PARP inhibition attenuates histopathological lesion in ischemia/reperfusion renal mouse model after cold prolonged ischemia

We test the hypothesis that PARP inhibition can decrease acute tubular necrosis (ATN) and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of Wisconsin (UW) solution. Material and Methods. We used 30 male Parp1+/+ wild-type and 15 male Parp10/0 knockout C57BL/6 mice. Fifteen of these wild-type mice were pretreated with 3,4-dihydro-5-[4-(1-piperidinyl)butoxyl]-1(2H)-isoquinolinone (DPQ) at a concentration of 15 mg/kg body weight, used as PARP inhibitor. Subgroups of mice were established (A: IR 45 min/6 h; B: IR + 48 h in UW solution; and C: IR + 48 h in UW solution plus DPQ). We processed samples for morphological, immunohistochemical, ultrastructural, and western-blotting studies. Results. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with PARP inhibitor DPQ plus DPQ supplementation in UW solution decreased PARP-1 nuclear expression in renal tubules and renal damage. Parp10/0 knockout mice were more resistant to IR-induced renal lesion. In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored organs at transplantation.Funding: This research was supported by CTS no. 138 Research Group and from the Carlos III Health Institute of the Spanish Ministery of Health and Consumer Affairs (Red de Investigación Renal, REDinREN 012/0021/0025). “FEDER una manera de hacer Europa”

Proyecto Factible para la Ampliaci\uf3n de la Organizaci\uf3n Berit C.A, en Servicios de Impresi\uf3n a Gran Formato o Gigantograf\ueda

En el presente estudio tiene como prop\uf3sito de elaborar un proyecto factible, estableciendo la planificaci\uf3n, control de recursos y actividades, que permitan las estrategias para un crecimiento y generar productividad, para la ampliaci\uf3n de la organizaci\uf3n BERIT CA, en servicios de impresi\uf3n a gran formato o gigantografia, de acuerdo a los procedimientos metodol\uf3gicos que sustentan una investigaci\uf3n cient\uedfica. El proyecto factible surge en respuesta a las necesidades individuales y colectivas, presenten en el mercado y en la organizaci\uf3n, donde procura ocasionar est\uedmulos en la demanda y oferta. El dise\uf1o de esta investigaci\uf3n de una investigaci\uf3n no experimental descriptivo, para la cual se aplico un instrumento tipo cuestionario compuesto de 10 preguntas cerradas aplicadas a una muestra de 80 organizaciones grandes y peque\uf1as del Municipio Jim\ue9nez, una entrevista aplicada a los competidores en el mercado, para luego ser analizadas y ser presentadas en una representaci\uf3n grafica tipo pastel, los resultados muestra la demanda existente de servicios gigantograficos en el mercado local, as\ued como su aceptaci\uf3n y la detecci\uf3n de clientes potenciales. El adoptar la tecnolog\ueda propuesta en el proyecto factible, \uedndice al incorporar innovaci\uf3n y creatividad en el mercado publicitario, produciendo mejoras y eficiencia en cuanto a la productividad, y a la rentabilidad. El estudio econ\uf3mico, presenta que el proyecto factible que requiere una inversi\uf3n, de Bs. F 209.885,00, con un financiamiento de Bs. 139.885 y la evaluaci\uf3n financiera permiti\uf3 conocer, que la propuesta tienen un VAN de Bs. F 329.783,82 y una TIR de 70,48%, lo que indica los beneficioso del proyecto, haci\ue9ndolo factible

Detection of Trypanosoma cruzi and Trypanosoma rangeli Infection by Duplex PCR Assay Based on Telomeric Sequences

We used the species specificity and repetitious nature of subtelomeric kinetoplastida sequences to generate a duplex PCR assay for the simultaneous detection of Trypanosoma cruzi and Trypanosoma rangeli in experimentally and naturally infected triatomine (Reduviid) bugs and in infected human subjects. The assay was species specific and was capable of detecting 1/20th of T. cruzi and 1/4th of T. rangeli cell equivalents without complementary hybridization. In addition, the PCR-based assay was robust enough for direct application to difficult biological samples such as Reduviid feces or guts and was capable of recognizing all T. cruzi and T. rangeli strains and lineages. Because the assay primers amplify entirely different target sequences, no reaction interference was observed, facilitating future adaptation of this assay to an automated format

Trypanosoma rangeli expresses a gene of the group II trans-sialidase superfamily

133–[email protected]@[email protected]@[email protected]

beta-Conglutins' mobile arm is crucial in the nutraceutical properties of blue lupin

Resumen de la comunicación oral presentada en ILS4 Fourth International Legume Society Conference. 19-22 Septiembre 2023. Granada, España

β-Conglutins’ Unique Mobile Arm Is a Key Structural Domain Involved in Molecular Nutraceutical Properties of Narrow-Leafed Lupin (<i>Lupinus angustifolius</i> L.)

Narrow-leafed lupin (NLL; Lupinus angustifolius L.) has multiple nutraceutical properties that may result from unique structural features of β-conglutin proteins, such as the mobile arm at the N-terminal, a structural domain rich in α-helices. A similar domain has not been found in other vicilin proteins of legume species. We used affinity chromatography to purify recombinant complete and truncated (without the mobile arm domain, tβ5 and tβ7) forms of NLL β5 and β7 conglutin proteins. We then used biochemical and molecular biology techniques in ex vivo and in vitro systems to evaluate their anti-inflammatory activity and antioxidant capacity. The complete β5 and β7 conglutin proteins decreased pro-inflammatory mediator levels (e.g., nitric oxide), mRNA expression levels (iNOS, TNFα, IL-1β), and the protein levels of pro-inflammatory cytokine TNF-α, interleukins (IL-1β, IL-2, IL-6, IL-8, IL-12, IL-17, IL-27), and other mediators (INFγ, MOP, S-TNF-R1/-R2, and TWEAK), and exerted a regulatory oxidative balance effect in cells as demonstrated in glutathione, catalase, and superoxide dismutase assays. The truncated tβ5 and tβ7 conglutin proteins did not have these molecular effects. These results suggest that β5 and β7 conglutins have potential as functional food components due to their anti-inflammatory and oxidative cell state regulatory properties, and that the mobile arm of NLL β-conglutin proteins is a key domain in the development of nutraceutical properties, making NLL β5 and β7 excellent innovative candidates as functional foods

Challenging Assumptions: "Unveiling Meritocracy's Reality in Neurosurgery"

Introduction: Meritocracy, a concept revered as the cornerstone of fairness and equal opportunity, is critically examined in the context of neurosurgery. This article challenges the notion that success in this demanding field is solely determined by individual abilities and effort. By investigating how these systemic barriers impact admissions to neurosurgical training programs and professional advancement, the paper underscores the complexity of meritocracy in neurosurgery, suggesting that the meritocratic ideal is more nuanced and influenced by external variables than commonly believed. Results: Certain universities deemed elite offer a curriculum divergent from that of their counterparts in low and middle-income countries. Students at these "elite" institutions gain exposure to new technologies and research incentives, which brings us to the realm of research. Remarkably, 75% of articles originating from developed nations account for just 25% of traumatic brain injury cases. This disparity highlights a significant research imbalance, and the common refrain underscores the need to bolster research capabilities in low-income countries. Neurosurgeons in the United States receive a median? salary of $412,000 dollars per year, compared to$13200 dollars in Latin America, as of June 2023. Given such incongruities, the prospect of even attending conferences or workshops abroad remains difficult for neurosurgeons from developing nations. Research isn't cast aside due to a lack of interest but due to resource. The narrative promotes a collective endeavour to dismantle barriers and embrace innovation, emphasizing the importance of mentorship, cross-institutional collaboration, and the amplification of underrepresented voices

Ilustración del proceso constructivo de vías primarias, secundarias y terciarias basado en las especificaciones técnicas de construcción de carreteras del Instituto Nacional de vías (INVIAS)

: figuras, tablas ; 28 cmEsta cartilla metodológica describe a través de ilustraciones el proceso constructivo de vías primarias, secundarias y terciarias, basado en las especificaciones técnicas de construcción de carreteras del Instituto Nacional de Vías (INVIAS)PregradoIngeniero CivilIngeniería Civi