Evaluation of the level of orientation of students sports training areas for a healthy lifestyle

The article presents the results of the study of the orientation of students sports training areas for a healthy lifestyle. Set this level of reduction for the period of training in high schoolПриводятся результаты изучения уровня ориентации студентов физкультурного направления подготовки на здоровый стиль жизни. Установлено снижение данного уровня за период обучения в вуз

Problem formation of the healthy lifestyle of students

In this article the role of a healthy lifestyle at students, need of his formation is considered, theoretical approaches to a problem of formation of a healthy lifestyle of students are revealedВ данной статье рассмотрена роль здорового образа жизни у студентов, необходимость его формирования, выявлены теоретические подходы к проблеме формирования здорового образа жизни студенто

The role of physical culture in personality formation

The article considers the concept of physical culture as a factor influencing the development of the individual identified theoretical approaches to the problem of moral education at the lessons of physical cultureВ статье рассмотрено понятие физической культуры как фактора, влияющего на развитие личности, выявлены теоретические подходы к проблеме нравственного воспитания на занятиях физической культуро

Orientation of students sports training areas for a healthy lifestyle

The article presents the results of the study of the orientation of students sports training areas for a healthy lifestyle. Set this level of reduction for the period of training in high schoolВ статье приводятся результаты изучения уровня ориентации студентов физкультурного направления подготовки на здоровый стиль жизни. Установлено снижение данного уровня за период обучения в вуз

The cytotoxicity of fatty acid/α-lactalbumin complexes depends on the amount and type of fatty acid

peer-reviewedComplexes of the milk protein, α-lactalbumin, and the fatty acid, oleic acid, have previously been shown to be cytotoxic. Complexes of α-lactalbumin and five different fatty acids (vaccenic, linoleic, palmitoleic, stearic, and elaidic acid) were prepared and compared to those formed with oleic acid. All complexes were cytotoxic to human promyelocytic leukemia-derived (HL-60) cells but to different degrees depending on the fatty acid. The amount of fatty acid per α-lactalbumin molecule was found to correlate with the cytotoxicity; the higher the number of fatty acids per protein, the more cytotoxic the complex. Importantly, all the tested fatty acids were also found to be cytotoxic on their own in a concentration dependent manner. The cytotoxic effect of complexes between α-lactalbumin and linoleic acid, vaccenic acid, or oleic acid was further investigated using flow cytometry and found to induce cell death resembling apoptosis on Jurkat cells. Practical applications: Cytotoxic complexes of α-lactalbumin and several different fatty acids could be produced. The cytotoxicity of all the variants is similar to that previously determined for α-lactalbumin/oleic acid complexes.This work was supported by the Food Institutional Research Measure (FIRM, project no. 08RDTMFRC650) of the Department of Agriculture, Fisheries, and Food, Ireland

Human α-fetoprotein as a Zn2+-binding protein. Tight cation binding is not accompanied by global changes in protein structure and stability

AbstractThe binding of zinc to human α-fetoprotein (AFP) isolated from human umbilical cord serum was studied by fluorimetric Zn2+-titration. We found that the total number of strong binding sites for zinc on this protein was 5: AFP has one very strong (dissociation constant, Kd<10−8 M) and at least four lower affinity zinc binding sites (Kd<10−5 M). Fourier transform infrared (FTIR) analysis revealed that aspartate and histidine residues could be involved in the strong coordination of zinc. Intriguingly, binding of zinc to the protein does not induce structural changes that can be detected by circular dichroism, FTIR, intrinsic fluorescence or (1,1′)-bi-(4-anilino)naphthalene-5,5′-disulfonic acid (bis-ANS) binding. Finally, scanning microcalorimetry measurements showed that stability of the protein is also unaffected by zinc binding in spite of the strength of the coordination. Such strong interactions without major structural consequences are highly unusual, and AFP may therefore be the first characterized representative of a new class of ligand-binding proteins