5 research outputs found
Gene network downstream plant stress response modulated by peroxisomal H2O2
Reactive oxygen species (ROS) act as secondary messengers that can be sensed
by specific redox-sensitive proteins responsible for the activation of signal
transduction culminating in altered gene expression. The subcellular site, in
which modifications in the ROS/oxidation state occur, can also act as a specific
cellular redox network signal. The chemical identity of ROS and their subcellular
origin is actually a specific imprint on the transcriptome response. In recent
years, a number of transcriptomic studies related to altered ROS metabolism in
plant peroxisomes have been carried out. In this study, we conducted a metaanalysis
of these transcriptomic findings to identify common transcriptional
footprints for plant peroxisomal-dependent signaling at early and later time
points. These footprints highlight the regulation of various metabolic pathways
and gene families, which are also found in plant responses to several abiotic
stresses. Major peroxisomal-dependent genes are associated with protein
and endoplasmic reticulum (ER) protection at later stages of stress while, at
earlier stages, these genes are related to hormone biosynthesis and signaling
regulation. Furthermore, in silico analyses allowed us to assign human orthologs
to some of the peroxisomal-dependent proteins, which are mainly associated
with different cancer pathologies. Peroxisomal footprints provide a valuable
resource for assessing and supporting key peroxisomal functions in cellular
metabolism under control and stress conditions across species.Spanish Ministry of Science, Innovation and Universities (MCIU)State Research Agency (AEI)FEDER grant PGC2018-098372-B-I00MCIU Research Personnel Training (FPI) grant BES-2016-07651
Función de la peroxina PEX11a en la interacción planta-patógeno
Comunicación de congreso presentada en: II Jornada de la Juventud Investigadora. Granada, España. 18 octubre (2023
Nitric Oxide and Globin Glb1 Regulate Fusarium oxysporum Infection of Arabidopsis thaliana
Plants continuously interact with fungi, some of which, such as Fusarium oxysporum, are lethal, leading to reduced crop yields. Recently, nitric oxide (NO) has been found to play a regulatory role in plant responses to F. oxysporum, although the underlying mechanisms involved are poorly understood. In this study, we show that Arabidopsis mutants with altered levels of phytoglobin 1 (Glb1) have a higher survival rate than wild type (WT) after infection with F. oxysporum, although all the genotypes analyzed exhibited a similar fungal burden. None of the defense responses that were analyzed in Glb1 lines, such as phenols, iron metabolism, peroxidase activity, or reactive oxygen species (ROS) production, appear to explain their higher survival rates. However, the early induction of the PR genes may be one of the reasons for the observed survival rate of Glb1 lines infected with F. oxysporum. Furthermore, while PR1 expression was induced in Glb1 lines very early on the response to F. oxysporum, this induction was not observed in WT plants
ROS and redox regulation of cell-to-cell and systemic signaling in plants during stress
Stress results in the enhanced accumulation of reactive oxygen species (ROS) in plants, altering the redox state of cells and triggering the activation of multiple defense and acclimation mechanisms. In addition to activating ROS and redox responses in tissues that are directly subjected to stress (termed 'local' tissues), the sensing of stress in plants triggers different systemic signals that travel to other parts of the plant (termed 'systemic' tissues) and activate acclimation and defense mechanisms in them; even before they are subjected to stress. Among the different systemic signals triggered by stress in plants are electric, calcium, ROS, and redox waves that are mobilized in a cell-to-cell fashion from local to systemic tissues over long distances, sometimes at speeds of up to several millimeters per second. Here, we discuss new studies that identified various molecular mechanisms and proteins involved in mediating systemic signals in plants. In addition, we highlight recent studies that are beginning to unravel the mode of integration and hierarchy of the different systemic signals and underline open questions that require further attention. Unraveling the role of ROS and redox in plant stress responses is highly important for the development of climate resilient crops
Peroxisomes: a redox-signaling node in intracellular communication and stress response
Conferencia invitada presentada en: XX Congreso de la Sociedad española de Biología celular. Córdoba, España, 13-15 noviembre (2023