International laboratory comparison of influenza microneutralization assays for A(H1N1)pdm09, A(H3N2), and A(H5N1) influenza viruses by CONSISE

The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within protocols there are often further adjustments in individual laboratories. The impact these protocol variations have on influenza serology data is unclear. Thus, a laboratory comparison of the 2-day enzyme-linked immunosorbent assay (ELISA) and 3-day hemagglutination (HA) microneutralization (MN) protocols, using A(H1N1)pdm09, A(H3N2), and A(H5N1) viruses, was performed by the CONSISE Laboratory Working Group. Individual laboratories performed both assay protocols, on multiple occasions, using different serum panels. Thirteen laboratories from around the world participated. Within each laboratory, serum sample titers for the different assay protocols were compared between assays to determine the sensitivity of each assay and were compared between replicates to assess the reproducibility of each protocol for each laboratory. There was good correlation of the results obtained using the two assay protocols in most laboratories, indicating that these assays may be interchangeable for detecting antibodies to the influenza A viruses included in this study. Importantly, participating laboratories have aligned their methodologies to the CONSISE consensus 2-day ELISA and 3-day HAMNassay protocols to enable better correlation of these assays in the future

Frigophobia: a case series from Sri Lanka

Frigophobia is a condition in which patients report coldness of extremities leading to a morbid fear of death. It has been reported as a rare culture-related psychiatric syndrome in Chinese populations. An extensive survey of the literature yielded only six case reports. The present paper describes a series of 109 patients with frigophobia in Sri Lanka. The common clinical presentation was of patients initially examining their extremities for cold sensations, and then, with the onset of fear, covering themselves in layers of clothing, applying emollients, and staying near an open fire in an effort to ward off the cold. They avoided foods considered to be "cooling" and bathed only in the heat of the noonday sun. When the severity of the symptoms reached a peak, or when they felt death was imminent, Western medical aid was sought. The fear of dying is seen as the single most important aspect that drives these individuals to seek help. There was a preponderance of female patients, and a few had specific phobias as comorbid conditions. Management comprised primarily illness education, reassurance, and desensitisation by exposure to cold stimuli, with short-term anxiolytic medication use when necessary

Epstein-barr virus shedding and cytokine alterations in the oropharynxduring HIV-1 infection

published_or_final_versionDentistryDoctoralDoctor of Philosoph

JNDS, Volume 27, Number 4

Letter from Mahendra Perera to the editor discussing the deeds of Jesus Christ between his death and resurrection as near-death experiences

JNDS, Volume 24, Number 2

Study assessing the prevalence of near-death experiences (NDEs) in Australia through the use of questionnaires

JNDS, Volume 26, Number 4

Study exploring near-death experiences (NDEs) among suicide attempters, using interviews of 77 consecutive suicide attempters admitted to a tertiary teaching hospital in Sri Lanka