A simple laser system for atom interferometry

We present here a simple laser system for a laser cooled atom interferometer, where all functions (laser cooling, interferometry and detection) are realized using only two extended cavity laser diodes, amplified by a common tapered amplifier. One laser is locked by frequency modulation transfer spectroscopy, the other being phase locked with an offset frequency determined by an Field-Programmable Gate Array (FPGA) controlled Direct Digital Synthesizer (DDS), which allows for efficient and versatile tuning of the laser frequency. Raman lasers are obtained with a double pass acousto-optic modulator. We demonstrate a gravimeter using this laser system, with performances close to the state of the art

Hybridizing matter-wave and classical accelerometers

We demonstrate a hybrid accelerometer that benefits from the advantages of both conventional and atomic sensors in terms of bandwidth (DC to 430 Hz) and long term stability. First, the use of a real time correction of the atom interferometer phase by the signal from the classical accelerometer enables to run it at best performances without any isolation platform. Second, a servo-lock of the DC component of the conventional sensor output signal by the atomic one realizes a hybrid sensor. This method paves the way for applications in geophysics and in inertial navigation as it overcomes the main limitation of atomic accelerometers, namely the dead times between consecutive measurements

Proteomic characterization of Benzalkonium Chloride- and Ciprofloxacin-adapted Pseudomonas aeruginosa biofilms

Bacteria are able to adapt to several environmental stresses such as the presence of antimicrobial molecules and, as consequence, bacterial resistance may increase with increasing exposure to antimicrobials. The most impressive mechanism of the bacterial mode of life is their grow as part of a sessile community referred to as biofilm [1]. Biofilm formation is an important aspect of many bacterial diseases, especially those related with medical devices [2]. When biofilms are identified as the cause of infection, treatment becomes very difficult since bacteria within biofilms demonstrate peculiar features, that confer them increased resistance to biocides. The adaptive response to antimicrobial stresses of sessile bacteria is more effective than the corresponding planktonic populations. Adaptive resistance to antimicrobials has been widely reported in planktonic state and characterized in terms of phenotypic traits and proteomic analysis [3,4]. Concerning biofilm adaptation, the response of the biofilm-entrapped cells to chemical stress conditions is not yet well studied. This work aimed to examine whether exposure of Pseudomonas aeruginosa biofilms to benzalkonium chloride (BC) and ciprofloxacin (CIP) during a laboratory adaptation process could induce any proteomic alterations in the outer membrane (OM) of the biofilm cells. Biofilms were formed in 6-well plates for 24 h being after submitted to the presence of 324 mg/L of BC and 6.0 mg/L of CIP, during 13 days. The obtained biofilm-cells were separated from the biofilm matrix and the OM proteins extracted. Protein patterns were analyzed by 2-DE and gels by Progenesis SameSpot software. Protein spots from the bacterial populations were considered to display significant quantitative differences if they fulfilled the following criteria: p values ≤ 0.05 (t-test); detection threshold, average volume ≥ 20 (n = 3); differential tolerance, fold change ≥ 2. Excised spots from three different gels of each adapted bacteria were identified by LC-MS/MS. Biofilm proteome analysis showed that P. aeruginosa adaptation to BC and CIP changed the expression of six proteins. The biofilm exposure to both antimicrobials generated common down-regulation of three proteins: GroEL, major capsid protein and putative tail sheath protein, revealing a possible similar stress response. The type 4 fimbrial biogenesis outer membrane protein PilQ precursor was over-expressed only in biofilms submitted to BC, while the probable bacteriophage protein and the hypothetical protein PA0537 were overexpressed in CIP exposed biofilms. When bacteria are within biofilms and exposed to chemical stress, the regulation of OM proteins expression can contribute to increase the biofilm resistance. The proteins involved in adhesion, oxidative stress response, as well as in synthesis of lipopolysaccharide, were upor down-regulated in adapted P. aruginosa biofilms. These acquired proteomic profiles may be associated with antimicrobial tolerance

Employment of Free Packages for MT-InSAR Approaches to Verify the Subsidence Event over Maceió City, Brazil

Persistent Scatterer Interferometric Synthetic Aperture Radar (PSInSAR) technique employs a Multi-Temporal InSAR (MT-InSAR) approach to accurately measure subsidence. This technique, a type of Differential Interferometry (DInSAR), mitigates errors that traditional DInSAR techniques cannot, including temporal and geometric decorrelation, and phase unwrapping errors. In order to verify the subsidence process in the Pinheiro neighborhood of Maceio - Brazil following a 2018 earthquake, we tested free processing packages such as SNAP-StaMPS integration. Our investigation was conducted in two stages: first, using a stack of Sentinel-1A SLCSAR (Single Look Complex-SAR) images acquired before and after the earthquake, and second, using more recent images to determine if the subsidence process is ongoing. Results from the first stage identified the area affected by subsidence and the second stage confirmed the continued presence of subsidence events. From 2017 to 2018, the subsidence process exhibited the highest displacement amplitude of -32.3 mm/year, whereas, between 2021 and 2022, the amplitude decreased to -24.09 mm/year, indicating a deceleration in the subsidence process

Effects of UV-organic interaction and Martian conditions on the survivability of organics

This work was funded by the Leverhulme Trust (RPG-2015-071). Dr. C. Cousins also wishes to acknowledge funding from the Royal Society of Edinburgh. This work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-029932 (PTDC/FIS-AST/29932/2017).Exogenous organic molecules are delivered to the surface of Mars annually, yet their fate is largely unknown. Likewise, the survivability of putative organic biomarkers directly implicates current Mars surface exploration ambitions. Among these, amino acids are valuable target molecules due to their abiogenic and biological origins. We present the fundamental, but not previously considered, factors that affect the fate of amino acids embedded in Mars mineral analogues when exposed to ionising radiation. Using existing experimental datasets, we show that the attenuation coefficient at 200 nm for amino acids is an effective parameter for quantifying organic survivability, especially when mineral shielding is limited or absent. Conversely, the dielectric constant of a material is a potential key parameter regarding mineral shielding, as it accounts for iron content, and the physical properties of the material (pore size, surface area or water content). Finally, we combine Martian climatic parameters (surface temperature and atmospheric opacity) to show that the relative UV environment varies significantly on Mars as a function of latitude, providing a reference point for future Mars simulation studies.PostprintPeer reviewe

Silicon nanowires as negative electrode for lithium-ion microbatteries

International audienceThe increasingly demand on secondary batteries with higher specific energy densities requires the replace- ment of the actual electrode materials. With a very high theoretical capacity (4200 mAh g−1 ) at low voltage, silicon is presented as a very interesting potential candidate as negative electrode for lithium-ion micro- batteries. For the first time, the electrochemical lithium alloying/de-alloying process is proven to occur, respectively, at 0.15 V/0.45 V vs. Li+ /Li with Si nanowires (SiNWs, 200-300 nm in diameter) synthesized by chemical vapour deposition. This new three-dimensional architecture material is well suited to accom- modate the expected large volume expansion due to the reversible formation of Li-Si alloys. At present, stable capacity over ten to twenty cycles is demonstrated. The storage capacity is shown to increase with the growth temperature by a factor 3 as the temperature varies from 525 to 575 ◦ C. These results, showing an attractive working potential and large storage capacities, open up a new promising field of research

CTG Trinucleotide Repeat “Big Jumps”: Large Expansions, Small Mice

Trinucleotide repeat expansions are the genetic cause of numerous human diseases, including fragile X mental retardation, Huntington disease, and myotonic dystrophy type 1. Disease severity and age of onset are critically linked to expansion size. Previous mouse models of repeat instability have not recreated large intergenerational expansions (“big jumps”), observed when the repeat is transmitted from one generation to the next, and have never attained the very large tract lengths possible in humans. Here, we describe dramatic intergenerational CTG•CAG repeat expansions of several hundred repeats in a transgenic mouse model of myotonic dystrophy type 1, resulting in increasingly severe phenotypic and molecular abnormalities. Homozygous mice carrying over 700 trinucleotide repeats on both alleles display severely reduced body size and splicing abnormalities, notably in the central nervous system. Our findings demonstrate that large intergenerational trinucleotide repeat expansions can be recreated in mice, and endorse the use of transgenic mouse models to refine our understanding of triplet repeat expansion and the resulting pathogenesis

Discovery of optical pulsations in V2116 Ophiuchi/GX 1+4

We report the detection of pulsations with $\sim 124$ s period in V2116 Oph, the optical counterpart of the low-mass X-ray binary GX 1+4. The pulsations are sinusoidal with modulation amplitude of up to 4% in blue light and were observed in ten different observing sessions during 1996 April-August using a CCD photometer at the 1.6-m and 0.6-m telescopes of Laborat\'orio Nacional de Astrof\'{\i}sica, in Brazil. The pulsations were also observed with the $UBVRI$ fast photometer. With only one exception the observed optical periods are consistent with those observed by the BATSE instrument on board the Compton Gamma Ray Observatory at the same epoch. There is a definite correlation between the observability of pulsations and the optical brightness of the system: V2116~Oph had $R$ magnitude in the range $15.3-15.5$ when the pulsed signal was detected, and $R = 16.0-17.7$ when no pulsations were present. The discovery makes GX 1+4 only the third of $\sim 35$ accretion-powered X-ray pulsars to be firmly detected as a pulsating source in the optical. The presence of flickering and pulsations in V2116 Oph adds strong evidence for an accretion disk scenario in this system. The absolute magnitude of the pulsed component on 1996 May 27 is estimated to be $M_V \sim -1.5$. The implied dimensions for the emitting region are 1.1 R_{\sun}, 3.2 R_{\sun}, and 7.0 R_{\sun}, for black-body spectral distributions with $T = 10^5$ K, $2 \times 10^4$ K, and $1 \times 10^4$ K, respectively.Comment: 9 pages, 3 figures in PostScript, latex, accepted for publication on the Astrophysical Journal Letter