99 research outputs found
Population Structure of Denison’s barb, Puntius denisonii (Pisces: Cyprinidae): A Species Complex Endemic to the Western Ghats of India
Genetic and morphologic variation, haplotype relationships, and structuring of populations within Puntius denisonii
and its close related species Puntius chalakkudiensis have been tested using molecular and biometric data, to infer
phylogeographic patterns. Sequences of mitochondrial DNA ATPase 8 and 6 genes, and morphometric data, were
used to find population structuring. Specimens were collected from 7 locations in the southern region of Western
Ghats, a global biodiversity hotspot in India. Biometric analysis revealed apparent heterogeneity in the morphology
and color pattern between the species at juvenile and adult stages, and among different geographically separated
populations of these species. High values for mean pair wise distances and a high proportion of the total variance
attributed to differences between the geographically isolated populations with AMOVA, indicated clear population
structuring within these species. Extremely high values for Pair wise FST and significantly lower Nm values observed
among the populations studied, suggested little or no effective gene flow among them. Constructed phylogenies
further confirmed a high degree of population structuring within the species, showing local endemism with population
specific haplotypes forming a species complex. The present study thus estimates the validity of subpopulations
within P. denisonii and P. chalakkudiensis; clarifies the relationships of populations of P. denisonii with that of P.
chalakkudiensis, and also indicates the presence of four different independent evolutionary lineages forming cryptic
species within P. denisonii. The study further emphasizes the need for a conservation policy to be developed for each
population of both species, separately based on MUs (Management Units)
Clinical applications of Fast Field‐Cycling techniques
Peer reviewedPublisher PD
Techniques and Applications of Field-Cycling MRI
Non peer reviewedPublisher PD
Techniques and Bio-Medical Applications of Field-Cycling Magnetic Resonance
Non peer reviewedPublisher PD
Training Manual In the frame work of the project: DBT sponsored Three Months National Training in Molecular Biology and Biotechnology for Fisheries Professionals 2015-18
This is a limited edition of the CMFRI Training Manual provided to
participants of the “DBT sponsored Three Months National Training
in Molecular Biology and Biotechnology for Fisheries Professionals”
organized by the Marine Biotechnology Division of Central Marine
Fisheries Research Institute (CMFRI), from 2nd February 2015 - 31st
March 2018
Species of a whale and an unknown fish sample identified using molecular taxonomy
Molecular genetics provides a powerful tool for conservation of species protected by international
regulations or threatened by overexploitation. The present communication is the first report from
India on the application of molecular tools for the accurate identification of a stranded whale in
putrefied condition as it was impossible to identify the species status using conventional taxonomy
and the carcass of an unknown animal devoid of its head and tail, collected from a fish market.
Partial sequences of mtDNA control region and cytochrome b gene of the whale were generated and
tested with BLAST search and DNA surveillance for molecular identification. It was identified as
Bryde's whale (Balaenoptera edeni). Partial sequence of mtDNA cytochrome b gene of the unknown
fish from the market was generated, tested with BLAST search and was identified as sword fish
Xiphias gladius
Field-Cycling Magnetic Resonance Imaging - a Curiosity or the Next Big Thing?
Non peer reviewedPublisher PD
Identification of microsatellite loci, gene ontology and functional gene annotations in Indian salmon (Eleutheronema tetradactylum) through nextgeneration sequencing technology using illumina platform
Whole genome sequencing was performed on three samples of four finger threadfin Eleutheronema tetradactylum
(KET25, KET29 and KET30) using illumina NextSeq500 platform using 2×150 bp chemistry. 8,390,317,
7,085,775 and 8,461,589 high quality reads were obtained after trimming low quality reads and adapter sequence.
These high quality reads obtained were used for de novo assembly and obtained a number of scaffolds.
From these scaffolds of vast sequenced data, we were able to identify 60246, 46107 and 60907 Simple Sequence
Repeats (SSR) markers in KET25, KET29 and KET30 respectively, which will be useful in population genetic
analysis and other diversity studies in Indian salmon. The gene prediction on assembled scaffolds predicted
31,943 genes for KET25; 26,487 genes for KET29 and 31,654 genes for KET30 with average gene size of 458bp,
424bp and 459bp respectively. A total of 30,209, 25,107 and 29,943 genes were annotated against the NCBI Nr
database for the samples respectively. E. tetradactylum is a commercially important fish species for many
countries. This is the first report on the identification of genomic SSR markers in E. tetradactylum using NGS
technology. This study provides an insight of baseline knowledge of the genome sequence of Indian salmon for
future studies
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