Intruder bands and configuration mixing in the lead isotopes

A three-configuration mixing calculation is performed in the context of the interacting boson model with the aim to describe recently observed collective bands built on low-lying $0^+$ states in neutron-deficient lead isotopes. The configurations that are included correspond to the regular, spherical states as well as two-particle two-hole and four-particle four-hole excitations across the Z=82 shell gap.Comment: 20 pages, 4 figures, accepted by PRC, reference added for section 1 in this revised versio

Efficacious Intermittent Dosing of a Novel JAK2 Inhibitor in Mouse Models of Polycythemia Vera

A high percentage of patients with the myeloproliferative disorder polycythemia vera (PV) harbor a Val617→Phe activating mutation in the Janus kinase 2 (JAK2) gene, and both cell culture and mouse models have established a functional role for this mutation in the development of this disease. We describe the properties of MRLB-11055, a highly potent inhibitor of both the WT and V617F forms of JAK2, that has therapeutic efficacy in erythropoietin (EPO)-driven and JAK2V617F-driven mouse models of PV. In cultured cells, MRLB-11055 blocked proliferation and induced apoptosis in a manner consistent with JAK2 pathway inhibition. MRLB-11055 effectively prevented EPO-induced STAT5 activation in the peripheral blood of acutely dosed mice, and could prevent EPO-induced splenomegaly and erythrocytosis in chronically dosed mice. In a bone marrow reconstituted JAK2V617F-luciferase murine PV model, MRLB-11055 rapidly reduced the burden of JAK2V617F-expressing cells from both the spleen and the bone marrow. Using real-time in vivo imaging, we examined the kinetics of disease regression and resurgence, enabling the development of an intermittent dosing schedule that achieved significant reductions in both erythroid and myeloid populations with minimal impact on lymphoid cells. Our studies provide a rationale for the use of non-continuous treatment to provide optimal therapy for PV patients

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications

Cyclodextrin glucanotransferases (CGTases) are industrially important enzymes that produce cyclic α-(1,4)-linked oligosaccharides (cyclodextrins) from starch. Cyclodextrin glucanotransferases are also applied as catalysts in the synthesis of glycosylated molecules and can act as antistaling agents in the baking industry. To improve the performance of CGTases in these various applications, protein engineers are screening for CGTase variants with higher product yields, improved CD size specificity, etc. In this review, we focus on the strategies employed in obtaining CGTases with new or enhanced enzymatic capabilities by searching for new enzymes and improving existing enzymatic activities via protein engineering

Genereren van een 6-positie postcodebestand op basis van de kadastrale registratie

Accurate postal code maps have many applications within GIS as the postal code has the potential to link the address description of buildings to their location in a specified global reference system in a more natural way. This relationship is possible in both directions: geocoding and reverse-geocoding. These operators demand a mechanism for translating an exact geometric position (i.e., WGS84 coordinate) into a location indication (town, street, house number) and vice versa. As most built-up parcels are provided with a postal code, this indicator can be used as the linkage. This thesis describes the procedure, based on the Dutch cadastral registration, to obtain a reliable 6-position (i.e., 2628BX, the highest level of detail possible) planar postal code map for the Netherlands. Problems with existing, Voronoi-diagram based, postal code maps, like intersected houses and arbitrary derived (and thus unrecognizable) boundaries are avoided. The reliability of the derived planar postal code map is discussed and results are illustrated by figures. For a planar coverage, non built-up parcels having no postal code should be assigned a plausible postal code. Furthermore special attention is given to infrastructural parcels. These parcels are divided at their (approximated) skeletons first and then these subdivided infrastructure parcels are piecewise attached to their neighbour parcels. This new approach results in very reliable postal code maps, which are visually attractive too as infrastructure lines can be recognized. The procedure is generic and can be applied to other administrative parcel information as well. The algorithm is implemented using the Computational Geometry Algorithms Library (CGAL), and the possibilities and limitations of this library are addressed as well. Also a number of non-implemented alternatives or improvements are given.GIS technologyCivil Engineering and Geoscience

3D topography: A simplicial complex-based solution in a spatial DBMS

Current topographic products are usually limited to a real world representation in only two dimensions, extended with some additional point heights and contour lines. Many environmental issues require more precision than analysis on 2D models can offer. At the same time, vast amounts of 3D data are becoming available due to emerging new data acquisition techniques, such as (terrestrial) laser scanning. To extend current models into the third dimension, a data structure is needed to load, update, query, analyse and especially validate the new 3D topographic data in a spatial DBMS. This dissertation presents a new topological approach to 3D data modelling, based on simplicial complexes. Operators and definitions from the field of simplicial homology are used to define and handle the is tetrahedral network (TEN). Simplicial homology provides a solid mathematical foundation for the data structure. The new database approach enables one to derive substantial parts of the data structure efficiently, thus tackling common drawbacks as TEN extensiveness and laboriousness of maintaining topology.OT