ПРИОРИТЕТЫ ОТРАСЛЕВОГО ПРАВОРЕГУЛИРОВАНИЯ

The Book under review: Grechuha V.N. Russian Transport Law: Textbook for MSc students.– Moscow: Uright publishing house, 2012.– 583 pp.The textbook describes the essentials of the origins and specific characteristics of Russian transport law, its institutes and segments, issues of management, freightage, contracts, liability, passenger, luggage and cargo carriage by air, railway, sea and river transport.Гречуха В. Н. Транспортное право  России: Учебник для магистров.– М.:  Юрайт, 2012.– 583 с.Освещены основные положения,  характеризующие природу  и специфику российского  транспортного права, его  подотраслей и институтов,  рассмотрены вопросы управления  в области транспорта, понятия  перевозки, договорных оснований  ее организации и осуществления,  дан анализ ответственности  за нарушение предусмотренных  обязательств. Раскрыты особенности  правового регулирования перевозок  пассажиров, багажа, грузов  автомобильным, железнодорожным,  воздушным, морским и внутренним  водным транспортом

Wigner crystal model of counterion induced bundle formation of rod-like polyelectrolytes

A simple electrostatic theory of condensation of rod-like polyelectrolytes under influence of polyvalent ions is proposed. It is based on the idea that Manning condensation of ions results in formation of the Wigner crystal on a background of a bundle of rods. It is shown that, depending on a single dimensionless parameter, this can be the densely packed three-dimensional Wigner crystal or the two-dimensional crystal on the rod surfaces. For DNA the location of charge on the spiral results in a model of the one-dimensional Wigner crystal. It is also argued that the Wigner crystal idea can be applied to self-assembly of other polyelectrolytes, for example, colloids and DNA-lipid complexes.Comment: 4 pages; typos corrected, references adde

Cell-Derived Microparticles in the Pathogenesis of Cardiovascular Disease Friend or Foe?

Microparticles are ascribed important roles in coagulation, inflammation, and endothelial function. These processes are mandatory to safeguard the integrity of the organism, and their derangements contribute to the development of atherosclerosis and cardiovascular disease. More recently, the presumed solely harmful role of microparticles has been challenged because microparticles may also be involved in the maintenance and preservation of cellular homeostasis and in promoting defense mechanisms. Here, we summarize recent studies revealing these 2 faces of microparticles in cardiovascular disease. (Arterioscler Thromb Vasc Biol. 2011;31:4-9.

АКТУАЛЬНОЕ ИСКУССТВО КАК ОТРАЖЕНИЕ «НОВОЙ ТЕЛЕСНОСТИ»

 This article deals with the transformation of the modern human’s consciousness and the reflection of this process in the contemporary art. The 21st century discovers a «new» corporality, we begin to understand that consciousness of a human hinges on his body. The body begins to acquire a whole new meaning for people, becoming a tool for the development of personal identity. The authors study the contemporary art as a way of comprehending this corporality using the example of the body image of sculptures by Antony Gormley.В статье рассматривается трансформация сознания современного человека и ее отражение в формах актуального искусства. ХХI век открывает «новую» телесность, приходит понимание того, что человеческое сознание напрямую зависит от его тела. Тело начинает обретать иную значимость для человека, становясь инструментом формирования личной идентичности. Авторы исследуют актуальное искусство как способ осмысления этой телесности на примере образа тела в скульптурах Энтони Гормли

THE PROGNOSIS OF DEVELOPMENT OF THE PAROXYSM OF ATRIAL FIBRILLATION IN PATIENTS WITH POST-INFARCTION CARDIOSCLEROSIS

Aim. Create an algorithm predicting the risk of development of paroxysmal atrial fibrillation in patients with coronary heart disease on the background of post-infarction cardiosclerosis (PICS).Material and Methods. There was conducted the deep analysis of a number of laboratory and functional indicators (indicators of peroxidation of proteins, lipids, antioxidant protection and skin microcirculation) at 85 patients with atrial fibrillation (AF). All patients were divided into 4 groups: patients with paroxysmal and chronic AF without PICS (27 people), patients with paroxysmal and chronic AF with PICS (12), patients with permanent AF without PICS (26) and patients with permanent form of AF with PICS (20). Results. There was created the mathematical model to predict the development of atrial fibrillation in patients with PICS with calculation of the odds ratio of atrial fibrillation. Methods of correlation and factor analysis revealed the greatest value for the prognosis of paroxysmal atrial fibrillation in patients with PICS two factors: the total level of deep oxidation products of protein and an indicator of microcirculation by laser Doppler flowmetry. The diagnostic sensitivity of the developed prognostic model was 86%, specificity of the test – 94%, the diagnostic efficiency of the test – 90%.Conclusion. The study has been proven clinical diagnostic and prognostic value of a comprehensive study of indicators of oxidation-reduction reactions, and the microcirculation indices in combination with standard laboratory and instrumental investigations in patients with AF. The developed mathematical model allows to predict the development of atrial fibrillation in patients with PICS

On the use of glutamine-containing specialty foods in sports

Glutamine (GLN) and glutamic acid (GLA) are involved in many metabolic processes, from nucleotide synthesis to nerve impulse conduction; GLN is also an energy substrate for immunocompetent cells, making it an important link in the immune response. In stressful situations, which include significant physical activity, plasma levels of GLN and GLA decrease as a result of the amino acid being actively consumed in many biochemical reactions. GLN deficiency can lead to a number of negative manifestations in athletes and adversely affect athletic performance. The purpose of this work was to study the effects of glutamine deficiency and the possible replenishment of the deficiency by the consumption of exogenous forms of the substance when using glutamine-containing products; special attention was paid to the study of transient decrease in immune function as a relevant factor that impairs the mode of sports training

Production of immunoregulatory molecules by induced erythroblasts at various stages of cell differentiation

Introduction. Bone marrow erythroblasts produce a wide range of cytokines with opposite biological effects. This may be due to a change in the spectrum of production of immunoregulatory mediators during differentiation and small qualitative and quantitative differences in the spectrum of cytokines produced at each stage of differentiation, which may be important for the regulation of hemo- and immunopoiesis.   The aim. To study the spectrum of production of mediators by erythroblasts at different stages of differentiation.   Methods. Erythroblasts were obtained from CD34+ bone marrow cells of healthy donors in the presence of recombinant cytokines. Phenotype assessment was performed using flow cytometry for erythroid (CD45, CD71, CD235a, CD44) and lymphoid markers (CD3, CD4, CD8, CD16, CD19). Blocking of erythroblast differentiation at different stages was carried out using specific blocking monoclonal antibodies to melanocortin receptors (MCR) of types 1, 2 and 5. Cytokine analysis in conditioned erythroblast media was performed using the Bio-Plex Pro Human Cytokine 48-Plex Screening Panel (Bio-Rad Laboratories, USA). Cytokine production was analyzed using the CytokineExplore online tool.   Results. The resulting erythroblasts are divided into positive and negative populations according to the CD45 marker, carry markers of erythroid cells CD71, CD235a and do not express linear markers of lymphoid cells. In type 1 MCR blockage, polychromatophilic erythroblasts predominate, in type 2 MCR blockage, basophilic erythroblasts predominate, and in type 5 MCR blockage, orthochromatophilic erythroblasts accumulate. According to the production of cytokines, it was shown that when using any of the blocking antibodies, we obtain cells that differ qualitatively and quantitatively in a number of mediators from the initial population of induced erythroblasts.   Conclusion. Thus, we have shown qualitative and quantitative differences in the production of mediators by erythroblasts depending on the stage of differentiation, which can lead to different regulatory effects

Prevalence of Risk Factors of Thromboembolic Complications in Women after Major Joint Arthroplasty in the Republic of Sakha (Yakutia)

The aim of this study was to assess the risk factors for thromboembolic complications after total arthroplasty of large joints in women in Yakutia conditions to optimize the management tactics of this category of patients. The average age of women was 59.98±11.56 years in the age range from 50 to 70 years. In order to validate the study, women were divided into 2 groups. The main group consisted of 284 women undergoing total knee arthroplasty (Group 1). The comparison group included 147 women undergoing total hip arthoplasty (Group 2). The study demonstrated that hypertension was more common in patients of Group 1 than in patients of Group 2. However, the incidence of coronary heart disease and heart rhythm disorder was detected most frequently in patients with total hip arthroplasty. Obesity, thrombosis of the veins of the lower extremities, and liver disease were detected with almost the same frequency in women with total knee arthroplasty and those with total hip arthroplasty. The frequency of occurrence of complications depending on the risk factors for thromboembolic complications and the type of surgical treatment of the joint was equal in the two groups of studied patients

Supplementing High-Density SNP Microarrays for Additional Coverage of Disease-Related Genes: Addiction as a Paradigm

Commercial SNP microarrays now provide comprehensive and affordable coverage of the human genome. However, some diseases have biologically relevant genomic regions that may require additional coverage. Addiction, for example, is thought to be influenced by complex interactions among many relevant genes and pathways. We have assembled a list of 486 biologically relevant genes nominated by a panel of experts on addiction. We then added 424 genes that showed evidence of association with addiction phenotypes through mouse QTL mappings and gene co-expression analysis. We demonstrate that there are a substantial number of SNPs in these genes that are not well represented by commercial SNP platforms. We address this problem by introducing a publicly available SNP database for addiction. The database is annotated using numeric prioritization scores indicating the extent of biological relevance. The scores incorporate a number of factors such as SNP/gene functional properties (including synonymy and promoter regions), data from mouse systems genetics and measures of human/mouse evolutionary conservation. We then used HapMap genotyping data to determine if a SNP is tagged by a commercial microarray through linkage disequilibrium. This combination of biological prioritization scores and LD tagging annotation will enable addiction researchers to supplement commercial SNP microarrays to ensure comprehensive coverage of biologically relevant regions

Regulation of PERK Signaling and Leukemic Cell Survival by a Novel Cytosolic Isoform of the UPR Regulator GRP78/BiP

The unfolded protein response (UPR) is an evolutionarily conserved mechanism to allow cells to adapt to stress targeting the endoplasmic reticulum (ER). Induction of ER chaperone GRP78/BiP increases protein folding capacity; as such it represents a major survival arm of UPR. Considering the central importance of the UPR in regulating cell survival and death, evidence is emerging that cells evolve feedback regulatory pathways to modulate the key UPR executors, however, the precise mechanisms remain to be elucidated. Here, we report the fortuitous discovery of GRP78va, a novel isoform of GRP78 generated by alternative splicing (retention of intron 1) and alternative translation initiation. Bioinformatic and biochemical analyses revealed that expression of GRP78va is enhanced by ER stress and is notably elevated in human leukemic cells and leukemia patients. In contrast to the canonical GRP78 which is primarily an ER lumenal protein, GRP78va is devoid of the ER signaling peptide and is cytosolic. Through specific knockdown of endogenous GRP78va by siRNA without affecting canonical GRP78, we showed that GRP78va promotes cell survival under ER stress. We further demonstrated that GRP78va has the ability to regulate PERK signaling and that GRP78va is able to interact with and antagonize PERK inhibitor P58IPK. Our study describes the discovery of GRP78va, a novel cytosolic isoform of GRP78/BiP, and the first characterization of the modulation of UPR signaling via alternative splicing of nuclear pre-mRNA. Our study further reveals a novel survival mechanism in leukemic cells and other cell types where GRP78va is expressed