Mechanical On-Chip Microwave Circulator

Nonreciprocal circuit elements form an integral part of modern measurement and communication systems. Mathematically they require breaking of time-reversal symmetry, typically achieved using magnetic materials and more recently using the quantum Hall effect, parametric permittivity modulation or Josephson nonlinearities. Here, we demonstrate an on-chip magnetic-free circulator based on reservoir engineered optomechanical interactions. Directional circulation is achieved with controlled phase-sensitive interference of six distinct electro-mechanical signal conversion paths. The presented circulator is compact, its silicon-on-insulator platform is compatible with both superconducting qubits and silicon photonics, and its noise performance is close to the quantum limit. With a high dynamic range, a tunable bandwidth of up to 30 MHz and an in-situ reconfigurability as beam splitter or wavelength converter, it could pave the way for superconducting qubit processors with integrated and multiplexed on-chip signal processing and readout.Comment: References have been update

Stationary Entangled Radiation from Micromechanical Motion

Mechanical systems facilitate the development of a new generation of hybrid quantum technology comprising electrical, optical, atomic and acoustic degrees of freedom. Entanglement is the essential resource that defines this new paradigm of quantum enabled devices. Continuous variable (CV) entangled fields, known as Einstein-Podolsky-Rosen (EPR) states, are spatially separated two-mode squeezed states that can be used to implement quantum teleportation and quantum communication. In the optical domain, EPR states are typically generated using nondegenerate optical amplifiers and at microwave frequencies Josephson circuits can serve as a nonlinear medium. It is an outstanding goal to deterministically generate and distribute entangled states with a mechanical oscillator. Here we observe stationary emission of path-entangled microwave radiation from a parametrically driven 30 micrometer long silicon nanostring oscillator, squeezing the joint field operators of two thermal modes by 3.40(37) dB below the vacuum level. This mechanical system correlates up to 50 photons/s/Hz giving rise to a quantum discord that is robust with respect to microwave noise. Such generalized quantum correlations of separable states are important for quantum enhanced detection and provide direct evidence for the non-classical nature of the mechanical oscillator without directly measuring its state. This noninvasive measurement scheme allows to infer information about otherwise inaccessible objects with potential implications in sensing, open system dynamics and fundamental tests of quantum gravity. In the near future, similar on-chip devices can be used to entangle subsystems on vastly different energy scales such as microwave and optical photons.Comment: 13 pages, 5 figure

The stochastic quantization method and its application to the numerical simulation of volcanic conduit dynamics under random conditions

Stochastic Quantization (SQ) is a method for the approximation of a continuous probability distribution with a discrete one. The proposal made in this paper is to apply this technique to reduce the number of numerical simulations for systems with uncertain inputs, when estimates of the output distribution are needed. This question is relevant in volcanology, where realistic simulations are very expensive and uncertainty is always present. We show the results of a benchmark test based on a one-dimensional steady model of magma flow in a volcanic conduit

Wave Height Attenuation and Flow Resistance Due to Emergent or Near-Emergent Vegetation.

Vegetation plays a pivotal role in fluvial and coastal flows, affecting their structure and turbulence, thus having a strong impact on the processes of transport and diffusion of nutrients and sediments, as well as on ecosystems and habitats. In the present experimental study, the attenuation of regular waves propagating in a channel through flexible vegetation is investigated. Specifically, artificial plants mimicking Spartina maritima are considered. Different plant densities and arrangements are tested, as well as different submergence ratios. Measurements of wave characteristics by six wave gauges, distributed all along the vegetated stretch, allow us to estimate the wave energy dissipation. The flow resistance opposed by vegetation is inferred by considering that drag and dissipation coefficients are strictly related. The submergence ratio and the stem density, rather than the wave characteristics, affect the drag coefficient the most. A comparison with the results obtained in the case when the same vegetation is placed in a uniform flow is also shown. It confirms that the drag coefficient for the canopy is lower than for an isolated cylinder, even if the reduction is not affected by the stem density, underlining that flow unsteadiness might be crucial in the process of dissipation

In Vitro and Ex Vivo Hemodynamic Testing of an Innovative Occluder for Paravalvular Leak After Transcather Aortic Valve Implantation

This study aims at achieving a proof-of-concept for a novel device designed to occlude the orifices that may form between transcatheter valves and host tissues after TAVI. The device effect on the performance of a SAPIEN XT with a paravalvular gap was assessed into an in vitro and ex vivo pulse duplicator. The in vitro tests were performed complying with the standard international regulations, measuring the trasvalvular pressure and regurgitant volumes with and without the paravalvular gap, and with the occluder correctly positioned into the gap. In the second series of tests, the leakage reduction due to the presence of the occluder was assessed for the same setup, into a beating swine heart. The occluder implantation decreased the regurgitant fraction of about 50% for the in vitro assessment and 75% for the ex vivo test, under rest operating conditions. These results suggest that suitably designed occluders can lead to important benefit in the PVL treatment

In vitro assessment of pacing as therapy for aortic regurgitation

Background and objective Clinical evaluation of pacing therapy in mitigating the aortic insufficiency after transchateter aortic valve implantation often gives contradictory outcomes. This study presents an in vitro investigation aimed at clarifying the effect of pacing on paravalvular leakage. Methods A series of in vitro tests reproducing the heart operating changes clinically obtained by pacing was carried out in a 26 mm Edwards Sapien XT prosthesis with mild paravalvular leakage. The effect of pacing on the regurgitant volumes per cycle and per minute was quantified, and the energy and power consumed by the left ventricle were calculated. Results Results indicate that though pacing results in some reduction in the total regurgitation per cycle, the volume of fluid regurgitating per minute increases substantially, causing overload of left ventricle. Conclusions Our tests indicate no effective haemodynamic benefit from pacing, suggesting a prudential clinical use of this therapy for the treatment of postoperative aortic regurgitation

Crispr/cas9 editing for gaucher disease modelling

Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the acid \u3b2-glucosidase gene (GBA1). Besides causing GD, GBA1 mutations constitute the main genetic risk factor for developing Parkinson\u2019s disease. The molecular basis of neurological manifestations in GD remain elusive. However, neuroinflammation has been proposed as a key player in this process. We exploited CRISPR/Cas9 technology to edit GBA1 in the human monocytic THP-1 cell line to develop an isogenic GD model of monocytes and in glioblastoma U87 cell lines to generate an isogenic GD model of glial cells. Both edited (GBA1 mutant) cell lines presented low levels of mutant acid \u3b2-glucosidase expression, less than 1% of residual activity and massive accumulation of substrate. Moreover, U87 GBA1 mutant cells showed that the mutant enzyme was retained in the ER and subjected to proteasomal degradation, triggering unfolded protein response (UPR). U87 GBA1 mutant cells displayed an increased production of interleukin-1\u3b2, both with and without inflammosome activation, \u3b1-syn accumulation and a higher rate of cell death in comparison with wild-type cells. In conclusion, we developed reliable, isogenic, and easy-to-handle cellular models of GD obtained from commercially accessible cells to be employed in GD pathophysiology studies and high-throughput drug screenings

In vitro haemodynamic testing of Amplatzer plugs for paravalvular leak occlusion after Transcather Aortic Valve Implantation

Objective: We aimed to in-vitro test Amplatzer devices (Amplatzer Vascular Plug II and Amplatzer Vascular Plug III, SJM St. Paul, MN) in closing PVL generated by transcatheter balloon expandable aortic valve prosthesis in order to quantify the effective treatment of PVL. Background: Transcatheter aortic valve replacement (TAVI) procedures represent the treatment of choice for high risk patients. Despite evolving technologies paravalvular leak (PVL) is still a major unaddressed issue. This severe complication significantly impair long-term survival. Percutaneous treatment of this complication is usually performed with the implantation of not specifically designed and not approved vascular devices. Methods: A 23mm Sapien XT (Edwards Lifesciences, Irvine, CA) was implanted in a rubber aortic root and a semi-elliptical shape PVL was created. The vascular occluder devices were implanted in the PVL and hemodynamic performance was tested in a pulse duplicator according to international standard ISO 5840-3:2013. Different type of comparison tests together with high speed camera recording allowed us to define the global efficiency of the occluders and their interaction with the transcatheter prosthesis. Results: The results revealed that the use of vascular plugs was not per se sufficient to produce an effective or substantial reduction of PVL with a maximum efficiency inferior of 50%. Recorded video showed clearly that the vascular plug always interfered with the leaflet of the prosthetic valve. Conclusions: Current used devices do not guarantee effective treatment of PVL and may otherwise compromise the structural integrity of the prosthetic valve implanted. Specific designed devices are required