On digital bioprocessing for manufacturing intelligence: Application of process analytical technology (PAT) and process data analytics (PDA) for upstream process development and intensification

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Impurity of Stem Cell Graft by Murine Embryonic Fibroblasts – Implications for Cell-Based Therapy of the Central Nervous System

Stem cells have been demonstrated to possess a therapeutic potential in experimental models of various central nervous system disorders, including stroke. The types of implanted cells appear to play a crucial role. Previously, groups of the stem cell network NRW implemented a feeder-based cell line within the scope of their projects, examining the implantation of stem cells after ischemic stroke and traumatic brain injury. Retrospective evaluation indicated the presence of spindle-shaped cells in several grafts implanted in injured animals, which indicated potential contamination by co-cultured feeder cells (murine embryonic fibroblasts – MEFs). Because feeder-based cell lines have been previously exposed to a justified criticism with regard to contamination by animal glycans, we aimed to evaluate the effects of stem cell/MEF co-transplantation. MEFs accounted for 5.3 ± 2.8% of all cells in the primary FACS-evaluated co-culture. Depending on the culture conditions and subsequent purification procedure, the MEF-fraction ranged from 0.9 to 9.9% of the cell suspensions in vitro. MEF survival and related formation of extracellular substances in vivo were observed after implantation into the uninjured rat brain. Impurity of the stem cell graft by MEFs interferes with translational strategies, which represents a threat to the potential recipient and may affect the graft microenvironment. The implications of these findings are critically discussed

results of a randomized controlled trial

Bile acids (BAs) are increasingly recognised as metabolic regulators, potentially improving insulin sensitivity following bariatric surgery. However, physiological relevance of such observations remains unknown. Hence, we analysed serum BA composition and associated gut-derived hormone levels following lifestyle-induced weight loss in individuals with metabolic syndrome (MetS). 74 non-smoking men (45–55 yr) with MetS were randomised to a lifestyle-induced weight loss program (supervision via telemonitoring) or to a control arm. Before and after a 6 months intervention period clinical and laboratory parameters, body composition, serum BA profile, FGF-19, and GLP-1 concentrations were determined in fasting blood samples. 30 participants in the control and 33 participants in the treatment arm completed the study and were included in the data analysis. In participants of the treatment arm lifestyle-induced weight loss resulted in markedly improved insulin sensitivity. Serum levels of BA species and total GLP-1 decreased, while FGF-19 remained stable. Serum BA composition changed towards an increased 12α- hydroxylated/non-12α-hydroxylated ratio. None of these parameters changed in participants of the control arm. Our results demonstrate that improved metabolic control by lifestyle modifications lowers serum levels of BAs and GLP-1 and changes serum BA composition towards an increased 12α/non-12α ratio (ICTRP Trial Number: U1111-1158-3672)

Flatland

Flatland is a project of VCDE233 TYPOGRAPHY II and VCDI223 DESIGN AND PRE-PRESS PRODUCTION, both courses in the Design Studies diploma program at MacEwan University. Students were asked to translate an assigned section of the Victorian novella, Flatland: A Romance of Many Dimensions by Edwin A. Abbott (1884), into a two-page layout that treats the text in a way that is visually appealing, readable, and appropriate to the content. They were encouraged to challenge conventions by exploring alternative grids, objective and expressive type, and text and image relationships. VCDE233 Typography II (Constanza Pacher) and VCDI223 Design and Pre-Press Production (Jess Dupuis

The bii4africa dataset of faunal and floral population intactness estimates across Africa’s major land uses

Sub-Saharan Africa is under-represented in global biodiversity datasets, particularly regarding the impact of land use on species’ population abundances. Drawing on recent advances in expert elicitation to ensure data consistency, 200 experts were convened using a modified-Delphi process to estimate ‘intactness scores’: the remaining proportion of an ‘intact’ reference population of a species group in a particular land use, on a scale from 0 (no remaining individuals) to 1 (same abundance as the reference) and, in rare cases, to 2 (populations that thrive in human-modified landscapes). The resulting bii4africa dataset contains intactness scores representing terrestrial vertebrates (tetrapods: ±5,400 amphibians, reptiles, birds, mammals) and vascular plants (±45,000 forbs, graminoids, trees, shrubs) in sub-Saharan Africa across the region’s major land uses (urban, cropland, rangeland, plantation, protected, etc.) and intensities (e.g., large-scale vs smallholder cropland). This dataset was co-produced as part of the Biodiversity Intactness Index for Africa Project. Additional uses include assessing ecosystem condition; rectifying geographic/ taxonomic biases in global biodiversity indicators and maps; and informing the Red List of Ecosystems

Shipborne And Ground-Based Lidar Monitoring of the Atmosphere Over the Lake Baikal in 2018

The paper presents the results of studies aimed at the analysis and assessment of atmospheric pollution over the Lake Baikal in the summer. This information is necessary to create physical models of the formation and transfer of atmospheric aerosol fields, taking into account the physical and geographical features of the Baikal region. Measurements were carried out by a lidar «LOSA-A2» installed on the scientific-research vessel «Academician V.A. Koptyug». The vessel’s route passed along the South, Middle and Northern Baikal, from July 15, 2018 to July 26, 2018. At the same time, observations were conducted using lidar «LOSA-M2». It was located in the background area at Boyarsky stationary site (51.84° N, 106.06° E), in the south-eastern part of the lake. The results of changes in the spatial structure of atmospheric aerosol fields in background conditions and during forest fires are shown

Shipborne And Ground-Based Lidar Monitoring of the Atmosphere Over the Lake Baikal in 2018

The paper presents the results of studies aimed at the analysis and assessment of atmospheric pollution over the Lake Baikal in the summer. This information is necessary to create physical models of the formation and transfer of atmospheric aerosol fields, taking into account the physical and geographical features of the Baikal region. Measurements were carried out by a lidar «LOSA-A2» installed on the scientific-research vessel «Academician V.A. Koptyug». The vessel’s route passed along the South, Middle and Northern Baikal, from July 15, 2018 to July 26, 2018. At the same time, observations were conducted using lidar «LOSA-M2». It was located in the background area at Boyarsky stationary site (51.84° N, 106.06° E), in the south-eastern part of the lake. The results of changes in the spatial structure of atmospheric aerosol fields in background conditions and during forest fires are shown

Observations of the Horizontally Oriented Crystalline Particles with a Scanning Polarization Lidar

Scanning lidar LOSA-M3 makes it possible to measure the polarization characteristics of backscattering signals from aerosol and clouds at wavelength 532 and 1064 nm. The lidar transceiver is placed on a scanning column, which allows changing the direction of sounding within the upper hemisphere at a speed of 1 degree per second. The polarization characteristics of the transmitter (linear or circular polarization) can be changed by rotating the phase plates synchronously with the laser pulses. Conical scanning of the lidar allows identifying cloud areas with preferential zenith or azimuthal orientation of the crystal particles. The article presents the results of observations of the cloud polarization structure carried out in Tomsk. Methods of the calibrations of lidar polarization channels are described

Observations of the Horizontally Oriented Crystalline Particles with a Scanning Polarization Lidar

Scanning lidar LOSA-M3 makes it possible to measure the polarization characteristics of backscattering signals from aerosol and clouds at wavelength 532 and 1064 nm. The lidar transceiver is placed on a scanning column, which allows changing the direction of sounding within the upper hemisphere at a speed of 1 degree per second. The polarization characteristics of the transmitter (linear or circular polarization) can be changed by rotating the phase plates synchronously with the laser pulses. Conical scanning of the lidar allows identifying cloud areas with preferential zenith or azimuthal orientation of the crystal particles. The article presents the results of observations of the cloud polarization structure carried out in Tomsk. Methods of the calibrations of lidar polarization channels are described