Genetic variability of anther donor versus spontaneous doubled haploid descendents and colchicine induced doubled haploid sweet pepper (Capsicum annuum L.) lines

Haploid (n) and doubled haploid (DH) plants were developed in anther culture of sweet pepper (Capsicum annuum L.). Regenerants were analyzed by flow cytometry for haploid (n = 12) and spontaneous doubled haploid (2n = 24) genomes. Haploid plants were forwarded to colchicine-treatment for induced doubled haploid (2n·) plant production. Molecular polymorphism of anther donor plants (2n), the haploid regenerants (n), the spontaneous (2n) and induced (2n·)-DH plants were analysed by RAPD-, SSR- and ISSR-PCR. The analysis of anther-donor plants compared to DH-descendents showed an unexpectedly wide range of molecular polymorphism. Our results suggest that genetic changes occurring during meiotic recombination is higher than those of occurring during colchicine-induced genomic duplication

Special Issue Overview: Advances in Remote Sensing and Mapping for Integrated Studies of Reef Ecosystems in Oceania (Great Barrier Reef and Beyond)

The recent widespread and recurrent coral bleaching events over the Great Barrier Reef, the largest coral reef system on Earth and a hotspot of marine biodiversity, are a reminder of the vulnerability of reef ecosystems to human activities and a warming world. Protection of the Great Barrier Reef and similar reef ecosystems across Oceania requires a better understanding of environmental and socio-economic pressures, as well as the development of integrated management strategies. The rapid expansion of Earth Observation technologies and data has greatly advanced our capability to map and monitor reef habitats, ecological processing and exposure risk, providing spatially rich data essential to support and evaluate management and conservation strategies. However, these technologies are proportionally still under-utilized, and it is important to synthesise remote-sensing-derived tools and methods currently available for mapping reef ecosystems in Oceania to facilitate their intake in coral reefs studies. Publications in this Special Issue contribute toward filling this gap and explore recent advances in remote sensing of the Great Barrier Reef and other reef ecosystems in Oceania, from novel methodological approaches (sensors, algorithm development and improved thematic classification) to applications for environmental monitoring and management

Annual report for flood plumes and extreme weather 2013-2014

The Marine Monitoring Program (MMP) undertaken in the Great Barrier Reef lagoon assesses the long-term effectiveness of the Australian and Queensland Government’s Reef Water Quality Protection Plan and the Australian Government Reef Rescue initiative. The MMP was established in 2005 to help assess the long-term status and health of Reef ecosystems and is a critical component in the assessment of regional water quality as land management practices are improved across Reef catchments. The program forms an integral part of the Reef Plan Paddock to Reef Integrated Monitoring, Modelling and Reporting Program supported through Reef Plan and Reef Rescue initiatives. This report details the sampling that has taken place under the Marine Monitoring Program: Annual report for flood plumes and extreme weather 2013-201

Water quality in Princess Charlotte Bay flood plumes and eastern Cape York Peninsula flood plume exposure: 2012-2014

The Marine Monitoring Program (MMP) undertaken in the Great Barrier Reef lagoon assesses the long-term effectiveness of the Australian and Queensland Government’s Reef Water Quality Protection Plan and the Australian Government Reef Rescue initiative. The MMP was established in 2005 to help assess the long-term status and health of Reef ecosystems and is a critical component in the assessment of regional water quality as land management practices are improved across Reef catchments. The program forms an integral part of the Reef Plan Paddock to Reef Integrated Monitoring, Modelling and Reporting Program supported through Reef Plan and Reef Rescue initiatives. This report details the sampling that has taken place under the Marine Monitoring Program in Cape Yor

Using Optical Water-Type Classification in Data-Poor Water Quality Assessment: A Case Study in the Torres Strait

For many years, local communities have expressed concerns that turbid plume waters from the Fly River in Papua New Guinea may potentially deliver mine-derived contaminants to the Torres Strait, an ecologically and culturally unique area north of the Australian mainland. Information on suspended sediment transport and turbidity patterns are needed in this data-limited region to identify and manage downstream ecosystems that may be at risk of exposure from the Fly River runoff. This study used MODIS satellite time series and a colour-classification approach to map optical water types around the data-poor Gulf of Papua and Torres Strait region. The satellite data were supported by field data, including salinity and suspended sediment measurements, and used together in qualitative water quality assessments to evaluate the habitats that are likely exposed to Fly River discharge and/or derived sediments. It showed that the Fly River influence in the Torres Strait region is largely limited to the north-east corner of the Torres Strait. The drivers of turbidity vary between locations, and it is impossible to fully separate direct riverine plume influence from wave and tidally driven sediment resuspension in the satellite maps. However, results indicate that coastal habitats located as far east as Bramble Cay and west to Boigu Island are located in an area that is most likely exposed to the Fly River discharge within the region, directly or through sediment resuspension. The area that is the most likely exposed is a relatively small proportion of the Torres Strait region, but encompasses habitats of high ecological importance, including coral reefs and seagrass meadows. Satellite data showed that the period of highest risk of exposure was during the south-east trade wind season and complemented recent model simulations in the region over larger spatial and temporal frames. This study did not evaluate transboundary pollution or the ecological impact on local marine resources, but other recent studies suggest it is likely to be limited. However, this study did provide long-term, extensive but qualitative, baseline information needed to inform future ecological risk mapping and to support decision making about management priorities in the region. This is important for ensuring the protection of the Torres Strait ecosystems, given their importance to Torres Strait communities and turtle and dugong populations, and the Torres Strait’s connectivity with the Great Barrier Reef Marine Park

Marine Monitoring Program. Final report of JCU activities 2012/13: flood plumes and extreme weather monitoring for the Great Barrier Reef Marine Park Authority

The Reef Rescue Marine Monitoring Program (MMP) undertaken in the Great Barrier Reef lagoon assesses the long-term effectiveness of the Australian and Queensland Government’s Reef Water Quality Protection Plan and the Australian Government Reef Rescue initiative. The MMP was established in 2005 to help assess the long-term status and health of Reef ecosystems and is a critical component in the assessment of regional water quality as land management practices are improved across Reef catchments. The program forms an integral part of the Reef Plan Paddock to Reef Integrated Monitoring, Modelling and Reporting Program supported through Reef Plan and Reef Rescue initiatives. This report details the sampling that has taken place under the Reef Rescue Marine Monitoring Program: Terrestrial discharge into the Great Barrier Reef for the 2012/13 sampling year.Report No. 15/5

Can Forel–Ule Index Act as a Proxy of Water Quality in Temperate Waters? Application of Plume Mapping in Liverpool Bay, UK

The use of ocean colour classification algorithms, linked to water quality gradients, can be a useful tool for mapping river plumes in both tropical and temperate systems. This approach has been applied in operational water quality programs in the Great Barrier Reef to map river plumes and assess trends in marine water composition and ecosystem health during flood periods. In this study, we used the Forel–Ule colour classification algorithm for Sentinel-3 OLCI imagery in an automated process to map monthly, annual and long-term plume movement in the temperate coastal system of Liverpool Bay (UK). We compared monthly river plume extent to the river flow and in situ water quality data between 2017–2020. The results showed a strong positive correlation (Spearman’s rho = 0.68) between the river plume extent and the river flow and a strong link between the FUI defined waterbodies and nutrients, SPM, turbidity and salinity, hence the potential of the Forel–Ule index to act as a proxy for water quality in the temperate Liverpool Bay water. The paper discusses how the Forel–Ule index could be used in operational water quality programs to better understand river plumes and the land-based inputs to the coastal zones in UK waters, drawing parallels with methods that have been developed in the GBR and Citclops project. Overall, this paper provides the first insight into the systematic long-term river plume mapping in UK coastal waters using a fast, cost-effective, and reproducible workflow. The study created a novel water assessment typology based on the common physical, chemical and biological ocean colour properties captured in the Forel–Ule index, which could replace the more traditional eutrophication assessment regions centred around strict geographic and political boundaries. Additionally, the Forel–Ule assessment typology is particularly important since it identifies areas of the greatest impact from the land-based loads into the marine environment, and thus potential risks to vulnerable ecosystems

Impact of a Tropical Cyclone on Terrestrial Inputs and Bio-Optical Properties in Princess Charlotte Bay (Great Barrier Reef Lagoon)

In January 2013, tropical cyclone Oswald caused widespread flooding in the North-East coast of Australia, and large and highly episodic inputs into Princess Charlotte Bay (PCB, northern Great Barrier Reef). Freshwater outflows from the Normanby and Kennedy rivers, the two main rivers draining the adjacent catchments, resulted in drastic changes in physical, biogeochemical and optical properties within PCB. On 31 January, 2 days after the peak riverine discharge from the Normanby river, nutrients and dissolved organic matter contents peaked under the influence of large outflows from the Kennedy river into the western section of the bay (5.8 μM for dissolved inorganic nitrogen, 6.9 g m−3 for dissolved organic carbon and 6.1 m−1 for the colored dissolved organic matter absorption coefficient at 412 nm). In the eastern section of the bay, the situation appeared more ‘mixed’, with a suspended solids concentration reaching 23.1 g m−3 close to the Normanby river mouth. The main phytoplankton bloom occurred in the transition zone between the Kennedy and Normanby flood plumes, and was dominated by diatoms with a chlorophyll a concentration reaching 14.6 mg m−3. This study highlights the need to better describe the critical spatial and temporal scales of variability of key biogeochemical and optical properties after a major flood event. The data collected is key to improve the accuracy of ocean color remote sensing algorithms and regional biogeochemical budgets following highly episodic inputs

Baseline assessment of coastal water quality, in Vanuatu, South Pacific: insights gained from in-situ sampling

Nearshore deterioration of water quality in Pacific coastal waters is a growing problem, associated with increasing urban and industrial sewage discharges, and agricultural runoff. Published water quality studies in the Pacific region are limited in both number and scope, making it difficult to resolve the extent of the issue or quantify the variability of water quality across Pacific islands and countries. This study collected water quality measurements over three years in the coastal waters around the Island of Efate (Vanuatu) with majority of work carried out in Port Vila, its capital. Port Vila is the key urban centre for Vanuatu where the increasing population and pollution inputs are placing substantial pressure on the coastal environment. Highest concentrations of dissolved nutrients and suspended sediments were measured adjacent or near the urban drains that enter the coastal areas along the capital's seafront, highlighting many of the issues around anthropogenic inputs are linked to the increasing urbanisation in Port Vila Bay. We provide baseline data that explores variability of coastal water quality and these types of datasets for Pacific islands are a first step towards facilitating development of long-term monitoring programmes and informing coastal zone management decision making

2017 Scientific Consensus Statement: land use impacts on the Great Barrier Reef water quality and ecosystem condition, Chapter 3: the risk from anthropogenic pollutants to Great Barrier Reef coastal and marine ecosystems

In this chapter, we applied an ecological risk assessment approach to assess the likelihood of exposure and potential risks from land-based pollutants to Great Barrier Reef coastal (floodplain wetlands and floodplains) and marine (coral reefs and seagrass meadows) ecosystems. Ecological risk is defined as the product of the likelihood of an effect occurring and the consequences if that effect was to occur