Simultaneous pyometra and viable puppies’ gestation in a bitch

Here we describe a case of pyometra coexisting with gestation in a 4.5 year-old miniature short-haired Dachshund. The dog exhibited depression, vaginal discharge, polydipsia and dehydration. Ultrasound examination revealed the presence of low to moderate anechoic fluid collection in the left uterine horn. Blood analysis revealed mild neutrophilia with a left shift. Based on these findings a presumptive diagnosis of pyometra was made and the bitch was treated using amoxicillin-clavulanate with dopaminergic agonist (cabergoline). A second ultrasound scan revealed the presence of two gestational vesicles in the right uterine horn that were successfully carried to term. Unusually, while pyometra persisted in the left uterine horn, two viable puppies were delivered by caesarean section from the right uterine horn.Fil: Risso, Analia Lorena. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Hospital Escuela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pellegrino, Francisco Javier. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Hospital Escuela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Corrada, Yanina Alejandra. Universidad Nacional de la Plata. Facultad de Cs.veterinarias. Hospital Escuela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Estandarización, evaluación y mejora del entrenamiento deportivo de caninos en cinta trotadora motorizada mediante la administración de ácidos grasos omega 3 preservando la calidad espermática

La popularidad de las competencias deportivas en caninos ha crecido notablemente. Surge así la necesidad de esclarecer parámetros fisiológicos que permitan evaluar con precisión la condición física. Asimismo, el uso de suplementos nutricionales ha despertado interés para mejorar el rendimiento físico. Aunque el principal objetivo es el éxito deportivo, es importante conservar la salud reproductiva. Por lo expuesto, los objetivos generales de esta Tesis fueron: evaluar el efecto de la suplementación con aceite de pescado en caninos durante un programa de entrenamiento, preservando la salud reproductiva; evaluar el uso de pruebas de ejercicios en cinta trotadora y a campo. En un diseño cruzado, ocho perros fueron asignados a dos grupos, control (CNT) y aceite de pescado (AP). Los animales realizaron un programa de entrenamiento en cinta trotadora durante 12 semanas donde se evaluaron variaciones en la frecuencia cardíaca (FC), temperatura rectal (TR) y circunferencia de muslos (CM). Asimismo, cada 4 semanas se realizó a cada animal una prueba de ejercicio en cinta trotadora, evaluación de parámetros espermáticos y concentración testosterona sérica. Para la prueba de ejercicio a campo fueron incluidos 9 perros. Durante el transcurso del entrenamiento, el grupo AP tuvo menor FC y TR y mayor CM que el grupo CNT (P<0,01), presentó mejoras en parámetros espermáticos y menor concentración de testosterona (P<0,01). Además, durante la prueba de ejercicio en cinta trotadora el grupo AP tuvo menor FC y concentración de lactato sanguíneo (LS) (P<0,01). En la prueba de ejercicio a campo, la FC, TR y LS incrementaron significativamente finalizada la misma (P<0,01). El programa de entrenamiento produjo mejoras en el rendimiento físico, sin afectar la salud reproductiva. El uso de pruebas de ejercicio permite valorar la respuesta fisiológica al esfuerzo, siendo los parámetros evaluados precisos para ello. La suplementación con aceite de pescado en caninos puede resultar un complemento nutricional beneficioso para mejorar la respuesta al esfuerzo y el rendimiento en entrenamiento, como también parámetros espermáticos.Tesis co-dirigida por los Dres. Yanina Alejandra Corrada y Daniel Osvaldo Arias.Facultad de Ciencias Veterinaria

Simultaneous pyometra and viable puppies’ gestation in a bitch

Here we describe a case of pyometra coexisting with gestation in a 4.5 year-old miniature short-haired Dachshund. The dog exhibited depression, vaginal discharge, polydipsia and dehydration. Ultrasound examination revealed the presence of low to moderate anechoic fluid collection in the left uterine horn. Blood analysis revealed mild neutrophilia with a left shift. Based on these findings a presumptive diagnosis of pyometra was made and the bitch was treated using amoxicillin-clavulanate with dopaminergic agonist (cabergoline). A second ultrasound scan revealed the presence of two gestational vesicles in the right uterine horn that were successfully carried to term. Unusually, while pyometra persisted in the left uterine horn, two viable puppies were delivered by caesarean section from the right uterine horn.Facultad de Ciencias Veterinaria

Biofísica del aparato locomotor animal

En el organismo animal podemos encontrar los tres estados de agregación de la materia: gaseoso, en el aire que fluye por los alvéolos pulmonares; líquido, en la sangre que circula por los vasos sanguíneos; o sólido, presente en los huesos y músculos que constituyen el sistema músculo esquelético que da sostén, estabilidad y movimiento a los animales.Fil: Pellegrino, Francisco Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Bruzzo Lafratto, Joaquín. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentin

Physiological parameter values in greyhounds before and after high-intensity exercise

Dog sports competitions have greatly expanded. The availability of reference values for each type of activity could help assess fitness accurately. Heart rate (HR), blood lactate (BL) and rectal temperature (RT) are relevant physiological parameters to determine the dogs response to effort. Previous studies in greyhounds have reported the effect of high-intensity exercise on many physiological parameters immediately after completing different racing distances and recovery times. However, there are no studies concerning physiological changes over shorter racing distances. We therefore assessed the effect of sprint exercise on HR, BL and RT in nine greyhounds performing sprint exercise over a 100-m distance chasing a lure. After the exercise, dogs underwent a passive 10-min recovery phase. Before the exercise, immediately after it and at 5 and 10 min during recovery, HR and RT were assessed and blood samples were collected for BL determination. HR, BL and RT values increased significantly after the exercise (P0.1), BL concentration and RT remained increased (P<0.01). The abrupt increase in HR, BL and RT values observed immediatel y after the exercise indicates the high intensity of the effort performed. Similarly, BL concentration after the exercise exceeded the 4 mmol/L lactate threshold, suggesting a predominant anaerobic metabolism during effort. Although HR returned to pre-exercise values 10 min after the exercise, a more extensive recovery phase would be necessary for a total return to resting values, particularly for BL and RT. In greyhounds subjected to high-intensity exercise, HR, BL and RT were reliable physiological parameters to accurately assess the physiological response to effort. The use of sprint exercises over short racing distances could be useful for appropriately monitoring fitness in sporting dogs.Instituto de Genética Veterinari

Physiological parameter values in greyhounds before and after high-intensity exercise

Dog sports competitions have greatly expanded. The availability of reference values for each type of activity could help assess fitness accurately. Heart rate (HR), blood lactate (BL) and rectal temperature (RT) are relevant physiological parameters to determine the dogs response to effort. Previous studies in greyhounds have reported the effect of high-intensity exercise on many physiological parameters immediately after completing different racing distances and recovery times. However, there are no studies concerning physiological changes over shorter racing distances. We therefore assessed the effect of sprint exercise on HR, BL and RT in nine greyhounds performing sprint exercise over a 100-m distance chasing a lure. After the exercise, dogs underwent a passive 10-min recovery phase. Before the exercise, immediately after it and at 5 and 10 min during recovery, HR and RT were assessed and blood samples were collected for BL determination. HR, BL and RT values increased significantly after the exercise (P0.1), BL concentration and RT remained increased (P<0.01). The abrupt increase in HR, BL and RT values observed immediatel y after the exercise indicates the high intensity of the effort performed. Similarly, BL concentration after the exercise exceeded the 4 mmol/L lactate threshold, suggesting a predominant anaerobic metabolism during effort. Although HR returned to pre-exercise values 10 min after the exercise, a more extensive recovery phase would be necessary for a total return to resting values, particularly for BL and RT. In greyhounds subjected to high-intensity exercise, HR, BL and RT were reliable physiological parameters to accurately assess the physiological response to effort. The use of sprint exercises over short racing distances could be useful for appropriately monitoring fitness in sporting dogs.Instituto de Genética Veterinari

Evaluation of total antioxidant activity and oxidative stress in seminal plasma from dogs supplemented with fish oil and vitamin E

Background: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs. CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0% vs. CG and+60.2% vs. FOEG) compared with the other two groups (P<0.05). Serum triglyceride (TG) concentration decreased in FOG and FOEG compared with CG, on day 60 (P<0.01). Conclusion: We concluded that total antioxidant activitydecreased and oxidative stress increased in seminal plasma of dogs after FO supplementation for 60 days.Fil: Risso, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Pellegrino, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Schinella, Guillermo Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Universidad Nacional Arturo Jauretche; Argentina. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentin

Effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program

El objetivo del presente estudio fue evaluar el efecto de la suplementación con aceite de pescado sobre la frecuencia cardíaca y temperatura rectal en perros sanos durante un programa de entrenamiento en cinta trotadora. En un diseño experimental cruzado, 5 perros machos fueron asignados aleatoriamente a los grupos control y aceite de pescado, y recibieron diariamente 54 mg de aceite de pescado/kg de peso corporal0,75. Todos fueron entrenados en cinta trotadora dos veces por semana durante 12 semanas. Cada sesión incluyó un total de 30 minutos a 8 km/h y 7,5 % de pendiente. Se evaluó la frecuencia cardíaca previa, inmediatamente posterior y a los 5 minutos de finalizada cada sesión de entrenamiento. Se midió la temperatura rectal previa y posterior a cada sesión. En el grupo aceite de pescado, la frecuencia cardíaca y temperatura rectal posteriores a cada sesión de entrenamiento disminuyeron significativamente (P0,1). Los resultados obtenidos podrían reflejar una mejora en el rendimiento en perros suplementados con aceite de pescado durante el entrenamiento en cinta trotadora. Sin embargo, sería interesante realizar más estudios que permitan explicar los mecanismos por los cuales la suplementación con aceite de pescado podría mejorar el rendimiento en entrenamiento.The aim of this study was to assess the effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program. In a crossover experimental design, 5 male dogs were randomly assigned to control and fish oil groups, and received 54 mg fish oil/kg of body weight0.75 per day. All dogs were trained on a treadmill twice a week for 12 weeks. Each session was 30 minutes long, at 8 km/h speed and a 7.5 % slope. Heart rate was assessed before, immediately after and 5 minutes after finishing each training session. Rectal temperature was measured before and after each session. In the fish oil group, heart rate and rectal temperature after each training session were significantly reduced (P0.1). The results obtained might show an improvement in the performance of dogs receiving fish oil supplementation during treadmill training. However, it would be interesting to conduct further studies to explain the mechanisms by which fish oil supplementation might improve performance during training.Facultad de Ciencias Veterinaria

Effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program

El objetivo del presente estudio fue evaluar el efecto de la suplementación con aceite de pescado sobre la frecuencia cardíaca y temperatura rectal en perros sanos durante un programa de entrenamiento en cinta trotadora. En un diseño experimental cruzado, 5 perros machos fueron asignados aleatoriamente a los grupos control y aceite de pescado, y recibieron diariamente 54 mg de aceite de pescado/kg de peso corporal0,75. Todos fueron entrenados en cinta trotadora dos veces por semana durante 12 semanas. Cada sesión incluyó un total de 30 minutos a 8 km/h y 7,5 % de pendiente. Se evaluó la frecuencia cardíaca previa, inmediatamente posterior y a los 5 minutos de finalizada cada sesión de entrenamiento. Se midió la temperatura rectal previa y posterior a cada sesión. En el grupo aceite de pescado, la frecuencia cardíaca y temperatura rectal posteriores a cada sesión de entrenamiento disminuyeron significativamente (P0,1). Los resultados obtenidos podrían reflejar una mejora en el rendimiento en perros suplementados con aceite de pescado durante el entrenamiento en cinta trotadora. Sin embargo, sería interesante realizar más estudios que permitan explicar los mecanismos por los cuales la suplementación con aceite de pescado podría mejorar el rendimiento en entrenamiento.The aim of this study was to assess the effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program. In a crossover experimental design, 5 male dogs were randomly assigned to control and fish oil groups, and received 54 mg fish oil/kg of body weight0.75 per day. All dogs were trained on a treadmill twice a week for 12 weeks. Each session was 30 minutes long, at 8 km/h speed and a 7.5 % slope. Heart rate was assessed before, immediately after and 5 minutes after finishing each training session. Rectal temperature was measured before and after each session. In the fish oil group, heart rate and rectal temperature after each training session were significantly reduced (P0.1). The results obtained might show an improvement in the performance of dogs receiving fish oil supplementation during treadmill training. However, it would be interesting to conduct further studies to explain the mechanisms by which fish oil supplementation might improve performance during training.Facultad de Ciencias Veterinaria

Effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program

El objetivo del presente estudio fue evaluar el efecto de la suplementación con aceite de pescado sobre la frecuencia cardíaca y temperatura rectal en perros sanos durante un programa de entrenamiento en cinta trotadora. En un diseño experimental cruzado, 5 perros machos fueron asignados aleatoriamente a los grupos control y aceite de pescado, y recibieron diariamente 54 mg de aceite de pescado/kg de peso corporal0,75. Todos fueron entrenados en cinta trotadora dos veces por semana durante 12 semanas. Cada sesión incluyó un total de 30 minutos a 8 km/h y 7,5 % de pendiente. Se evaluó la frecuencia cardíaca previa, inmediatamente posterior y a los 5 minutos de finalizada cada sesión de entrenamiento. Se midió la temperatura rectal previa y posterior a cada sesión. En el grupo aceite de pescado, la frecuencia cardíaca y temperatura rectal posteriores a cada sesión de entrenamiento disminuyeron significativamente (P0,1). Los resultados obtenidos podrían reflejar una mejora en el rendimiento en perros suplementados con aceite de pescado durante el entrenamiento en cinta trotadora. Sin embargo, sería interesante realizar más estudios que permitan explicar los mecanismos por los cuales la suplementación con aceite de pescado podría mejorar el rendimiento en entrenamiento.The aim of this study was to assess the effect of fish oil supplementation on heart rate and rectal temperature in healthy dogs during a treadmill training program. In a crossover experimental design, 5 male dogs were randomly assigned to control and fish oil groups, and received 54 mg fish oil/kg of body weight0.75 per day. All dogs were trained on a treadmill twice a week for 12 weeks. Each session was 30 minutes long, at 8 km/h speed and a 7.5 % slope. Heart rate was assessed before, immediately after and 5 minutes after finishing each training session. Rectal temperature was measured before and after each session. In the fish oil group, heart rate and rectal temperature after each training session were significantly reduced (P0.1). The results obtained might show an improvement in the performance of dogs receiving fish oil supplementation during treadmill training. However, it would be interesting to conduct further studies to explain the mechanisms by which fish oil supplementation might improve performance during training.Facultad de Ciencias Veterinaria