The correlation between immunohistochemical expression of MMP-2 and the prognosis of epithelial ovarian cancer

Objectives: The Objectives: The goal of the study was to evaluate the correlation of matrix metalloproteinase-2 (MMP-2) expression with tumor spread, metastasis, survival and recurrence in early and advanced-stage Epithelial Ovarian Cancer (EOC). Material and methods: Medical records of patients, hospitalized at the Department of Obstetrics and Gynecology, İzmir Ataturk Training and Research Hospital between 2003 and 2008, were reviewed. Patient age, tumor size, localization, histologic type and tumor grade, stage, metastasis status, patient outcomes and follow-up data were obtained from the records of the obstetrics and gynecology clinic, as well as during face-to-face or telephone interviews. Results: The percentage of MMP-2 staining (expression) in the epithelial cells was not significantly associated with tumor stage and grade, histologic type, tumor diameter, recurrence and overall survival (p>0.05). A significant correlation was found between the percentage of MMP-2 staining (expression) and metastasis status (p0.05), but was with histologic type (p0,05). Stromal staining (expression) of MMP-2 was not significantly correlated with tumor stage and grade, histologic type, tumor diameter and outcomes (p>0.05), but was with recurrence and presence of metastasis (p0.05), total score (p>0.05) and staining intensity (p>0.05). The association of disease-free survival with the percentage of MMP-2 staining (p>0.05), total score (p>0.05), staining intensity (p>0.05) and stromal staining (p>0.05) was not statistically significant. The survival of patients with positive stromal staining was significantly shorter compared to cases with negative stromal staining (

Microinfiltracao de ionomero de vidro de alta viscosidade e carbômero de vidro com e sem revestimento antes e depois de envelhecimento hidrotermal

Objective: The objective of this study was to evaluate the microleakage patterns of GIC and GGC with and without their protective surface coatings on enamel and dentin margins before and after aging. Material and Methods: Two rectangular cavities (height: 2 mm; width: 3 mm; depth: 1.5 mm) were prepared on each tooth at the cemento-enamel junction were prepared on human permanent molars (N=56) and the teeth were randomly assigned to be restored with one of the following: a) high viscosity glass-ionomer cement (GIC) (EQUIA Fil, C Corp., Tokyo, Japan) (n=28), b) glass-carbomer cement (GCC) (Glass Carbomer Products, Leiden, The Netherlands) (n=28). Half of the teeth were further divided into two groups where one group received protective surface coating (SC) (G-Coat Plus, GC Corp) (n=14) and the other group did not (n=14). Half of the teeth were stored for 24 hours (n=7), and the other half was thermocycled (5000 cycles, 5-55°C) (n=7). For microleakage analysis, the teeth were immersed in 5% methylene blue dye for 24 hours, sectioned into two equal halves. Microleakage patterns were evaluated using stereomicroscope and scored on a scale of 0-3 (0: No dye penetration, 1: Dye penetration less than half of the axial wall, 2: Dye penetration more than half the axial wall, 3: Dye penetration spreading along the axial wall). Data were analyzed using Kruskal-Wallis tests at the significance level of 0.05. Results: Compared to 24 h storage, after thermocycling, coating on GIC decreased microleakage significantly compared to GCC (p=0.046) but not for GCC. In the thermocycled groups, coated GIC showed significantly less leakage at the enamel margin but no significant difference was found with both GIC and GCC in the dentin margins. Conclusion: The application of surface coating significantly reduced the microleakage scores of GIC but not GCC, within the enamel margins only.Objetivo: O objetivo deste estudo foi avaliar os padrões de microinfiltração de GIC e GGC com e sem seus revestimentos protetores superficiais nas margens de esmalte e dentina antes e após o envelhecimento. Material e Métodos: duas cavidades retangulares (altura: 2 mm; largura: 3 mm; profundidade: 1,5 mm) foram preparadas em cada dente na junção cemento-esmalte de molares permanentes humanos (N = 56), sendo aleatoriamente designados para serem restaurados com um dos seguintes: a) cimento de ionômero de vidro (GIC) de alta viscosidade (EQUIA Fil, C Corp., Tóquio, Japão) (n = 28), b) cimento de vidrocarbômero (GCC) Carbomer Products, Leiden, Holanda) (n = 28). Metade dos dentes foram divididos em dois grupos, onde um grupo recebeu revestimento protetor de superfície (SC) (G-Coat Plus, GC Corp) (n = 14) e o outro grupo não (n = 14). Metade dos dentes foram armazenados por 24 horas (n = 7), e a outra metade foi termociclada (5000 ciclos, 5-55 ° C) (n = 7). Para análise de microinfiltração, os dentes foram imersos em corante azul de metileno a 5% por 24 horas, seccionados em duas metades iguais. Os padrões de microinfiltração foram avaliados usando estereomicroscópio e pontuados numa escala de 0-3 (0: Sem penetração de corante; 1: penetração de corante inferior à metade da parede axial; 2: penetração de corante mais do que metade da parede axial; 3: penetração de corante ao longo da parede axial). Os dados foram analisados pelo teste de Kruskal-Wallis ao nível de significância de 0,05. Resultados: em comparação com o armazenamento de 24 h, após a termociclagem, o revestimento de superfície no GIC diminuiu significativamente a microinfiltração em comparação com o GCC (p = 0,046), mas não para o GCC. Nos grupos termociclados, o GIC revestido apresentou significativamente menos infiltração na margem do esmalte, mas não houve diferença significativa para o GIC e o GCC nas margens dentinárias. Conclusão: A aplicação do revestimento de superfície reduziu significativamente os escores de microinfiltração do GIC, mas não do GCC, apenas nas margens do esmalte

Evaluation of relationship between disease severity, mean platelet volume, and platelet distribution width in stable chronic obstructive pulmonary disease

Aim: This study was conducted to evaluate the relationship between disease severity, mean platelet volume and platelet distribution width in stable chronic obstructive pulmonary disease. Methods: The study included 120 consecutive patients with stable chronic obstructive pulmonary disease and 30 consecutive age-matched healthy subjects (control group). Patients were classified as A (mild), B (mild to moderate), C (moderate to severe) and D (severe) defined by the GOLD committee and grouped as A/B (n= 60) and C/D (n=60).Results: Platelet levels were not different among the groups. Mean platelet volume was lower in all patients than control group (p=0.001). Level of platelet distribution width was higher in all patients than control group (p=0.018). Mean platelet volume in C/D groups were significantly lower than A/B group (p=0.011) and control group (p=0.001). Mean platelet volume in A/B group were also significantly lower than control group (p=0.001). Erythrocyte sedimentation rates were higher in A/B and C/D groups than control group (p=0.007 and p=0.001, respectively). C-reactive protein levels in control group were significantly lower than C/D group (p=0.001). No statistically significant correlations were observed between mean platelet volume and forced expiratory volume in one second and forced expiratory volume in one second/forced vital capacity or between mean platelet volume and other inflammatory parameters in A/B or C/D groups. Significant positive correlations were found between erythrocyte sedimentation rate and C-reactive protein (r=0.375; p=0.003), and between mean platelet volume and platelet large cell ratio (r=0.749; p=0.001) in C and D groups. Conclusion: It was concluded that mean platelet volume could be used as a negative acute‑phase reactant in evaluation of disease severity of chronic obstructive pulmonary disease as C-reactive protein

Determinaation of DNA damage caused by IR rays by electrochemical methods and investigation of the damage in the presence of some molecules

Amaç: Bu çalışma, IR ışınlarının neden olduğu DNA hasarının, Guanin bazının yükseltgenme sinyalinde meydana gelen değişimin elektrokimyasal olarak ölçülerek tespit edilmesi ve söz konusu hasarın belirli vitaminlerin ve polimerlerin varlığında nasıl değiştiğinin incelenmesini amaçlamaktadır. Materyal ve Yöntem: Tek kullanımlık kalem grafit elektrot yüzeyine immobilize edilen çift sarmal DNA belirli süreler IR ışığına maruz bırakıldı. Maruziyetten önce ve sonra Guanin yükseltgenme sinyalindeki değişim incelendi. Ayrıca elektrot yüzeyine immobilize edilmiş olan DNA'nın üzerine vitamin/doğal polimer kaplanarak söz konusu hasarın değişimi incelenmiştir. Böylece söz konusu vitamin/doğal polimerin hasarı engellemedeki etkisi araştırılmıştır. Bulgular ve Sonuç: Çalışmada, C, B7, D ve E vitaminleri ile doğal polimerler olan kitosan ve polietilen glikol (PEG)'in IR ışınlarından kaynaklanan hasarı önlemeye etkisi araştırılmış ve C ve E vitaminlerinin hasarı engellemde etkili olmadığı, D ve B7 vitaminleri ile kitosan ve PEG polimerlerinin, DNA molekülünün IR ışınlarının etkisiyle hasara uğramasını engellediği tespit edilmiştir.Objective: This study aims to detect DNA damage caused by IR rays by measuring the change in the oxidation signals of Guanine electrochemically and to examine the changes in this damage in the presence of certain vitamins and polimers. Materials and Methods: Double-stranded DNA was immobilized onto the surface of the disposable pencil graphite electrode and was exposed to IR light for certain periods. The change in the oxidation signal of guanine before and after exposure was measured. In addition, the DNA immobilized electrode surface was coated with vitamin/natural polymer and exposed to IR rays, and the guanine oxidation signal was measured before and after exposure. In this way, it was investigated the effect of vitamin/ polymers on preventing DNA damage caused by IR rays. Results and Conclusion: In this study, the effects of Vitamin C, B7, D and E and natural polymers, chitosan and polyethylene glycol (PEG), on the preventing of DNA damage caused by IR rays were investigated. It has been determined that vitamins C and E are not effective in preventing damage, and vitamins D and B7, as well as chitosan and PEG polymers, prevent the DNA molecule from being damaged by the effect of IR rays

Relationship between Circulating Serpina3g, Matrix Metalloproteinase-9, and Tissue Inhibitor of Metalloproteinase-1 and -2 with Chronic Obstructive Pulmonary Disease Severity

Chronic obstructive pulmonary disease (COPD) is influenced by genetic and environmental factors. A protease-antiprotease imbalance has been suggested as a possible pathogenic mechanism for COPD. Here, we examined the relationship between circulating serpina3g, matrix metalloproteinase-9 (MMP-9), and tissue inhibitor of metalloproteinase-1 and -2 (TIMP-1 and -2, respectively) and severity of COPD. We included 150 stable COPD patients and 35 control subjects in the study. The COPD patients were classified into four groups (I, II, III, and IV), according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) guidelines based on the severity of symptoms and the exacerbation risk. Plasma serpina3g, MMP-9, and TIMP-1 and -2 concentrations were significantly higher in the all patients than in control subjects. Plasma serpina3g, MMP-9, and TIMP-1 and -2 concentrations were significantly higher in groups III and IV than in groups I and II. A negative correlation between serpina3g, MMP-9, and TIMP-1 and -2 levels and the forced expiratory volume in 1 s (FEV1) was observed. MMP-9 concentration and the MMP-9/TIMP-1 ratio were higher in patients with emphysema than in other phenotypes (both with p < 0.01). The findings of this study suggest that circulating serpina3g, MMP-9, and TIMP-1 and -2 levels may play an important role in airway remodeling in COPD pathogenesis. Disrupted protease-antiprotease imbalance in patients with COPD is related to the presence of airway injury. MMP-9 concentration and the MMP-9/TIMP-1 ratio are the best predictors of emphysema in COPD patients

Microleakage of high viscosity glass-ionomer and glass-carbomer with and without coating before and after hydrothermal aging

Objective: The objective of this study was to evaluate the microleakage patterns of GIC and GGC with and without their protective surface coatings on enamel and dentin margins before and after aging. Material and Methods: Two rectangular cavities (height: 2 mm; width: 3 mm; depth: 1.5 mm) were prepared on each tooth at the cemento-enamel junction were prepared on human permanent molars (N=56) and the teeth were randomly assigned to be restored with one of the following: a) high viscosity glass-ionomer cement (GIC) (EQUIA Fil, C Corp., Tokyo, Japan) (n=28), b) glass-carbomer cement (GCC) (Glass Carbomer Products, Leiden, The Netherlands) (n=28). Half of the teeth were further divided into two groups where one group received protective surface coating (SC) (G-Coat Plus, GC Corp) (n=14) and the other group did not (n=14). Half of the teeth were stored for 24 hours (n=7), and the other half was thermocycled (5000 cycles, 5-55°C) (n=7). For microleakage analysis, the teeth were immersed in 5% methylene blue dye for 24 hours, sectioned into two equal halves. Microleakage patterns were evaluated using stereomicroscope and scored on a scale of 0-3 (0: No dye penetration, 1: Dye penetration less than half of the axial wall, 2: Dye penetration more than half the axial wall, 3: Dye penetration spreading along the axial wall). Data were analyzed using Kruskal-Wallis tests at the significance level of 0.05. Results: Compared to 24 h storage, after thermocycling, surface coating on GIC decreased microleakage significantly compared to GCC (p=0.046) but not for GCC. In the thermocycled groups, coated GIC showed significantly less leakage at the enamel margin but no significant difference was found with both GIC and GCC in the dentin margins. Conclusion: The application of surface coating significantly reduced the microleakage scores of GIC but not GCC, within the enamel margins only.KeywordsGlass-carbomer; Glass-ionomer; Microleakage

Bilateral non-arteritic anterior ischemic optic neuropathy following second-trimester spontaneous abortion-related haemorrhage

WOS: 000309640500026PubMed: 22898194Bilateral anterior ischemic optic neuropathy is a rare complication of massive haemorrhage and related hypotension and anaemia in young individuals. We report a 34-year-old woman with bilateral non-arteritic ischemic optic neuropathy (NAION) after a massive spontaneous abortion-related haemorrhage who presented with sudden painless visual loss in her left eye. Visual acuity was 20/20 in the right eye with only hand motion discernible in the left eye. There was a left relative afferent papillary defect (RAPD). Fundus examination revealed bilateral swollen, hyperaemic optic discs and nerve fiber layer haemorrhages. Brain MRI and magnetic resonance venography were normal. The diagnosis of bilateral NAION was made and intravenous pulse corticosteroid therapy (1000 mg/day) was administered for three days. On the sixth clay, optic disc oedema regressed bilaterally and on the third week, the visual acuity improved to 20/80 in the left eye. The visual field showed only a small spared area in the nasal region, and persistent RAPD was present. After two months, fundus examination showed a small and crowded optic disc on the right and a pale optic disc on the left. Severe acute haemorrhage is an important risk factor for NAION in healthy young individuals. In addition to correction of hypotension and anaemia, intravenous high dose corticosteroid might be beneficial for treatment. (C) 2012 Elsevier Ltd. All rights reserved

IMMUNE MARKERS IN SURGICALLY RESECTED EARLY STAGE NON-SMALL CELL LUNG CANCER PATIENTS

Purpose: Non-small cell lung cancer (NSCLC) is the most common cancer and the leading cause of cancer-related deaths worldwide. Inflammatory markers, such as C-reactive protein (CRP), neutrophil-lymphocyte ratio (NLR), lymphocyte-monocyte ratio and platelet-lymphocyte ratio (PLR), serve as indexes for the immune status of the host and the degree of tumor progression. In this study, we evaluated NLR and PLR values in early stage lung cancer patients before and after surgery and its correlation with clinical variables and survival

Perception of Symmetry in Aesthetic Rhinoplasty Patients: Anthropometric, Demographic, and Psychological Analysis

WOS: 000411436000003PubMed ID: 28614976Visual perception of symmetry is a major determinant of satisfaction after aesthetic rhinoplasty. In this study, we sought to investigate the existence of any relationship between anthropometric characteristics of the face and visual perceptions of asymmetry among rhinoplasty patients and to evaluate tools that can shed light on patients who appear at high risk for exaggerating potential asymmetries. In the first part, 168 rhinoplasty patients were asked to fill out the demographic questionnaire, nasal shape evaluation scale, and the somatosensory amplification scale. In the second part, we examined the relationship between anthropometric characteristics of the face and visual perceptions of asymmetry using standardized photographs of 100 medical students. In the third part, patients answered the rhinoplasty outcome evaluation questionnaire 6 months after the surgery. Objectively, no symmetrical face was observed in the anthropometric evaluation. Subjectively, only 73% and 54% of the faces were considered asymmetrical by the rhinoplasty and the control groups, respectively. The rate of asymmetry perception was significantly greater in revision patients when compared with primary rhinoplasty patients. The relationship between the rate of subjective perception of asymmetry and the somatosensory amplification scale scores was statistically significant. We found a significant inverse relationship between the rate of asymmetry perception and the rhinoplasty outcome evaluation scores. Plastic surgeons should be aware of this high selectivity in asymmetry perception, which is associated with poor postoperative satisfaction. Somatosensory amplification scale may help identify rhinoplasty patients at a high risk for exaggerating potential asymmetries