20 research outputs found
The dental calculus metabolome in modern and historic samples.
INTRODUCTION: Dental calculus is a mineralized microbial dental plaque biofilm that forms throughout life by precipitation of salivary calcium salts. Successive cycles of dental plaque growth and calcification make it an unusually well-preserved, long-term record of host-microbial interaction in the archaeological record. Recent studies have confirmed the survival of authentic ancient DNA and proteins within historic and prehistoric dental calculus, making it a promising substrate for investigating oral microbiome evolution via direct measurement and comparison of modern and ancient specimens. OBJECTIVE: We present the first comprehensive characterization of the human dental calculus metabolome using a multi-platform approach. METHODS: Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) quantified 285 metabolites in modern and historic (200 years old) dental calculus, including metabolites of drug and dietary origin. A subset of historic samples was additionally analyzed by high-resolution gas chromatography-MS (GC-MS) and UPLC-MS/MS for further characterization of metabolites and lipids. Metabolite profiles of modern and historic calculus were compared to identify patterns of persistence and loss. RESULTS: Dipeptides, free amino acids, free nucleotides, and carbohydrates substantially decrease in abundance and ubiquity in archaeological samples, with some exceptions. Lipids generally persist, and saturated and mono-unsaturated medium and long chain fatty acids appear to be well-preserved, while metabolic derivatives related to oxidation and chemical degradation are found at higher levels in archaeological dental calculus than fresh samples. CONCLUSIONS: The results of this study indicate that certain metabolite classes have higher potential for recovery over long time scales and may serve as appropriate targets for oral microbiome evolutionary studies
Dental metric standards for sex estimation in archaeological populations from Iran
Sex estimation of skeletal remains is one of the major components of forensic
identification of unknown individuals. Teeth are a potential source of information on
sex and are often recovered in archaeological or forensic contexts due to their post-mortem
longevity. Currently there is limited data on dental sexual dimorphism of
archaeological populations from Iran. This dissertation represents the first study to
provide a dental sex estimation method for Iron Age populations.
The current study was conducted on the skeletal remains of 143 adults from two Iron
Age populations in close temporal and geographic proximity in the Solduz Valley
(West Azerbaijan Province of Iran). 2D and 3D cervical mesiodistal and buccolingual
and root volume measurements of maxillary and mandibular teeth were used to
investigate the degree of sexual dimorphism in permanent dentition and to assess their
applicability in sex estimation. In total 1327, 457, and 480 anterior and posterior teeth
were used to collect 2D cervical, 3D cervical, and root volume measurements
respectively. 2D cervical measurements were taken using Hillson-Fitzgerald dental
calliper and 3D measurements were collected using CT images provided by Open
Research Scan Archive (ORSA) - Penn Museum. 3D models of the teeth were created
using manual segmentation in the Amira 6.01 software package. Since tooth density
largely differs from crown to apex, root segmentation required two threshold levels:
the segmentation of the root from the jaw and the segmentation of the crown from the
root. Thresholds used for root segmentation were calculated using the half maximum
height protocol of Spoor et al. (1993) for each skull, and thresholds used for crown
segmentation were set visually for each tooth separately. Data was analysed using
discriminant function analysis and posterior probabilities were calculated for all
produced formulae where sex was previously assessed from morphological features of
pelvis and skull. Bootstrapping was used to account for small sample sizes in the
analysis. Statistical analysis was carried out using SPSS 23. The percentage of sexual
dimorphism was also used to quantify the amount of sexual dimorphism in the sample.
The results showed that incisors and canines were the most sexually dimorphic teeth,
providing percentages of correct sex classification between 80% and 100% depending
on the measurement used. Root volume measurement was shown to be the most
sexually dimorphic variable providing an accuracy of over 90% in all functions.
The present study provided the first dental metric standards for sex estimation using
odontometric data in Iranian archaeological populations. Dental measurements,
particularly root volume measurements, were found to be of value for sex assessment
and the method presented here could be a useful tool for establishing accurate
demographic data from skeletal remains of the Iron Age from Iran
Global Geometric Morphometric Analyses of the Human Pelvis Reveal Substantial Neutral Population History Effects, Even across Sexes
Recent applications of population genetic models to human craniodental traits have revealed a strong neutral component to patterns of global variation. However, little work has been undertaken to determine whether neutral processes might also be influencing the postcranium, perhaps due to substantial evidence for selection and plastic environmental responses in these regions. Recent work has provided evidence for neutral effects in the pelvis, but has been limited in regard to shape data (small numbers of linear measurements) and restricted only to males. Here, we use geometric morphometric methods to examine population variation in the human os coxae (pelvic bone) in both males and females. Neutrality is examined via apportionment of variance patterns and fit to an Out-of-Africa serial founder effect model, which is known to structure neutral genetic patterns. Moreover, we compare males and females directly, and the true versus false pelvis, in order to examine potential obstetrical effects. Our results indicate evidence for substantial neutral population history effects on pelvic shape variation. They also reveal evidence for the effect of obstetrical constraints, but these affect males and females to equivalent extents. Our results do not deny an important role for selection in regard to specific aspects of human pelvic variation, especially in terms of features associated with body size and proportions. However, our analyses demonstrate that at a global level, the shape of the os coxae reveals substantial evidence for neutral variation. Our analyses thus indicate that population variation in the human pelvis might be used to address important questions concerning population history, just as the human cranium has done