ Belmont students feel comfortable accessing care though there is room for improvement. While most respondents agree that Belmont is an affirming environment, the same sentiment is not translated to campus health providers. Further research is needed to identify strategies to increase a positive perception of health services and providers to encourage care. Findings align with previous literature indicating that LGBTQ+ students face barriers in care and that affirming environments, providers, and resources encourage and improve outcomes. References Brittain, D.R. et Dinger, M.K. (2015). An examination of health inequities among college students by sexual orientation and sex. Journal of Public Health, 4(414). doi:https://doi.org/10.4081/jphr.2015.414 Hood, L. et al. (2019). LGBTQ College Students’ Experiences With University Health Services: An Exploratory Study. Journal of Homosexuality, 66(6), 797-814. doi:10.1080/00918369.2018.1484234 Parmenter, J.G. et al. (2020). An Exploration of LGBTQ+ Community Members\u27 Perceived Positive Perceptions of LGBTQ+ Culture. The Counseling Psychologist, 48(7), 1016-1047. doi:https://doi.org/10.1177/0011000020933188 Prasad, S. et al. (2023). Inclusive LGBTQIA+ healthcare: An interprofessional case-based experience for cultural competency awareness. Frontiers in Public Health. doi:https://doi.org/10.3389/fpubh.2022.993461 The Trevor Project. (2023). 2023 U.S. National Survey on the Mental Health of LGBTQ Young People. The Trevor Project. Retrieved September 2024, from https://www.thetrevorproject.org/survey-2023

Geniculo-Cortical Projection Diversity Revealed within the Mouse Visual Thalamus

This is the final version of the article. It was first available from PLOS via http://dx.doi.org/10.1371/journal.pone.0144846All dLGN cell co-ordinates, V1 injection sites, dLGN boundary coordinates, experimental protocols and analysis scripts are available for download from figshare at https://figshare.com/s/36c6d937b1844eec80a1.The mouse dorsal lateral geniculate nucleus (dLGN) is an intermediary between retina and primary visual cortex (V1). Recent investigations are beginning to reveal regional complexity in mouse dLGN. Using local injections of retrograde tracers into V1 of adult and neonatal mice, we examined the developing organisation of geniculate projection columns: the population of dLGN-V1 projection neurons that converge in cortex. Serial sectioning of the dLGN enabled the distribution of labelled projection neurons to be reconstructed and collated within a common standardised space. This enabled us to determine: the organisation of cells within the dLGN-V1 projection columns; their internal organisation (topology); and their order relative to V1 (topography). Here, we report parameters of projection columns that are highly variable in young animals and refined in the adult, exhibiting profiles consistent with shell and core zones of the dLGN. Additionally, such profiles are disrupted in adult animals with reduced correlated spontaneous activity during development. Assessing the variability between groups with partial least squares regression suggests that 4?6 cryptic lamina may exist along the length of the projection column. Our findings further spotlight the diversity of the mouse dLGN?an increasingly important model system for understanding the pre-cortical organisation and processing of visual information. Furthermore, our approach of using standardised spaces and pooling information across many animals will enhance future functional studies of the dLGN.Funding was provided by a Wellcome Trust grant jointly awarded to IDT and SJE (083205, www.wellcome.ac.uk), and by MRC PhD Studentships awarded to MNL and ACH (http://www.mrc.ac.uk/)

Annual and seasonal movements of migrating short-tailed shearwaters reflect environmental variation in sub-Arctic and Arctic waters

The marine ecosystems of the Bering Sea and adjacent southern Chukchi Sea are experiencing rapid changes due to recent reductions in sea ice. Short-tailed shearwaters Puffinus tenuirostris visit this region in huge numbers between the boreal summer and autumn during non-breeding season, and represent one of the dominant top predators. To understand the implications for this species of ongoing environmental change in the Pacific sub-Arctic and Arctic seas, we tracked the migratory movements of 19 and 24 birds in 2010 and 2011, respectively, using light-level geolocators. In both years, tracked birds occupied the western (Okhotsk Sea and Kuril Islands) and eastern (southeast Bering Sea) North Pacific from May to July. In August–September of 2010, but not 2011, a substantial proportion (68 % of the tracked individuals in 2010 compared to 38 % in 2011) moved through the Bering Strait to feed in the Chukchi Sea. Based on the correlation with oceanographic variables, the probability of shearwater occurrence was highest in waters with sea surface temperatures (SSTs) of 8–10 °C over shallow depths. Furthermore, shearwaters spent more time flying when SST was warmer than 9 °C, suggesting increased search effort for prey. We hypothesized that the northward shift in the distribution of shearwaters may have been related to temperature-driven changes in the abundance of their dominant prey, krill (Euphausiacea), as the timing of krill spawning coincides with the seasonal increase in water temperature. Our results indicate a flexible response of foraging birds to ongoing changes in the sub-Arctic and Arctic ecosystems

Demographics of extra-articular calcaneal fractures: Including a review of the literature on treatment and outcome

Introduction: Extra-articular calcaneal fractures represent 25-40% of all calcaneal fractures and an even higher percentage of up to 60% is seen in children. A disproportionately small part of the literature on calcaneal fractures involves the extra-articular type. The aim of this study was to investigate the incidence of extra-articular calcaneal fractures in a Level 1 trauma centre, define the distribution of the various types of fractures and compare patient demographics between extra- and intra-articular calcaneal fractures. In addition the literature was reviewed for the most common types of extra-articular calcaneal fractures with regard to incidence, treatment and clinical outcome. Methods: The radiological records between 2003 and 2005 were reviewed for intra- and extra-articular calcaneal fractures. Patient gender-distribution and age were compared. A literature search was conducted for the treatment of extra-articular calcaneal fractures. Results: In this 3-year study period a total of 49 patients with 50 extra-articular calcaneal fractures and 91 patients with 101 intra-articular fractures were identified. The median age for the first group was 32.7 years, and for the second group 40.3 years; P = 0.04. Male predominance was significantly less pronounced for extra-articular (63%) compared with intra-articular fractures (79%; P = 0.04). Conclusion: One-third of all calcaneal fractures are extra-articular. Significant differences exist between the intra- and extra-articular groups, in terms of lower age and male-female ratio. The literature study shows inconsistencies in treatment options, but most extra-articular fractures are well manageable conservatively

Joint spatiotemporal models to predict seabird densities at sea

Introduction: Seabirds are abundant, conspicuous members of marine ecosystems worldwide. Synthesis of distribution data compiled over time is required to address regional management issues and understand ecosystem change. Major challenges when estimating seabird densities at sea arise from variability in dispersion of the birds, sampling effort over time and space, and differences in bird detection rates associated with survey vessel type. Methods: Using a novel approach for modeling seabirds at sea, we applied joint dynamic species distribution models (JDSDM) with a vector-autoregressive spatiotemporal framework to survey data collected over nearly five decades and archived in the North Pacific Pelagic Seabird Database. We produced monthly gridded density predictions and abundance estimates for 8 species groups (77% of all birds observed) within Cook Inlet, Alaska. JDSDMs included habitat covariates to inform density predictions in unsampled areas and accounted for changes in observed densities due to differing survey methods and decadal-scale variation in ocean conditions. Results: The best fit model provided a high level of explanatory power (86% of deviance explained). Abundance estimates were reasonably precise, and consistent with limited historical studies. Modeled densities identified seasonal variability in abundance with peak numbers of all species groups in July or August. Seabirds were largely absent from the study region in either fall (e.g., murrelets) or spring (e.g., puffins) months, or both periods (shearwaters). Discussion: Our results indicated that pelagic shearwaters (Ardenna spp.) and tufted puffin (Fratercula cirrhata) have declined over the past four decades and these taxa warrant further investigation into underlying mechanisms explaining these trends. JDSDMs provide a useful tool to estimate seabird distribution and seasonal trends that will facilitate risk assessments and planning in areas affected by human activities such as oil and gas development, shipping, and offshore wind and renewable energy

Cost-Effectiveness Analysis of Efforts to Reduce Risk of Type 2 Diabetes and Cardiovascular Disease in Southwestern Pennsylvania, 2005-2007

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938403/pdf/PCD75A109.pd

Profiles and Majority Voting-Based Ensemble Method for Protein Secondary Structure Prediction

Machine learning techniques have been widely applied to solve the problem of predicting protein secondary structure from the amino acid sequence. They have gained substantial success in this research area. Many methods have been used including k-Nearest Neighbors (k-NNs), Hidden Markov Models (HMMs), Artificial Neural Networks (ANNs) and Support Vector Machines (SVMs), which have attracted attention recently. Today, the main goal remains to improve the prediction quality of the secondary structure elements. The prediction accuracy has been continuously improved over the years, especially by using hybrid or ensemble methods and incorporating evolutionary information in the form of profiles extracted from alignments of multiple homologous sequences. In this paper, we investigate how best to combine k-NNs, ANNs and Multi-class SVMs (M-SVMs) to improve secondary structure prediction of globular proteins. An ensemble method which combines the outputs of two feed-forward ANNs, k-NN and three M-SVM classifiers has been applied. Ensemble members are combined using two variants of majority voting rule. An heuristic based filter has also been applied to refine the prediction. To investigate how much improvement the general ensemble method can give rather than the individual classifiers that make up the ensemble, we have experimented with the proposed system on the two widely used benchmark datasets RS126 and CB513 using cross-validation tests by including PSI-BLAST position-specific scoring matrix (PSSM) profiles as inputs. The experimental results reveal that the proposed system yields significant performance gains when compared with the best individual classifier

The Phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P 3 ) Binder Rasa3 Regulates Phosphoinositide 3-kinase (PI3K)-dependent Integrin α IIb β 3 Outside-in Signaling

The class I PI3K family of lipid kinases plays an important role in integrin αIIbβ3 function, thereby supporting thrombus growth and consolidation. Here, we identify Ras/Rap1GAP Rasa3 (GAP1IP4BP) as a major phosphatidylinositol 3,4,5-trisphosphate-binding protein in human platelets and a key regulator of integrin αIIbβ3 outside-in signaling. We demonstrate that cytosolic Rasa3 translocates to the plasma membrane in a PI3K-dependent manner upon activation of human platelets. Expression of wild-type Rasa3 in integrin αIIbβ3-expressing CHO cells blocked Rap1 activity and integrin αIIbβ3-mediated spreading on fibrinogen. In contrast, Rap1GAP-deficient (P489V) and Ras/Rap1GAP-deficient (R371Q) Rasa3 had no effect. We furthermore show that two Rasa3 mutants (H794L and G125V), which are expressed in different mouse models of thrombocytopenia, lack both Ras and Rap1GAP activity and do not affect integrin αIIbβ3-mediated spreading of CHO cells on fibrinogen. Platelets from thrombocytopenic mice expressing GAP-deficient Rasa3 (H794L) show increased spreading on fibrinogen, which in contrast to wild-type platelets is insensitive to PI3K inhibitors. Together, these results support an important role for Rasa3 in PI3K-dependent integrin αIIbβ3-mediated outside-in signaling and cell spreading