Organic Extracellular Matrix Components at the Bone Cell/Substratum Interface

We have recently shown that differentiating bone cells are capable of elaborating, in vitro, a calcified matrix at the interface with several non-biological substrata which is similar to cement lines found in remodelling bone tissue. From previous morphological observations, this matrix appeared free of assembled collagen fibres which indicated that osteogenic cells produce an initial mineralized matrix before overt collagen fibre production. In the work reported herein, we used six antibodies to extracellular matrix components and two conjugates of secondary antibody, fluorescein and colloidal gold, to initiate a preliminary characterization of the organic components at the bone cell substratum interface at early periods of culture. The labels were visualized by transmitted fluorescence light and immuno-gold scanning electron microscopy, respectively. Both secondary and backscattered modes were employed in the latter. The results showed that while chondroitin sulphate was ubiquitous in this culture system, osteopontin labeled discretely at the ends of cell pseudopodia, initially, following which the substratum surface was strongly labeled. The distribution of fibronectin was significantly different to that of osteopontin and indicated that this protein was primarily involved in cell/cell rather than cell/substratum adhesion. Although Type I collagen was not present in the extracellular matrix at early periods of culture, it was evident intracellularly at 3 days and extracellularly after the formation of the initial cement-like matrix. However, Type III collagen displayed the inverse sequence. In less mature cultures, it exhibited a ubiquitous distribution, while it was un-detectable at later stages of culture. Finally, osteocalcin was the only antibody employed which showed no labeling at early culture periods while at later stages, both the cellular compartment and the previously formed interfacial matrix labeled positively

The growth of structure in the Szekeres inhomogeneous cosmological models and the matter-dominated era

This study belongs to a series devoted to using Szekeres inhomogeneous models to develop a theoretical framework where observations can be investigated with a wider range of possible interpretations. We look here into the growth of large-scale structure in the models. The Szekeres models are exact solutions to Einstein's equations that were originally derived with no symmetries. We use a formulation of the models that is due to Goode and Wainwright, who considered the models as exact perturbations of an FLRW background. Using the Raychaudhuri equation, we write for the two classes of the models, exact growth equations in terms of the under/overdensity and measurable cosmological parameters. The new equations in the overdensity split into two informative parts. The first part, while exact, is identical to the growth equation in the usual linearly perturbed FLRW models, while the second part constitutes exact non-linear perturbations. We integrate numerically the full exact growth rate equations for the flat and curved cases. We find that for the matter-dominated era, the Szekeres growth rate is up to a factor of three to five stronger than the usual linearly perturbed FLRW cases, reflecting the effect of exact Szekeres non-linear perturbations. The growth is also stronger than that of the non-linear spherical collapse model, and the difference between the two increases with time. This highlights the distinction when we use general inhomogeneous models where shear and a tidal gravitational field are present and contribute to the gravitational clustering. Additionally, it is worth observing that the enhancement of the growth found in the Szekeres models during the matter-dominated era could suggest a substitute to the argument that dark matter is needed when using FLRW models to explain the enhanced growth and resulting large-scale structures that we observe today (abridged)Comment: 18 pages, 4 figures, matches PRD accepted versio

Pasture Management to Improve Dry Matter Intake

Agricultural producers are constantly looking for ways to maximize returns while reducing input costs. On dairy operations, a move from confinement feeding to pasture grazing offers the potential to reduce costs associated with harvest and storage of feed. In such a transition, producers sometimes report a decline in milk production and growth of livestock—both of which can strongly correlate to dry matter intake. Fortunately, dry matter intake is something that can be influenced by management practices. In this publication, we discuss the pasture management practices to improve dry matter intake

Interpreting and acting upon home blood pressure readings: A qualitative study

This article is made available through the Brunel Open Access Publishing Fund. Copyright @ 2013 Vasileiou et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: Recent guidelines recognize the importance of home blood pressure monitoring (HBPM) as an adjunct to clinical measurements. We explored how people who have purchased and use a home blood pressure (BP) monitor make sense of, and act upon, readings and how they communicate with their doctor about the practice of home monitoring. Methods: A qualitative study was designed and participants were purposively recruited from several areas in England, UK. Semi-structured in-depth interviews were conducted with 18 users of home BP monitors. The transcribed data were thematically analysed. Results: Interpretation of home BP readings is complex, and is often characterised by uncertainty. People seek to assess value normality using ‘rules of thumb’, and often aim to identify the potential causes of the readings. This is done by drawing on lay models of BP function and by contextualising the readings to personal circumstances. Based on the perceived causes of the problematic readings, actions are initiated, mostly relating to changes in daily routines. Contacting the doctor was more likely when the problematic readings persisted and could not be easily explained, or when participants did not succeed in regulating their BP through their other interventions. Most users had notified their doctor of the practice of home monitoring, but medical involvement varied, with some participants reporting disinterest or reservations by doctors. Conclusions: Involvement from doctors can help people overcome difficulties and resolve uncertainties around the interpretation of home readings, and ensure that the rules of thumb are appropriate. Home monitoring can be used to strengthen the patient-clinician relationship

Baryon acoustic oscillations from Integrated Neutral Gas Observations: Radio frequency interference measurements and telescope site selection

The Baryon acoustic oscillations from Integrated Neutral Gas Observations (BINGO) telescope is a new 40-m class radio telescope to measure the large-angular-scale intensity of Hi emission at 980-1260 MHz to constrain dark energy parameters. As it needs to measure faint cosmological signals at the milliKelvin level, it requires a site that has very low radio frequency interference (RFI) at frequencies around 1 GHz. We report on measurement campaigns across Uruguay and Brazil to find a suitable site, which looked at the strength of the mobile phone signals and other radio transmissions, the location of wind turbines, and also included mapping airplane flight paths. The site chosen for the BINGO telescope is a valley at Serra do Urubu, a remote part of Paraiba in North-East Brazil, which has sheltering terrain. During our measurements with a portable receiver we did not detect any RFI in or near the BINGO band, given the sensitivity of the equipment. A radio quiet zone around the selected site has been requested to the Brazilian authorities ahead of the telescope construction.Comment: Preprint of an article accepted in the Journal of Astronomical Instrumentation, copyright 2018 World Scientific Publishing Company https://www.worldscientific.com/worldscinet/ja

H0LiCOW XI. A weak lensing measurement of the external convergence in the field of the lensed quasar B1608+656 using HST and Subaru deep imaging

We investigate the environment and line of sight of the H0LiCOW lens B1608+656 using Subaru Suprime-Cam and the Hubble Space Telescope (HST) to perform a weak lensing analysis. We compare three different methods to reconstruct the mass map of the field, i.e. the standard Kaiser-Squires inversion coupled with inpainting and Gaussian or wavelet filtering, and $\tt{Glimpse}$ a method based on sparse regularization of the shear field. We find no substantial difference between the 2D mass reconstructions, but we find that the ground-based data is less sensitive to small-scale structures than the space-based observations. Marginalising over the results obtained with all the reconstruction techniques applied to the two available HST filters F606W and F814W, we estimate the external convergence, $\kappa_{\rm ext}$ at the position of B1608+656 is $\kappa_{\rm ext} = 0.11^{+0.06}_{-0.04}$, where the error bars corresponds respectively to the 16th and 84th quartiles. This result is compatible with previous estimates using the number-counts technique, suggesting that B1608+656 resides in an over-dense line of sight, but with a completely different technique. Using our mass reconstructions, we also compare the convergence at the position of several groups of galaxies in the field of B1608+656 with the mass measurements using various analytical mass profiles, and find that the weak lensing results favor truncated halo models.Comment: Accepted MNRA

Energy metrics to evaluate the energy use and performance of water main assets

Managing aging infrastructure has become one of the greatest challenges for water utilities, particularly when faced with selecting the most critical pipes for rehabilitation from among the thousands of candidates. This paper presents a set of novel yet practical energy metrics that quantify energy interactions at the spatial resolution of individual water mains to help utilities identify pipes for rehabilitation. The metrics are demonstrated using a benchmark system and two large, complex systems. The results show that the majority of pipes have good energy performance but that an important minority of outlier pipes have low energy efficiency and high energy losses due to friction and leakage. Pumping and tank operations tend to drive energy efficiency and energy losses in pipes close to water sources, whereas diurnal variation in demand drives energy performance of mains located far away from water sources. The new metrics of energy lost to friction and energy lost to leakage can provide information on energy performance in a pipe that is complementary to the traditional measures of unit head loss and leakage flow

Comparative fruit quality parameters of ‘Ninfa’ apricot (Prunus armeniaca L.) grafted on two different rootstocks in a newly established organic orchard

The fruit quality parameters of Prunus armeniaca L. cv ‘Ninfa’ grafted on ‘Myrobalan 29C’ (Prunus cerasifera Ehrh.) and ‘Real Fino’ apricot seedling (Prunus armeniaca L.) were analysed in an experimental orchard under organic management. The study was performed between 2010 and 2012 in the province of Seville (SW Spain). Colour, fruit and stone weights, firmness, soluble solid concentration, and acidity were measured for fruit quality evaluation. Trunk cross-sectional area, main branches, and fruit yield were also determined. In general, ‘Myrobalan 29C’ rootstock produced fruit slightly larger in size and with a bigger weight. By contrast, apricots on ‘Myrobalan 29C’ had less firmness and a lower solid soluble concentration than on ‘Real Fino’. There was little difference in the colour, acidity, and stone dry weights. Trees on ‘Real Fino’ had larger areas of trunk and branches but no significant differences were obtained in relation to fruit yields

We are all one together : peer educators\u27 views about falls prevention education for community-dwelling older adults - a qualitative study

Background: Falls are common in older people. Despite strong evidence for effective falls prevention strategies, there appears to be limited translation of these strategies from research to clinical practice. Use of peers in delivering falls prevention education messages has been proposed to improve uptake of falls prevention strategies and facilitate translation to practice. Volunteer peer educators often deliver educational presentations on falls prevention to community-dwelling older adults. However, research evaluating the effectiveness of peer-led education approaches in falls prevention has been limited and no known study has evaluated such a program from the perspective of peer educators involved in delivering the message. The purpose of this study was to explore peer educators’ perspective about their role in delivering peer-led falls prevention education for community-dwelling older adults. Methods: A two-stage qualitative inductive constant comparative design was used.In stage one (core component) focus group interviews involving a total of eleven participants were conducted. During stage two (supplementary component) semi-structured interviews with two participants were conducted. Data were analysed thematically by two researchers independently. Key themes were identified and findings were displayed in a conceptual framework. Results: Peer educators were motivated to deliver educational presentations and importantly, to reach an optimal peer connection with their audience. Key themes identified included both personal and organisational factors that impact on educators’ capacity to facilitate their peers’ engagement with the message. Personal factors that facilitated message delivery and engagement included peer-to-peer connection and perceived credibility, while barriers included a reluctance to accept the message that they were at risk of falling by some members in the audience. Organisational factors, including ongoing training for peer educators and formative feedback following presentations, were perceived as essential because they affect successful message delivery. Conclusions: Peer educators have the potential to effectively deliver falls prevention education to older adults and influence acceptance of the message as they possess the peer-to-peer connection that facilitates optimal engagement. There is a need to consider incorporating learnings from this research into a formal large scale evaluation of the effectiveness of the peer education approach in reducing falls in older adults