Potential for macro and micronutrients extraction from tomato plants with different soil water stresses

Different tomato cultivars may present differentiated water needs, making it indispensable to study water demand. Thus, the objective of this work was to evaluate the influence of six water stresses in the soil on the extraction potential of macro and micronutrients in the aerial part of tomato in vegetative stage, cultivar ‘Dominador’ F1, under protected cultivation and drip. The experiment was installed in a greenhouse with a randomized block design with four replications. The treatments consisted of six soil water stresses as indicative of the time of irrigation. The preset stresses were 20, 45, 70, 95, 120 and 145 kPa at 20 cm depth. At 140 days after transplanting, the variables evaluated were: the macro and micronutrient content of shoots. The results showed that to obtain higher levels of macro (P and S) and micronutrients (B and Cu) of the total aerial part of the ‘Dominador’ tomato plant F1, it was obtained at a voltage of 20 kPa, and its value was reduced linearly with the increase of the water tension in the soil

Coping with suboptimal water temperature: modifications in blood parameters, body composition, and postingestive-driven diet selection in Nile tilapia fed two vegetable oil blends

The world tilapia production faces seasonal variations. However, very few nutritional studies have addressed suboptimal temperature. We evaluated the effect of two temperatures (20 or 30 °C) and two vegetable oil blends (one rich in corn oil (COR) and one rich linseed oil (LIN)) on tilapia growth, body composition, and blood parameters using a 2 × 2 factorial design with the following treatments: COR-20; LIN-20; COR-30; LIN-30 (Trial 1). In addition, we also evaluated the effect of postingestive signals of dietary oils when the organoleptic properties of diets were isolated (Trial 2). In the Trial 1, 256 fish (15.36 ± 0.14 g) were placed in 16 aquariums and submitted during 30 days to the 2 × 2 factorial designs: COR-20; LIN-20; COR-30; LIN-30. The temperatures were established in two independent water recirculation systems. In the Trial 2, 96 fish (34.02 ± 0.79 g) were placed in 12 aquariums and subjected to the same experimental design of Trial 1, but to evaluate fish feeding behavior. They were allowed to select the encapsulated diets provided in different feeding halls to evaluate if diet preferences are influenced by postingestive signals. As the Trial 1 results show, diets had no significant effects on growth, dietary protein use, and body centesimal composition, but 30 °C induced the best performance and protein deposition (P < 0.05). LIN-20 showed lower very-low-density lipoprotein and cortisol, but higher high-density lipoprotein (HDL), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and triglycerides (TG) than COR-20 (P < 0.05). COR-30 presented higher HDL, AST, ALT, TG, and cortisol than LIN-30. The fish fed COR showed lower C20:5n-3 (EPA) and higher n-6 than fish fed LIN (P < 0.05). The fish fed LIN had high n-3 highly unsaturated fatty acid. ∑ polyunsaturated fatty acid was higher at 30 °C. Finally, the tilapia in Trial 2 showed clear diet intake regulation and preference for LIN (P < 0.05), regardless of temperature. In short, lipid sources had no influence on tilapia performance; however, temperature affects carcass lipid deposition as well as fatty acids profile. Notably, the preference for linseed oil can suggest nutritional metabolic issues, contributing to animal behavior knowledge

Gene projects: a genome web tool for ongoing mining and annotation applied to CitEST

Genome projects, both genomic DNA and ESTs (cDNA), generate a large amount of information, demanding time and a well-structured bioinformatics laboratory to manage these data. These genome projects use information available in heterogeneous formats from different sources. The amount and heterogeneity of this information, as well as the absence of a world consensus pattern, make the integration of these data a difficult task. At the same time, sub-tasks, such as microarray analyses of these projects, are very complex. This creates a demand for the development of creative solutions for ongoing annotation, thematic projects, microarray experiments, etc. This paper presents Gene Projects, a system developed to integrate all kinds of solutions.10301036Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

Gastric content technique collection for in vitro degradation and gas production in horses supplemented with live yeast and protected yeast

ABSTRACT Due to the difficulty in acessing certain sites of fermentation and possible starch digestion, studies show the need for equine gastric digestive fisiology evaluation. The aim of this experiment was to assess horses supplemented with live yeast and protected live yeast (Saccharomyces cerevisae NCYC Sc 47) to validate a new procedure of gastric content collection for determination of pH, latic acid, short chain fatty acids and gas production and degradation to be used in further in vitro fermentative studies. The experimental design used was a the latin square (4x4) for 4 periods and 1 day of gastric content collection, with 15 days of rest between it. The groups were divided in control (CTRL), non protected live yeast (NPYEA), protected live yeast (PYEA) and a combination of live yeast + protected yeast (COMB). Treatment means were compared using orthogonal contrasts (C1: CTRL versus NPYEA, PYEA and COMB; C2: COMB versus NPYEA and PYEA; C3: NPYEA versus PYEA) and Tukey´s test was used at a 5% significance level. The procedure was highly tolerated and provided great accuracy of the collection site. The live yeast supplementation increased the concentration of acetic acid at the horses’ stomachs. When gastric content was used as inoculum for in vitro fermentation, the protected live yeast produced less gas

Star Models with Dark Energy

We have constructed star models consisting of four parts: (i) a homogeneous inner core with anisotropic pressure (ii) an infinitesimal thin shell separating the core and the envelope; (iii) an envelope of inhomogeneous density and isotropic pressure; (iv) an infinitesimal thin shell matching the envelope boundary and the exterior Schwarzschild spacetime. We have analyzed all the energy conditions for the core, envelope and the two thin shells. We have found that, in order to have static solutions, at least one of the regions must be constituted by dark energy. The results show that there is no physical reason to have a superior limit for the mass of these objects but for the ratio of mass and radius.Comment: 20 pages, 1 figure, references and some comments added, typos corrected, in press GR