CAPACITAÇÃO DE EQUIPES PARA INQUÉRITO SOROLÓGICO CANINO PARA PESQUISA DE LEISHMANIOSE VISCERAL

A Leishmaniose Visceral (LV) é uma enfermidade grave, e quando não tratada pode ocasionar letalidade de 95% nos humanos. Em cães, há uma prevalência significativa, com muito dos casos assintomáticos. Diante de tal importância, o diagnóstico precoce em humanos e cães se faz necessário para mapeamento e controle da enfermidade nos municípios. No estado de São Paulo, o primeiro caso desta enfermidade, foi notificado em Araçatuba, em 1998. Desde então, têm ocorrido o avanço da doença no estado principalmente no Noroeste Paulista. Perante tal preocupação, a Prefeitura do município de São José do Rio Preto realizou um inquérito sorológico canino para pesquisa de LV no primeiro semestre de 2017. Para composição das equipes, o inquérito obteve a participação dos alunos do curso de medicina veterinária do Centro Universitário de Rio Preto (UNIRP). Com a finalidade de realizar este inquérito sorológico assegurando o bem-estar animal, a qualidade da amostra para fins em diagnóstico e a educação em saúde foi realizado o treinamento das equipes. Logo, com este trabalho objetiva-se descrever a metodologia de capacitação das equipes para tal inquérito. A capacitação foi realizada por meio de orientação coletiva para descrição e explanação teórica, e supervisão individualizada na ação prática utilizando manequins de cães. O aprendizado foi aplicado por vinte alunos, de abril a maio de 2017, em tutores e em seus cães, residentes na área urbana do Município. Foram coletadas amostras de sangue de 1.581 amostras de sangue de cães. Não foram registradas inconformidades que oferecessem risco ao bem estar animal e ou humano durante a ação. O treinamento otimizou o tempo para coleta de amostras de sangue de cães em área urbana como estratégia de saúde pública

Dengue-4 false negative results by Panbio (R) Dengue Early ELISA assay in Brazil

Background: Dengue is a serious public health problem in numerous countries. the ability to rapidly diagnosis dengue is important for patient triage and management. Detection of dengue viral protein, NS1, represents a new approach to dengue diagnosis.Objective: the present study aims to evaluate if there are false negative results using the NS1 Ag rapid assay (Panbio (R) Dengue Early ELISA) in two different epidemiological situations (epidemic and non-epidemic).Study design: 220 serum samples from patients with clinical symptoms of classical dengue fever were tested by NS1 antigen capture ELISA and Multiplex-Nested-PCR.Results: in samples collected in a non-epidemic period we found a 100% agreement of ELISA and RTPCR in dengue negative samples and 85% agreement of ELISA and RT-PCR in dengue positive samples. But when we tested samples during an epidemic period (large DENV-4 outbreak) we found 15% false negative results (p<0.05) in dengue negative samples.Conclusions: Due to false negative results for DENV-4, the sole use of the Panbio Dengue Early ELISA assay as a screening method for monitoring circulating dengue serotypes must be reevaluated. (C) 2013 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fac Med Sao Jose do Rio Preto FAMERP, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilUniv Estadual Paulista Julio de Mesquitta & Filho, Sao Jose Do Rio Preto, SP, BrazilUniversidade Federal de São Paulo UNIFESP, São Paulo, BrazilPrefeitura Sao Jose do Rio Preto, Dept Vigilancia Saude, Sao Jose Do Rio Preto, SP, BrazilUniv Fed Mato Grosso, Sinop, Mato Grosso, BrazilTufts Univ, Cummings Sch Vet Med, Dept Infect Dis & Global Hlth, North Grafton, MA USAUniversidade Federal de São Paulo UNIFESP, São Paulo, BrazilFAPESP: 2012/11733-6FAPESP: 2011/10458-9Web of Scienc

Adverse birth outcomes associated with Zika virus exposure during pregnancy in São José do Rio Preto, Brazil

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-11-17T12:41:59Z No. of bitstreams: 1 Nogueira ML (PREPRINT) Adverse birth outcomes associated with Zika virus exposure during pregnancy in São....pdf: 2093267 bytes, checksum: 6543c2d2fad9c94caa4b20a635a1eab9 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-11-17T12:43:36Z (GMT) No. of bitstreams: 1 Nogueira ML (PREPRINT) Adverse birth outcomes associated with Zika virus exposure during pregnancy in São....pdf: 2093267 bytes, checksum: 6543c2d2fad9c94caa4b20a635a1eab9 (MD5)Made available in DSpace on 2017-11-17T12:43:36Z (GMT). No. of bitstreams: 1 Nogueira ML (PREPRINT) Adverse birth outcomes associated with Zika virus exposure during pregnancy in São....pdf: 2093267 bytes, checksum: 6543c2d2fad9c94caa4b20a635a1eab9 (MD5) Previous issue date: 2017The São Paulo Research Foundation (FAPESP) via Grant 262 No. 2013/21719-3 and 2016/15021-1for M.L.N, Grant No. 2015/12295-0 for A.C.B.T., and Grant 263 No. 2016/05115-9 for L.C.M. P.F.C.V. was supported by the Zika Virus Fast Track program pro264 vided by the Association for the Improvement of Higher Education Personnel (CAPES) and the 265 Brazilian National Council for Scientific and Technological Development (CNPq) via Grant Nos. 266 303999/2016-0, 440405/2016-5, and 457664/2013-4. MLM is a CNPq Research Fellow.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São Paulo State University. São José do Rio Preto, SP, Brazil.São Paulo State University. São José do Rio Preto, SP, Brazil.São Paulo State University. São José do Rio Preto, SP, Brazil.Evandro Chagas Institute. Ananindeua, PA, Brazil.Evandro Chagas Institute. Ananindeua, PA, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Health Secretariat. São José do Rio Preto, SP, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brazil / Yale School of Public Health. New Haven, Connecticut, USA.Fundacao Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.University of Texas Medical Branch. Galveston, Texas, USA.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.São José do Rio Preto School of Medicine. São José do Rio Preto, SP, Brazil.Yale School of Public Health. New Haven, Connecticut, USA.Objective: We aimed to report the first 54 cases of pregnant women infected by Zika vírus (ZIKV) and their virological and clinical outcomes, as well as the newborns’ outcomes in 2016, after the emergence of ZIKV in dengue endemic areas of São Paulo, Brazil. Methods: This is a descriptive study performed from February to October 2016 on 54 qPCR ZIKV50 positive pregnant women identified by the Public Health Authority of São Jose do Rio Preto, São Paulo, Brazil. The women were followed and had clinical and epidemiological data collected before and after birth. Adverse outcomes in newborns were analyzed and reported. Urine or blood samples from newborns were collected to identify ZIKV infection by RT-PCR. Results: 216 acute Zika-suspected pregnant women were identified, and 54 had the diagnosis con55 firmed by RT-PCR. None of the 54 women miscarried. Among the 54 newborns, 15 exhibited ad56 verse outcomes at birth. The highest number of ZIKV infections occurred during the second and third trimesters. No cases of microcephaly were reported, though the broad clinical spectrum of outcomes, as lenticulostriate vasculopathy, subependymal cysts, auditive and ophtalmological dis59 orders, were identified. ZIKV RNA was detected in 18 of 51 newborns tested and in eight of 15 newborns with adverse outcomes. Conclusions: Although other studies have associated many newborn outcomes to ZIKV infection during pregnancy, these same adverse outcomes were rare or non-existent in this study. The clinical presentation in our newborns was mild compared to other reports, suggesting that there is significant heterogeneity of Congenital Zika Infection