The fatty acid composition of the moss Calliergonella cuspidata (Hedw.) Loeske (Amblystegiaccae) was preliminary analyzed by gas chromatography (GC FID) and gas chromatography-mass spectrometry (GC-MS). Six fatty acids were identified in its chloroform-methanol extract 1/1: palmitic acid (66.83 %), stearic acid (11.73%), oleic acid (8.21 %), linolenic acid (6.01 %), alpha-linolenic acid (3.95 %) and arachidic acid (3.26 %). Arachidonic acid and cis-5,8,11,14,17-eicosapentaenoic acid, typical long-chain polyunsaturated fatty acids for many mosses, were not detected in Calliergonella cuspidata