Spatial and seasonal relationships between Pacific harbor seals (Phoca vitulina richardii) and their prey, at multiple scales

Knowing where pinnipeds forage is vital to managing and protecting their populations, and for assessing potential interactions with fisheries. We assessed the spatial relationship between the seasonal distribution of Pacific harbor seals (Phoca vitulina richardii) outfitted with satellite transmitters and the seasonal distributions of potential harbor seal prey species in San Francisco Bay, California. Pearson’s correlation coefficients were calculated between the number of harbor seal locations in an area of the San Francisco Bay and the abundance of specific prey species in the same area. The influence of scale on the analyses was assessed by varying the scale of analysis from 1 to 10 km. There was consistency in the prey species targeted by harbor seals year-round, although there were seasonal differences between the most important prey species. The highest correlations between harbor seals and their prey were found for seasonally abundant benthic species, located within about 10 km of the primary haul-out site. Probable foraging habitat for harbor seals was identified, based on areas with high abundances of prey species that were strongly correlated with harbor seal distribution. With comparable local data inputs, this approach has potential application to pinniped management in other areas, and to decisions about the location of marine reserves designed to protect these species

Heat tolerance of short-beaked echidnas (Tachyglossus aculeatus) in the field

(1) Echidnas occur throughout the hot arid zone of Australia yet laboratory studies have concluded that they are ill equipped physiologically to manage T higher than 35°C. (2) Consequently, it is generally assumed that echidnas must rely on behavioural thermoregulation, being nocturnal in hot weather and seeking less extreme microclimates during the day. (3) By monitoring T of echidnas in the field and relating these to T within their day time shelters in Western Queensland during summer, this study showed that echidnas are able to tolerate T of 35-40°C in hollow logs for up to 10 h. (4) Further, as T remains < T in these situations, echidnas may have physiological mechanisms for dealing with the heat after all

A quality of service framework for adaptive and dependable large scale system-of-systems

There is growing recognition within industry that for system growth to be sustainable, the way in which existing assets are used must be improved. Future systems are being developed with a desire for dynamic behaviour and a requirement for dependability at mission critical and safety critical levels. These levels of criticality require predictable performance and as such have traditionally not been associated with adaptive systems. The software architecture proposed for such systems is based around a publish/subscribe model, an approach that, while adaptive, does not typically support critical levels of performance. There is, however, the scope for dependability within such architectures through the use of Quality of Service (QoS) methods. QoS is used in systems where the distribution of resources cannot be decided at design time. A QoS based framework is proposed for providing adaptive and dependable behaviour for future large-scale system-of-systems. Initial simulation results are presented to demonstrate the benefits of QoS

A quality of service framework for dependability in large-scale distributed systems

As recognition grows within industry for the advantages that can be gained through the exploitation of large-scale dynamic systems, a need emerges for dependable performance. Future systems are being developed with a requirement to support mission critical and safety critical applications. These levels of criticality require predictable performance and as such have traditionally not been associated with adaptive systems. The software architecture proposed for such systems takes its properties from the service-oriented computing paradigm and the communication model follows a publish/subscribe approach. While adaptive, such architectures do not, however, typically support real-time levels of performance. There is scope, however, for dependability within such architectures through the use of Quality of Service (QoS) methods. QoS is used in systems where the distribution of resources cannot be decided at design time. In this paper a QoS based framework is proposed for providing adaptive and dependable behaviour for future large-scale dynamic systems through the flexible allocation of resources. Simulation results are presented to demonstrate the benefits of the QoS framework and the tradeoffs that occur between negotiation algorithms of varying complexities

Adaptive architectures for future highly dependable, real time systems

Many present-day safety-critical or mission-critical military applications are deployed using intrinsically static architectures. Often these applications are real-time systems, where late responses may cause potentially catastrophic results. Static architectures allow system developers to certify with a high degree of confidence that their systems will provide correct functionality during operation, but a more adaptive approach could provide some clear benefits. In particular, the ability to dynamically reconfigure the system at run time would give increased flexibility and performance in response to unpredictable or unplanned operating scenarios. Many current dynamic architectural approaches provide little or no features to facilitate the highly dependable, real-time performance required by critical systems. The challenge is to provide the features and benefits of dynamic architectural approaches while still achieving the required level of performance and dependability. This paper describes the early results of an ongoing research programme, part funded by the Software Systems Engineering Initiative (SSEI), aimed at developing a more adaptive software architecture for future military systems. A range of architectures with adaptive features (including object-based, agent based and publish/subscribe) are reviewed against the desirable characteristics of highly dependable systems. A publish/subscribe architecture is proposed as a potential way forward and a discussion of its advantages and disadvantages for highly dependable, real-time systems is given

Gene expression in Leishmania is regulated predominantly by gene dosage

ABSTRACT Leishmania tropica, a unicellular eukaryotic parasite present in North and East Africa, the Middle East, and the Indian subcontinent, has been linked to large outbreaks of cutaneous leishmaniasis in displaced populations in Iraq, Jordan, and Syria. Here, we report the genome sequence of this pathogen and 7,863 identified protein-coding genes, and we show that the majority of clinical isolates possess high levels of allelic diversity, genetic admixture, heterozygosity, and extensive aneuploidy. By utilizing paired genome-wide high-throughput DNA sequencing (DNA-seq) with RNA-seq, we found that gene dosage, at the level of individual genes or chromosomal “somy” (a general term covering disomy, trisomy, tetrasomy, etc.), accounted for greater than 85% of total gene expression variation in genes with a 2-fold or greater change in expression. High gene copy number variation (CNV) among membrane-bound transporters, a class of proteins previously implicated in drug resistance, was found for the most highly differentially expressed genes. Our results suggest that gene dosage is an adaptive trait that confers phenotypic plasticity among natural Leishmania populations by rapid down- or upregulation of transporter proteins to limit the effects of environmental stresses, such as drug selection. IMPORTANCE Leishmania is a genus of unicellular eukaryotic parasites that is responsible for a spectrum of human diseases that range from cutaneous leishmaniasis (CL) and mucocutaneous leishmaniasis (MCL) to life-threatening visceral leishmaniasis (VL). Developmental and strain-specific gene expression is largely thought to be due to mRNA message stability or posttranscriptional regulatory networks for this species, whose genome is organized into polycistronic gene clusters in the absence of promoter-mediated regulation of transcription initiation of nuclear genes. Genetic hybridization has been demonstrated to yield dramatic structural genomic variation, but whether such changes in gene dosage impact gene expression has not been formally investigated. Here we show that the predominant mechanism determining transcript abundance differences (>85%) in Leishmania tropica is that of gene dosage at the level of individual genes or chromosomal somy

Mating and Male Combat in Australian Blacksnakes, Pseudechis porphyriacus

We observed ritual combat and other agonistic behavior between male blacksnakes (Pseudechis porphyriacus) in a field population in central New South Wales. Combat bouts vary greatly in duration (2 to 30 minutes), and apparently function as male "strategies" to displace rival courting males. Direct attack and biting may serve to displace rival males in copulo. We briefly review published literature on male combat in snakes

Acute exposure of mice to high-dose ultrafine carbon black decreases susceptibility to pneumococcal pneumonia

<p>Abstract</p> <p>Background</p> <p>Epidemiological studies suggest that inhalation of carbonaceous particulate matter from biomass combustion increases susceptibility to bacterial pneumonia. <it>In vitro </it>studies report that phagocytosis of carbon black by alveolar macrophages (AM) impairs killing of <it>Streptococcus pneumoniae</it>. We have previously reported high levels of black carbon in AM from biomass smoke-exposed children and adults. We therefore aimed to use a mouse model to test the hypothesis that high levels of carbon loading of AM <it>in vivo </it>increases susceptibility to pneumococcal pneumonia.</p> <p>Methods</p> <p>Female outbred mice were treated with either intranasal phosphate buffered saline (PBS) or ultrafine carbon black (UF-CB in PBS; 500 μg on day 1 and day 4), and then infected with <it>S. pneumoniae </it>strain D39 on day 5. Survival was assessed over 72 h. The effect of UF-CB on AM carbon loading, airway inflammation, and a urinary marker of pulmonary oxidative stress was assessed in uninfected animals.</p> <p>Results</p> <p>Instillation of UF-CB in mice resulted a pattern of AM carbon loading similar to that of biomass-smoke exposed humans. In uninfected animals, UF-CB treated animals had increased urinary 8-oxodG (P = 0.055), and an increased airway neutrophil differential count (P < 0.01). All PBS-treated mice died within 72 h after infection with S<it>. pneumoniae</it>, whereas morbidity and mortality after infection was reduced in UF-CB treated animals (median survival 48 h vs. 30 h, P < 0.001). At 24 hr post-infection, UF-CB treated mice had lower lung and the blood S<it>. pneumoniae </it>colony forming unit counts, and lower airway levels of keratinocyte-derived chemokine/growth-related oncogene (KC/GRO), and interferon gamma.</p> <p>Conclusion</p> <p>Acute high level loading of AM with ultrafine carbon black particles <it>per se </it>does not increase the susceptibility of mice to pneumococcal infection <it>in vivo</it>.</p

Relations among math self-efficacy, interest, intentions, and achievement: a social cognitive perspective

Drawing on social cognitive perspectives, the present study examined an integrative model of the interplay among math self-efficacy, interests, aspirations, and achievement among early and middle adolescents. Based on short-term longitudinal data from approximately 400 students, analyses using fully latent structural equation analyses, establishing requisite levels of longitudinal invariance, revealed that (a) math self-efficacy positively predicted math achievement using both class grades and standardized test score operationalizations; (b) prior math achievement positively predicted basal levels of math self-efficacy but not changes in self-efficacy; (c) math interest and intentions were reciprocally linked over time; and (d) prior math interest positively predicted subsequent math self-efficacy whereas the opposite was not true. Notably, all effects were observed while accounting for prior variance in outcomes as well as the effects of known covariates. The current findings contribute to understandings of the motivational processes involved in math achievement and choosing educational pathways, and suggest that multidimensional interventions may be most profitable if both achievement and selection outcomes are at stake

THE RESPONSE OF MECHANORECEPTORS IN RAT SKIN TO BIAXIAL LOADING: A PRELIMINARY STUDY

ABSTRACT Mechanoreceptor neurons were studied in an isolated rat skin preparation subjected to dynamic biaxial stretch. The strength of the relationship between neuronal responses and mechanical variables was determined using multiple logistic regression. The experimental protocol allowed the normal stresses as well as the maximum shear stress to be manipulated. In n=4 neurons, response was associated with the time rate of change of normal stress and this response was affected by the direction of loading. There was no relationship between response and the value of maximum shear stress. There was a strong association with the rate of change of maximum shear stress. INTRODUCTION Mechanoreceptors are sensory neurons that are activated by mechanical stimulation of soft tissues. There are large numbers of mechanoreceptors in skin, and virtually all of them can be activated by stretch stimuli. Stretching skin alters the states of both stress and strain. Work in our laboratory has focused on determining the coupling between stretch-evoked responses in cutaneous neurons and the stress and strain components of stretch stimuli. Recently