239 research outputs found
Assignment of <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N resonances to the sensory domain of the membraneous two-component fumarate sensor (histidine protein kinase) DcuS of Escherichia coli.
Presentation of the HUSEEDwild – a seed weight and germination database of the Pannonian flora – through analysing life forms and social behaviour types
Sinemurian–Pliensbachian calcareous nannofossil biostratigraphy and organic carbon isotope stratigraphy in the Paris Basin: Calibration to the ammonite biozonation of NW Europe
This is the author accepted manuscript. The final version is available from the publisher via the DOI in this record.Available online 12 December 2016The biostratigraphy of Sinemurian to lower Toarcian calcareous nannofossils has been investigated in the Sancerre-Couy core (Paris Basin), which contains a mixed assemblage of species with affinities to the northern and southern areas of the peri-tethyan realm, thus allowing for the use and calibration of the Mediterranean Province (Italy/S France) and NW Europe (UK) biozonation schemes. This study is based on semi-quantitative analyses of the calcareous nannofossil assemblage performed on 145 samples and the recorded bioevents are calibrated to the NW European Ammonite Zonation and to a new organic carbon isotope curve based on 385 data points. The main bioevents, i.e. the first occurrences of Parhabdolithus liasicus, Crepidolithus pliensbachensis, Crepidolithus crassus, Mitrolithus lenticularis, Similiscutum cruciulus sensu lato, Lotharingius hauffii, Crepidolithus cavus and Lotharingius sigillatus as well as the last occurrence of Parhabdolithus robustus, have been identified. However, we show that a large number of standard biostratigraphic markers show inconsistent occurrences at the base and top of their range, possibly accounting for some of the significant discrepancies observed between the different domains. In addition to the nine main bioevents used for the biozonation of the core, we document an additional 50 distinct bioevents, evaluate their reliability and discuss their potential significance by comparison to previous studies. A total of five significant negative organic carbon isotope excursions are identified and defined in the Paris Basin including the well-documented Sinemurian–Pliensbachian boundary event. One positive excursion is further defined in the Pliensbachian interval. Our calibration of high-resolution calcareous nannofossil biostratigraphy to ammonite biostratigraphy and organic carbon isotopes represents a new stratigraphic reference for the Lower Jurassic series
The Ki-67 and Repoman mitotic phosphatases assemble via an identical, yet novel mechanism
Ki-67 and RepoMan have key roles during mitotic exit. Previously (Booth et al, eLife, 2014), we showed that Ki-67 organizes the mitotic chromosome periphery and recruits protein phosphatase 1 (PP1) to chromatin at anaphase onset, in a similar manner as RepoMan. Here we show how Ki-67 and RepoMan form mitotic exit phosphatases by recruiting PP1, how they distinguish between distinct PP1 isoforms and how the assembly of these two holoenzymes are dynamically regulated by Aurora B kinase during mitosis. Unexpectedly, our data also reveal that Ki-67 and RepoMan bind PP1 using an identical, yet entirely novel mechanism, interacting with a PP1 pocket that is engaged only by these two PP1 regulators. These findings not only show how two distinct mitotic exit phosphatases are recruited to their substrates, but also provides immediate opportunities for the design of novel cancer therapeutics that selectively target the Ki-67:PP1 and RepoMan:PP1 holoenzymes
Seed collection data encompassing half of the vascular flora of the pannonian ecoregion stored by the pannon seed bank
Seed bank collections have multiple benefits: store genetic material for conservation and research, and their data can also provide valuable scientific information. The Pannon Seed Bank was established during an EU LIFE+ project between 2010 and 2014 with the target to collect and store seeds of approx. 50% of the wild native vascular flora of the Pannonian Biogeographic Region, seed accessions of at least 800 storable species. This task was fully achieved by the end of the project, as altogether 1,853 seed accessions of 910 species are stored. The aim of the present paper is to provide access to the collection data and metadata of the Pannon Seed Bank as it was completed by the end of the project. The collection campaign involved about 40 experts and covered the whole country. Collection and storing applied standard methodology, based on the ENSCONET project. The collection data published in this paper can be used manifold. Geographical data on species occurrences are major input for nature conservation and research. Seed collection date is valuable for ecological studies of phytophagous insects, frugivorous birds and mammals, etc. The database can be partner to international databases (like GBIF) or research infrastructures (e.g. LifeWatch). Hopefully, this data paper will contribute to further motivate the development of native seed collections and their use for conservation and research in Hungary. © 2016 Akadémiai Kiadó, Budapest
New measurements of thousand-seed weights of species in the Pannonian flora
For understanding local and regional seed dispersal and plant establishment processes and for considering the ecotypes and other forms of specific variability, hard data of locally or regionally measured traits are necessary. We provided newly measured seed weight data of 193 taxa, out of which 24 taxa had not been represented in the SID, LEDA or BiolFlor databases. Our new measurements and formerly published data of locally collected seed weight records together covers over 70% of the Pannonian flora. However, there is still a considerable lack in seed weight data of taxonomically problematic genera, even though they are represented in the Pannonian flora with a relatively high number of species and/or subspecies (e.g. Sorbus, Rosa, Rubus, Crataegus and Hieracium). Our regional database contains very sporadic data on aquatic plants (including also numerous invasive species reported from Hungary and neighbouring countries) and some rare weeds distributed in the southwestern part of the country. These facts indicate the necessity of further seed collection and measurements
The novel Fh8 and H fusion partners for soluble protein expression in Escherichia coli : a comparison with the traditional gene fusion technology
The Escherichia coli host system is an advantageous choice for simple and inexpensive recombinant protein production but it still presents bottlenecks at expressing soluble proteins from other organisms. Several efforts have been taken to overcome E. coli limitations, including the use of fusion partners that improve protein expression and solubility. New fusion technologies are emerging to complement the traditional solutions. This work evaluates two novel fusion partners, the Fh8 tag (8 kDa) and the H tag (1 kDa), as solubility enhancing tags in E. coli and their comparison to commonly used fusion partners. A broad range comparison was conducted in a small-scale screening and subsequently scaled-up. Six difficult-to-express target proteins (RVS167, SPO14, YPK1, YPK2, Frutalin and CP12) were fused to eight fusion tags (His, Trx, GST, MBP, NusA, SUMO, H and Fh8). The resulting protein expression and solubility levels were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis before and after protein purification and after tag removal. The Fh8 partner improved protein expression and solubility as the well-known Trx, NusA or MBP fusion partners. The H partner did not function as a solubility tag. Cleaved proteins from Fh8 fusions were soluble and obtained in similar or higher amounts than proteins from the cleavage of other partners as Trx, NusA or MBP. The Fh8 fusion tag therefore acts as an effective solubility enhancer, and its low molecular weight potentially gives it an advantage over larger solubility tags by offering a more reliable assessment of the target protein solubility when expressed as a fusion protein.The financial support of the EMBL Heidelberg, Germany and Fundacao para a Ciencia e Tecnologia (FCT), Portugal, is acknowledged: the fellowship SFRH/BD/46482/2008 to Sofia J. Costa and the project PTDC/CVT/103081/2008. The authors wish to acknowledge Anne-Claude Gavin for providing four of the constructs for this study (RVS167, SPO14, YPK1, and YPK2) and Emmanuel Poilpre for the experimental help (both from the EMBL Heidelberg, Germany)
Producing the nation through philanthropy: legitimizing co-ethnic and pro-refugee civic action in Hungary
Three Dimensional Structure of the MqsR:MqsA Complex: A Novel TA Pair Comprised of a Toxin Homologous to RelE and an Antitoxin with Unique Properties
One mechanism by which bacteria survive environmental stress is through the formation of bacterial persisters, a sub-population of genetically identical quiescent cells that exhibit multidrug tolerance and are highly enriched in bacterial toxins. Recently, the Escherichia coli gene mqsR (b3022) was identified as the gene most highly upregulated in persisters. Here, we report multiple individual and complex three-dimensional structures of MqsR and its antitoxin MqsA (B3021), which reveal that MqsR:MqsA form a novel toxin:antitoxin (TA) pair. MqsR adopts an α/β fold that is homologous with the RelE/YoeB family of bacterial ribonuclease toxins. MqsA is an elongated dimer that neutralizes MqsR toxicity. As expected for a TA pair, MqsA binds its own promoter. Unexpectedly, it also binds the promoters of genes important for E. coli physiology (e.g., mcbR, spy). Unlike canonical antitoxins, MqsA is also structured throughout its entire sequence, binds zinc and coordinates DNA via its C- and not N-terminal domain. These studies reveal that TA systems, especially the antitoxins, are significantly more diverse than previously recognized and provide new insights into the role of toxins in maintaining the persister state
Molecular mechanism of edema formation in nephrotic syndrome: therapeutic implications
Sodium retention and edema are common features of nephrotic syndrome that are classically attributed to hypovolemia and activation of the renin–angiotensin–aldosterone system. However, numbers of clinical and experimental findings argue against this underfill theory. In this review we analyze data from the literature in both nephrotic patients and experimental models of nephrotic syndrome that converge to demonstrate that sodium retention is not related to the renin–angiotensin–aldosterone status and that fluid leakage from capillary to the interstitium does not result from an imbalance of Starling forces, but from changes of the intrinsic properties of the capillary endothelial filtration barrier. We also discuss how most recent findings on the cellular and molecular mechanisms of sodium retention has allowed the development of an efficient treatment of edema in nephrotic patients
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