Interactional co-design and co-production through shared dialogue workshops

In 2017 660 million people remain without sustainable access to safe drinking water [1]. The majority of these are in rural areas with little hope in the foreseeable future of access to distributed treated water systems. Solar water disinfection (SODIS) is a household water treatment using solar energy to inactivate pathogens in water stored in transparent containers placed in direct sunlight. SODIS is used by approximately 5 million people in developing countries daily [2], but uptake is slowing. The WATERSPOUTT project aims to increase user uptake of SODIS by designing, piloting and manufacturing technologies including solar jerry cans and solar-ceramic filtration. These are being designed in a multi-disciplinary collaboration between designers, engineers, health and social scientists and end users in Europe and Africa. This is achieved through co-design activities, context analysis and stakeholder dialogue workshops which aim to ensure that product designs meet both the technical and social needs of the more than 100 million potential end users in Africa. Examples of student design work highlight the importance of this shared dialogue and changes in design thinking that are evolving through the co-design approach. Through producing designs which are readily accepted and widely adopted in the case study communities, this paper addresses issues relevant to the topics of social issues in design education and new design education paradigms. It also addresses the wider theme of building community: design education for a sustainable future by showing how transdisciplinary approaches can ensure community engagement and design adoption

Population Genetic Structuring in Opisthorchis viverrini over Various Spatial Scales in Thailand and Lao PDR

Khon Kaen Province in northeast Thailand is known as a hot spot for opisthorchiasis in Southeast Asia. Preliminary allozyme and mitochondrial DNA haplotype data from within one endemic district in this Province (Ban Phai), indicated substantial genetic variability within Opisthorchis viverrini. Here, we used microsatellite DNA analyses to examine the genetic diversity and population structure of O. viverrini from four geographically close localities in Khon Kaen Province. Genotyping based on 12 microsatellite loci yielded a mean number of alleles per locus that ranged from 2.83 to 3.7 with an expected heterozygosity in Hardy-Weinberg equilibrium of 0.44-0.56. Assessment of population structure by pairwise F(ST) analysis showed inter-population differentiation (P<0.05) which indicates population substructuring between these localities. Unique alleles were found in three of four localities with the highest number observed per locality being three. Our results highlight the existence of genetic diversity and population substructuring in O. viverrini over a small spatial scale which is similar to that found at a larger scale. This provides the basis for the investigation of the role of parasite genetic diversity and differentiation in transmission dynamics and control of O. viverrini

The use of domestic chickens as laboratory hosts of the larvae of the bont tick, Amblyomma hebraeum

A comparison of attachment, engorgement and moulting success of Amblyomma hebraeum larvae fed on domestic chickens and Himalayan giant white rabbits indicates that chickens are better hosts than the rabbits. Moreover the time needed for detachment of all engorged larvae is significantly less for chickens than for the rabbits. No evidence of induced immunity was found in chickens on re-infestation with larvae of A. hebraeum.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

Evolutionary divergence of the swim bladder nematode Anguillicola crassus after colonization of a novel host, Anguilla anguilla

BACKGROUND: Anguillicola crassus, a swim bladder nematode naturally parasitizing the Japanese eel, was introduced about 30 years ago from East Asia into Europe where it colonized almost all populations of the European eel. We conducted a common garden experiment under a reciprocal transfer design infecting both European and Japanese eels with populations of A. crassus from Germany, Poland and Taiwan. We tested, whether differences in infectivity, developmental dynamics and reproductive output between the European and Asian parasite populations occur while harboured in the specimens of native and colonized eel host, and if these differences are genetically based or are plastic responses to the new environment. RESULTS: Under common garden conditions an evolutionary change in the both European parasite populations of A. crassus compared with their Taiwanese conspecifics was observed for infectivity and developmental dynamics, but not for reproductive output. When infecting the European eel, current European populations of the parasite were less infective and developed faster than their Taiwanese conspecifics. In the reciprocally infected Japanese eel the genetically induced differences between the parasite strains were less apparent than in the European eel but higher infectivity, faster development and higher larval mortality of the European parasite populations could be inferred. CONCLUSIONS: The differences in infectivity and developmental dynamics between European and Taiwanese populations of A. crassus found in our study suggest rapid genetic divergence of this parasite after a successful host switch in Europe

Genetic diversity of the red-spotted tokay gecko (Gekko gecko Linnaeus, 1758) (Squamata: Gekkonidae) in Southeast Asia determined with multilocus enzyme electrophoresis

Red-spotted tokay geckos, Gekko gecko, are distributed mainly in Southeast Asia. They are a traditional Chinese medicine, with the massive hunting for exports dramatically decreasing their numbers. Information on the genetic diversity of these geckos in Southeast Asia is very limited. This study aims to explore intrapopulation and interpopulation genetic variation and the genetic structure of 16 populations collected from different localities in Thailand, Lao People’s Democratic Republic, and Cambodia using multilocus enzyme electrophoresis. Relatively high genetic diversity occurred at both the intrapopulation and interpopulation levels. Genetic differentiation with F$_{ST}$ values ranging between 0.006 - 0.892 was found. Five distinct genetic groups of the red-spotted tokay populations could be classified. A group of populations from northern Thailand showed the highest genetic differentiation from the other groups. Moreover, there was a substantial genetic subdivision depending on the genetic groups with F$_{CT}$ = 0.664 and F$_{SC}$ = 0.185. This genetic structure is related to geographical distribution and distance between populations, R$^{2}$ = 0.5614, p < 0.001. Our findings of pronounced genetic structuring and the concomitant conservation genetic consequences if further population loss occurs mean that management actions should therefore focus on the conservation of all of the main sites where tokay geckos still occur

On sampling tick populations : the problem of overdispersion

Data collected on both free-living and parasitic tick populations are likely to be overdispersed. The use of means from few replicate samples of overdispersed data as quantitative estimators of tick population density is in turn likely to lead to inaccurate interpretations which may be scientifically misleading. In this paper ways of estimating overdispersion are listed and suggestions for the use of correct statistical tests for handling overdispersed data are given.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.mn201

The host status of the striped mouse, Rhabdomys pumilio, in relation to the tick vectors of heartwater in South Africa

Striped mice, Rhabdomys pumilio, were trapped over a 17 month period in the Thomas Baines Nature Reserve in the eastern Cape Province of South Africa. Captured mice were placed in cages, over water, and naturally detaching ticks were collected. Only 5 larvae and 1 nymph of Amblyomma hebraeum were recovered from the mice. These represented less than 0,1 % of the total number of ticks recovered. No Amblyomma marmoreum were recovered. As the vegetation and large mammals and tortoises in the Thomas Baines Nature Reserve abound in larvae of both these vectors of heartwater the low infestation rates of R. pumilio indicate that it is either unsuitable or inaccessible for these ticks. R. pumilio is therefore unlikely to play a role in the epidemiology of heartwater.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201

The developmental success of Amblyomma hebraeum and Amblyomma marmoreum on the leopard tortoise, Geochelone pardalis.

The success of natural infestations of various life history stages of Amblyomma hebraeum and Amblyomma marmoreum on the leopard tortoise, Geochelone pardalis, was compared. Success was measured by the time taken for ticks to detach, as well as the percentage of ticks engorging and subsequently either moulting to the next life history stage or laying viable eggs. Larvae of A. hebraeum were the only developmental stage not recovered. Nymphae and female A. hebraeum were less successful in moulting or laying eggs than the corresponding stages of A. marmoreum. Nevertheless, 48,7 % of A. hebraeum nymphae moulted, while 1 of 6 females laid viable eggs.The articles have been scanned in colour with a HP Scanjet 5590; 600dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format.lmchunu2014mn201