Simulation of Transport and Gain in Quantum Cascade Lasers

Quantum cascade lasers can be modeled within a hierarchy of different approaches: Standard rate equations for the electron densities in the levels, semiclassical Boltzmann equation for the microscopic distribution functions, and quantum kinetics including the coherent evolution between the states. Here we present a quantum transport approach based on nonequilibrium Green functions. This allows for quantitative simulations of the transport and optical gain of the device. The division of the current density in two terms shows that semiclassical transitions are likely to dominate the transport for the prototype device of Sirtori et al. but not for a recent THz-laser with only a few layers per period. The many particle effects are extremely dependent on the design of the heterostructure, and for the case considered here, inclusion of electron-electron interaction at the Hartree Fock level, provides a sizable change in absorption but imparts only a minor shift of the gain peak.Comment: 12 pages, 5 figures included, to appear in in "Advances in Solid State Physics", ed. by B. Kramer (Springer 2003

Simulations of mid infrared emission of InAsN semiconductors

This paper delivers an approximation to the complex many body problem of luminescence in semiconductors to the case of mid infrared luminescence of dilute nitrides. The results are compared with recent experimental data for InAsN semiconductors

Extrinsic Curvature and the Einstein Constraints

The Einstein initial-value equations in the extrinsic curvature (Hamiltonian) representation and conformal thin sandwich (Lagrangian) representation are brought into complete conformity by the use of a decomposition of symmetric tensors which involves a weight function. In stationary spacetimes, there is a natural choice of the weight function such that the transverse traceless part of the extrinsic curvature (or canonical momentum) vanishes.Comment: 8 pages, no figures; added new section; significant polishing of tex

Sistemas aquosos bifásicos uma ferramenta sustentável para a extração de ácido clavulânico a partir de diferentes fontes

O ácido clavulânico (AC) é um inibidor de β-lactamases que tem vindo a ser largamente utilizado na área médica. Embora seja de extrema importância, o desenvolvimento de processos alternativos de produção e purificação é ainda insignificante, sendo fundamental o estudo de técnicas de extração mais biocompatíveis, como os Sistemas Aquosos Bifásicos (SABs). Assim, este trabalho objetivou o estudo de Sistemas Aquosos Bifásicos baseados em polímeros como uma ferramenta alternativa para a extração de AC. Foram testados dois SPAB compostos por Polietileno Glicol (PEG) com massa molecular (M) de 4000 g/mol e Poliacrilato de Sódio de 8000 g/mol, nos quais foi alterado o eletrólito indutor da formação de fases, em particular, sulfato de sódio (Na2SO4,) e cloreto de sódio (NaCl). Ademais, este trabalho visou também avaliar a eficiência de extração do AC, bem como compreender o efeito dos contaminantes no processo de migração. Para tal, foi avaliada a extração do AC a partir de três fontes distintas: solução pura (99,9%); solução comercial (60%); diretamente a partir do sobrenadante de um meio fermentando de Streptomyces clavuligerus. Os resultados obtidos demonstraram que independentemente da fonte inicial do AC, ambos os SABs poliméricos promoveram uma partição preferencial do AC para a fase rica em PEG, sendo o coeficiente de partição maior nos sistemas com Na2SO4 do que com NaCl. Após identificar a grande capacidade de partição de AC, o SAB com PEG/NaPA/Na2SO4 foi também utilizado para avaliar a partição de proteínas presente no meio fermentado, sendo também obtida uma preferencial partição destas para a fase rica em PEG. Assim, apesar da baixa capacidade de purificação de AC frente a proteínas contaminantes, os SABs estudados demonstraram que podem ser uma técnica alternativa sustentável e bastante econômica para uma etapa inicial de clarificação/concentração de bioprodutos a partir de caldos fermentados

Gain in quantum cascade lasers and superlattices: A quantum transport theory

Gain in current-driven semiconductor heterostructure devices is calculated within the theory of nonequilibrium Green functions. In order to treat the nonequilibrium distribution self-consistently the full two-time structure of the theory is employed without relying on any sort of Kadanoff-Baym Ansatz. The results are independent of the choice of the electromagnetic field if the variation of the self-energy is taken into account. Excellent quantitative agreement is obtained with the experimental gain spectrum of a quantum cascade laser. Calculations for semiconductor superlattices show that the simple 2-time miniband transport model gives reliable results for large miniband widths at room temperatureComment: 8 Pages, 4 Figures directly included, to appear in Physical Review

Improved methods for detection of β-galactosidase (lacZ) activity in hard tissue

The ß-galactosidase gene (lacZ) of Escherichia coli is widely used as a reporter gene. The expression of lacZ can be detected by enzyme-based histochemical staining using chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-ß-D: -galactoside (X-gal). Because the enzymatic activity of lacZ is vulnerable to high temperatures and acid treatment for demineralization, detection of lacZ on paraffinized sections is difficult, especially for hard tissues, which require demineralization before sectioning in paraffin. To circumvent this problem, whole-mount X-gal staining before sectioning is performed. However, detection of lacZ activity in the center of larger portions of hard whole adult tissues is challenging. In this study, focusing on fixation procedures, we determined the conditions conducive to improved detection of lacZ activity in deeper areas of whole tissues. We used an annexin a5 (Anxa5)-lacZ reporter mouse model in which the Anxa5 expression in hard tissue is indicated by lacZ activity. We found that lacZ activity could be detected throughout the periodontal ligament of adult mice when fixed in 100% acetone, whereas it was not detected in the periodontal ligament around the root apex fixed in glutaraldehyde and paraformaldehyde. This staining could not be detected in wild-type mice. Acetone maintains the lacZ activity within 48 h of fixation at both 4°C and at room temperature. In conclusion, acetone is the optimal fixative to improve permeability for staining of lacZ activity in large volumes of adult hard tissues

Effects of acute aerobic exercise on rats serum extracellular vesicles diameter, concentration and small RNAs content

Physical exercise stimulates organs, mainly the skeletal muscle, to release a broad range of molecules, recently dubbed exerkines. Among them, RNAs, such as miRNAs, piRNAs, and tRNAs loaded in extracellular vesicles (EVs) have the potential to play a significant role in the way muscle and other organs communicate to translate exercise into health. Low, moderate and high intensity treadmill protocols were applied to rat groups, aiming to investigate the impact of exercise on serum EVs and their associated small RNA molecules. Transmission electron microscopy, resistive pulse sensing, and western blotting were used to investigate EVs morphology, size distribution, concentration and EVs marker proteins. Small RNA libraries from EVs RNA were sequenced. Exercise did not change EVs size, while increased EVs concentration. Twelve miRNAs were found differentially expressed after exercise: rno-miR-128-3p, 1033p, 330-5p, 148a-3p, 191a-5p, 10b-5p, 93-5p, 25-3p, 142-5p, 3068-3p, 142-3p, and 410-3p. No piRNA was found differentially expressed, and one tRNA, trna8336, was found down-regulated after exercise. The differentially expressed miRNAs were predicted to target genes involved in the MAPK pathway. A single bout of exercise impacts EVs and their small RNA load, reinforcing the need for a more detailed investigation into EVs and their load as mediators of health-promoting exercise

Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley.

The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 x 105 cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production

Use of Limpograss as an Alternative Feed During the Fall Forage Gap in Beef and Dairy Systems in Central and North Florida

In central and North Florida, the use of limpograss [Hemarthria altissima (Poir.) Stapf & C. E. Hubb.] for beef and dairy operations is limited and its potential use is not well documented. Two on-farm projects have been conducted in Central and North Florida to explore the use of limpograss as an alternative conserved forage during late fall and winter. The potential use of this forage as baleage for dairy farmers and as stockpiling for livestock producers would offer another alternative to reduce feed costs and fill the forage gap in the area when typical warm-season forages go dormant. In addition, the on-farm limpograss establishment would serve as dissemination for the limpograss planting material. Four dairy farms in Central and North Florida were enrolled in the study to evaluate two cultivars of limpograss for their potential when conserved as baleage. Four 0.2 ha strips were planted per farm, two for each variety (‘Kenhy’ and ‘Gibtuck’). The strips were arranged in a randomized complete block design, with two replicates in each location. Before wrapping the harvested forage for baleage, samples were taken to evaluate crude protein (CP) and in vitro digestible organic matter concentration (IVDOM). In addition, samples of fresh baleage at 60 and 90 d were analyzed for fermentation profile (pH, organic acids, and ammonia). Four beef cattle farms in North Florida allowed us to plant 1 ha of ‘Gibtuck’ for stockpiling evaluating the nutritive value at 30, 60, 90, and 120 days. Each plot was replicated four times and allocated in a randomized complete block design. The fermentation profile from the bales does not show differences between cultivars (P \u3e 0.05) and the pH is lower than 5 indicating that the fermentation process was successful. The CP and IVDOM of the stockpiling limpograss were different among the treatments (P \u3c 0.001)