A computational insight into acetylcholinesterase inhibitory activity of a new lichen depsidone

Acetylcholinesterase (AChE) inhibitors are yet the best drugs currently available for the management of Alzheimer's disease. The recent phytochemical investigation has led to the isolation of a new depsidone 1 with moderate AChE activity (1 mu g). This work was focused on its electronic properties analysed using commercially available programs. Both the active depsidone molecule 1 and galanthamine showed to have higher HOMO energies than the inactive depsidones 2-4, isolated from the same lichen species. However, the amino depsidone derivative 7, whose structure was proposed using computational approaches, is expected to be more active AChE inhibitor than the depsidone 1, due to the improved HOMO energy value. In addition, the molecular docking study indicated that the compound 7 has ability to make the well-known interactions of potent AChE inhibitors with the enzyme active site. The data presented herein support the design of novel AChE inhibitors based on the depsidone scaffold

Avarol effectively inhibits bacillus cereus growth

This work extends in vitro screening of antibacterial activity of avarol on four selected pathogenic bacteria associated with psoriasis (Bacillus cereus, Clostridium sporogenes, Enterococcus faecalis and Staphylococcus aureus). Avarol exhibited a moderate activity with minimum inhibitory and minimum bactericidal concentrations ranged from 0.78-1.56 and 3.12-18.75 μg/mL, respectively. It proved to be more effective against B. cereus in comparison to the both positive controls applied, gentamycin and ampicillin. According to the experimental data, this marine natural product may inspire development of new antibiotics with an enhanced therapeutic index, at first place those targeting B. cereus

Ampelografski opis grozda, bobice i semenke sorte Merlo (Vitis vinifera L.) - i odabranih klonova

During a four-year period, ampelographic experiments focusing on the berry cluster (average length of grape cluster, number of grape clusters per shoot, number of berries per grape cluster and length of peduncle), berry (length of berry and berry juice yield), length of pedicel and seed (length of berry seed) of Merlot cultivar (used as a relevant standard) and 11 clones (Nos. 022, 023, 025, 026, 027, 028, 029, 030, 031, 033 and 034) were performed in order to establish the differences among them. These experiments were actually conducted in the third phase of individual clonal selection of Merlot cultivar carried out in Serbia. The lengths of grape cluster and pedicel as well as berry must yields differed significantly among the examined clones. The cluster and principal component analyses classified 12 samples into three divergent clusters/groups, respectively. The clones belonging to the cluster II /the second group/ had significantly higher values of numbers of grape clusters per shoot and berries per grape cluster; lengths of peduncle and berry; berry must yield and length of pedicel, compared both to standard Merlot /the cluster I, the first group/ and the clones of the cluster III /the third group/. The phenological observations showed no significant differences in the beginnings and durations of phenological stages and vegetation period of the examined clones. The obtained results indicate the real need for further research work focused both on the agrobiological and technological properties of the grapes and wines aiming to better describe the selected clones.Tokom četvorogodišnjeg perioda urađen je ampelografski opis grozda, bobice i semenke jedanaest klonova (022, 023, 025, 026, 027, 028, 029, 030, 031, 033 i 034) i sorte Merlo (kao standarda) u cilju utvrđivanja razlike među njima. Datim ispitivanjem (sprovedenim u Srbiji) obuhvaćene su sledeće karakteristike: broj grozdova po izdanku, dužina grozda, broj bobica po grozdu, dužina stabljike grozda, dužina bobice, randman soka bobice, dužina peteljčice bobica i dužina semenke. Eksperimenti su urađeni u toku treće faze individualne klonske selekcije sorte Merlo. Proučavani klonovi značajno su se razlikovali među sobom, a posebno prema dužini stabljike grozda, randmanu soka bobice i dužini peteljčice bobice. Dobijeni rezultati obrađeni su hijerarhijskom klasterskom analizom (HCA) i multivarijantnom analizom podataka (pomoću analize glavnih komponenata - PCA). Shodno tome, dati uzorci (standard i varijeteti) svrstani su u tri klastera (HCA), odnosno grupe (PCA). Klonovi obuhvaćeni klasterom II /druga grupa/ odlikovali su se značajno većim vrednostima broja grozdova po izdanku, broja bobica po grozdu, dužine stabljike grozda, dužine bobice, randmana soka bobice i dužine peteljčice bobice kako u odnosu na Merlo standard, tako i u odnosu na klonove klastera III /treća grupa/. Kao takvi, mogu biti obuhvaćeni daljim proučavanjima u okviru klonske selekcije. Sa fenološkog aspekta, pak, među datim klonovima nisu uočene značajnije razlike u početku i trajanju pojedinačnih razvojnih fenofaza, kao i u dužini vegetacionog perioda

Hemija masnih kiselina Atrichum undulatum i Hypnum andoi

The fatty acid compositions of the moss species Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) and Hypnum andoi A.J.E. Sm. (Hypnaceae) collected in winter time were analysed by gas chromatography (GC) and gas chromatography mass spectrometry (GC-MS) as a contribution to their chemistry. Eight fatty acids were identified in the chloroform/methanol extract 1:1 of A. undulatum (linoleic acid, 26.80%, palmitic acid, 22.17%, alpha-linolenic acid, 20.50%, oleic acid, 18.49%, arachidonic acid, 6.21%, stearic acid, 3.34%, cis-5,8,11,14,17-eicosapentaenoic acid, 1.52% and behenic acid, 1.01%), while six fatty acids were found in the same type of extract of H. andoi (palmitic acid, 63.48%, erucic acid, 12.38%, stearic acid, 8.08%, behenic acid, 6.26%, lignoceric acid, 5.16% and arachidic acid, 4.64%). According to this study, the moss A. undulatum can be considered as a good source of both essential fatty acids for humans (linoleic acid and alpha-linolenic acid) during the winter.Kao doprinos izučavanju njihovog hemijskog sastava, u ovom radu GC i GC-MS analizom ispitivane su masne kiseline mahovina Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) i Hypnum andoi A.J.E. Sm. (Hypnaceae) sakupljene za vreme zimskog doba. U ekstraktu hloroform/metanol 1:1 vrste A. undulatum identifikovano je osam viših masnih kiselina (linolna kiselina, 26,80%, palmitinska kiselina, 22,17%, α-linolenska kiselina, 20,50%, oleinska kiselina, 18,49%, arahidonska kiselina, 6,21%, stearinska kiselina, 3,34%, cis-5,8,11,14,17-eikosapentaenska kiselina, 1,52% i behenska kiselina, 1,01%), dok je u istom ekstraktu vrste H. andoi nađeno šest viših masnih kiselina (palmitinska kiselina, 63,48%, erukinska kiselina, 12,38%, stearinska kiselina, 8,08%, behenska kiselina, 6,26%, lignocerinska kiselina, 5,16% i arahidinska kiselina, 4,64%). Dobijeni rezultati ukazuju da u ispitivanom vremenskom periodu mahovina A. undulatum predstavlja dobar izvor linolne i α-linolenske kiseline, esencijalnih masnih kiselina za ljude

An Insight into Fatty Acid Composition of Calliergonella cuspidata

The fatty acid composition of the moss Calliergonella cuspidata (Hedw.) Loeske (Amblystegiaccae) was preliminary analyzed by gas chromatography (GC FID) and gas chromatography-mass spectrometry (GC-MS). Six fatty acids were identified in its chloroform-methanol extract 1/1: palmitic acid (66.83 %), stearic acid (11.73%), oleic acid (8.21 %), linolenic acid (6.01 %), alpha-linolenic acid (3.95 %) and arachidic acid (3.26 %). Arachidonic acid and cis-5,8,11,14,17-eicosapentaenoic acid, typical long-chain polyunsaturated fatty acids for many mosses, were not detected in Calliergonella cuspidata

Further in vitro biological activity evaluation of amino-, thio- and ester-derivatives of avarol

The acetylcholinesterase inhibitory and/or antitumour activities of amino-, thio-and ester-derivatives of avarol selected were evaluated for the first time at in vitro conditions. Avarol-3',4'-dithioglycol (1) and avarol-4'-(3) mercaptopropionic acid (3) were shown to be the best inhibitors of the enzyme tested (0.50 mu g and IC50 0.05mM and 0.50 mg and IC50 0.12 mM, respectively), while 4'-tryptamine-avarone (9) and avarol-3'-(3) mercaptopropionic acid (2) exhibited the highest cytotoxicity against the human breast T-47D cancer cell line (IC50 0.66 mu g/mL and 1.25 mu g/mL, respectively). According to experimental data obtained, the sesquiterpenoid hydroquinone structure of bioactive avarol derivatives may inspire development of new pharmacologically useful substances to be used in the treatment of Alzheimer's disease and/or human breast tumour

Preliminarna analiza hemije masnih kiselina vrsta Kindbergia praelonga i Kindbergia stokesii (Brachytheciaceae)

Moss species of the family Brachytheciaceae, Kindbergia praelonga (Hedw.) Ochyra and Kindbergia stokesii (Turn.) Ochyra, were preliminarily analysed for their fatty acid composition with the aim of studying the chemical relationship of these two entities. Fatty acid methyl esters were examined by GC and GC MS in their methanol extracts. Thirteen fatty acids were identified. It is likely that the mosses are chemically distinguishable and should be treated as separate entities. However, additional chemical constituents of various moss samples, such as phenolic acids, their derivatives and flavonoids, must be also analyzed in order to support the re-examination of the relationship between these two species.Preliminarno je ispitivan sastav viših masnih kiselina dve mahovine iz familije Brachytheciaceae, Kindbergia praelonga (Hedw.) Ochyra i Kindbergia stokesii (Turn.) Ochyra, sa hemotaksonomskim ciljem. Ukupno je identifikovano 13 viših masnih kiselina GC i GC-MS analizom. Na osnovu dobijenih eksperimentalnih rezultata se može zaključiti da se navedene biljne vrste značajno hemijski razlikuju i da bi se mogle smatrati zasebnim entitetima ukoliko se to potvrdi i dodatnim analizama

A New Lichen Depsidone from L Obaria Pulmonaria

The continuation of our phytochemical survey of Lobaria pulmonaria (L.) Hoffm. (Lobariaceae), has led to the isolation and identification of a new lichen depsidone (1) characterised by the normal analytical and spectroscopic techniques. Unlike previously isolated depsidone (2) from the same species, the compound 1 has not showed activity in the acetylcholinesterase inhibition test on Thin-layer chromatography plate

Supplementary data for the article: Pejin, B.; Stanimirovic, B.; Vujovic, D.; Popovic Djordjevic, J.; Velickovic, M.; Tesevic, V. The Natural Product Content of the Selected Cabernet Franc Wine Samples Originating from Serbia: A Case Study of Phenolics. Natural Product Research 2016, 30 (15), 1762–1765. https://doi.org/10.1080/14786419.2015.1135148

Supplementary material for: [https://doi.org/10.1080/14786419.2015.1135148]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2301

An Insight Into Selected Properties of Merlot Wines Obtained from Three New Clone Candidates

Covering the period 2009-2012, the quality of Merlot wines obtained from three new clone candidates (namely Nos. 022, 025 and 029, respectively; fourth phase of clonal selection) developed in the Republic of Serbia was examined. The following parameters were determined: Folin-Ciocalteu (FC) index, pH value, total acidity, volatile acids, aldehydes, esters, relative density and ash. In comparison with the standard Merlot wine (originating from mother vine), FC index (from 35.73 to 37.15) and tasting score (from 17.89 to 17.95) were higher for the wines of all three clone candidates tested. These findings are in good agreement with the observed trend for the viticultural parameters indicating better properties of the new Merlot clone candidates versus mother