THE EFFECT OF DIFFERENT EXTERNAL ELASTIC COMPRESSION ON MUSCLE STRENGTH, FATIGUE, EMG AND MMG ACTIVITY

The purpose of this study was to quantify the effects of three different compression conditions on (a) performance of muscle strength/power and fatigue in lower extremity, and (b) the responses of electromyography (EMG) and mechanomyography (MMG) of rectus femoris (RF) under repeated concentric muscle actions. All subjects (N=12) performed maximal voluntary contractions (MVC) and consecutive, maximal isokinetic knee extension movements at 60°/s & 300°/s velocities with three different compression conditions. The results indicated that local elastic compression of lower extremity, while not significant in improving isokinetic strength in short period, may have a positive effect on fatigue by helping maintain long-term force production through altering muscle activity in high-velocity of locomotion

KINEMATIC ANALYSIS OF THE SLIDING STOP IN WESTERN RIDING AT THE MALLORCA WESTERN REINING TROPHY 2006

The purpose of this study was to show the acceleration acting during the sliding stop. As data source we used a DVD of 10 finalists of the Mallorca Western Festivals 2006 (reining competition). These videos were analysed using the SIMI-Motion software. Additionally to the defined location on the horse and rider the reference points on the horse (saddle pad) and on the panel fence (advertising board) were digitised. With the help of the reference point the coordinates were determined and the acceleration was calculated. The maximum acceleration of the sliding stop in the running direction was mean=37.92 m/s² (SD=9.47). The vertical acceleration of the sliding stop at this time was mean=8.50 m/s² (SD=6.26).With an expected mass of horse and rider between 500 to 600 Kg, this acceleration will lead to a load between 11.6 KN and 37.6 KN. The conclusion is that the acting load during the sliding stop is comparable to load on the extremity during a gallop race. The question remains what are the effects of the sliding to the lower hind extremities of the horse and does it lead to injuries

Dynamic Behaviour of High Performance of Sand Surfaces Used in the Sports Industry

The sand surface is considered a critical injury and performance contributing factor in different sports, from beach volleyball to greyhound racing. However, there is still a significant gap in understanding the dynamic behaviour of sport sand surfaces, particularly their vibration behaviour under impact loads. The purpose of this research was to introduce different measurement techniques to the study of sports sand surface dynamic behaviour. This study utilised an experimental drop test, accelerometry, in-situ moisture content and firmness data, to investigate the possible correlation between the sand surface and injuries. The analysis is underpinned by data gathered from greyhound racing and discussed where relevant.</jats:p

Are we ready to detect nematode diversity by next generation sequencing?

In a Technical Advance article, Porazinska et al. (2009, Molecular Ecology Resources, 9, 1439-1450) assessed next generation sequencing (NGS ) as a method for metagenomic analysis of nematode diversity. We agree that NGS has great potential here. However, it is not an easy path to the successful implementation of NGS for environmental DNA analysis of nematodes. Here, we describe the method's limitations and discuss prospective research questions. For instance, only a few direct extraction kits are suitable for nematode DNA extraction from bulk samples without adaptation. They enable the analysis of extracellular nematode DNA . The most crucial and unresolved issue remains the limited availability of suitable primers.(VLID)3146417Version of recor

Movement asymmetries in horses presented for prepurchase or lameness examination

Background The increasing popularity of objective gait analysis makes application in prepurchase examinations (PPE) a logical next step. Therefore, there is a need to have more understanding of asymmetry during a PPE in horses described on clinical evaluation as subtly lame.Objectives The objective of this study is to objectively compare asymmetry in horses raising minor vet concerns in a PPE and in horses raising major vet concerns with that found in horses presented with subtle single-limb lameness, and to investigate the effect of age/discipline on the clinicians' interpretation of asymmetry on the classification of minor vet concerns in a PPE.Study Design Clinical case-series.Methods Horses presented for PPE (n = 98) or subjectively evaluated as single limb low-grade (1-2/5) lame (n = 24, 13 forelimb lame, 11 hindlimb lame), from the patient population of a single clinic, were enrolled in the study provided that owners were willing to participate. Horses undergoing PPE were assigned a classification of having minor vet concerns (n = 84) or major vet concerns (n = 14) based on findings during the dynamic-orthopaedic part of the PPE. Lame horses were only included if pain-related lameness was confirmed by an objective improvement after diagnostic analgesia exceeding daily variation determined for equine symmetry parameters using optical motion capture. Clinical evaluation was performed by six different clinicians, each with >= 8 years of equine orthopaedic experience. Vertical movement symmetry was measured using optical motion capture, simultaneously with the orthopaedic examination. Data were analysed using previously described parameters and mixed model analysis and least squares means were used to calculate differences between groups.Results There was no effect of age or discipline on the levels of asymmetry within PPE horses raising minor vet concerns. MinDiff and RUD of the head discriminated between forelimb lame and PPE horses raising minor vet concerns; MinDiff, MaxDiff, RUD of the Pelvis, HHDswing and HHDstance did so for hindlimb lameness. Two lameness patterns differentiated both forelimb and hindlimb lame from PPE horses with minor vet concerns: RUD Poll + MinDiff Withers - RUD Pelvis and RUD Pelvis + RUD Poll - MinDiff Withers. Correcting for vertical range of motion enabled differentiation of PPE horses with minor vet concerns from PPE horses with major vet concerns.Main Limitations Objective data only based on trot on soft surface, limited number of PPE horses with major vet concerns.Conclusions Combinations of kinematic parameters discriminate between PPE horses with minor vet concerns and subtly lame horses, though overlap exists

Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing

<p>Abstract</p> <p>Background</p> <p>Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD) is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample.</p> <p>Methods</p> <p>Serum or plasma samples from 98 control individuals and 54 breast cancer patients were evaluated upon silica membrane- or MBD affinity-based DNA isolation via qPCR targeting potential DNA methylation markers as well as by protein-microarrays for tumor-autoantibody testing.</p> <p>Results</p> <p>In control individuals, an average DNA level of 22.8 ± 25.7 ng/ml was detected applying the silica membrane based protocol and 8.5 ± 7.5 ng/ml using the MBD-approach, both values strongly dependent on the serum sample preparation methods used. In contrast to malignant and benign tumor serum samples, cell free DNA concentrations were significantly elevated in sera of metastasizing breast cancer patients. Technical evaluation revealed that serum upon MBD-based DNA isolation is suitable for protein-array analyses when data are consistent to untreated serum samples.</p> <p>Conclusion</p> <p>MBD affinity purification allows DNA isolations under native conditions retaining the protein function, thus for example enabling combined analyses of DNA methylation and autoantigene-profiles from the same serum sample and thereby improving minimal invasive diagnostics.</p

Novel Methods for Surface EMG Analysis and Exploration Based on Multi-Modal Gaussian Mixture Models

<div><p>This paper introduces a new method for data analysis of animal muscle activation during locomotion. It is based on fitting Gaussian mixture models (GMMs) to surface EMG data (sEMG). This approach enables researchers/users to isolate parts of the overall muscle activation within locomotion EMG data. Furthermore, it provides new opportunities for analysis and exploration of sEMG data by using the resulting Gaussian modes as atomic building blocks for a hierarchical clustering. In our experiments, composite peak models representing the general activation pattern per sensor location (one sensor on the long back muscle, three sensors on the gluteus muscle on each body side) were identified per individual for all 14 horses during walk and trot in the present study. Hereby we show the applicability of the method to identify composite peak models, which describe activation of different muscles throughout cycles of locomotion.</p></div