Caracterización de los mecanismos que median la muerte celular inducida por diferentes tipos de acetogeninas de annonacaeas en líneas tumorales humanas

Tesis doctoral inédita. Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 11/2/201

Bio-organic Alternatives for Propagation of Psidium Guajava (L.) through Stem Cuttings

The aim of this study was to use different stimulators for guajabo propagation using stem cuttings in semi-controlled conditions, on "La Nueva Esperanza" farm, run by "Renato Guitart" Strengthened Cooperative of Credits and Services, in the municipality of Camaguey, Cuba, between February and May, 2016. A chamber with zeolite substrate (1 - 3 mm diameter) was used for propagation, whereas irrigation was scheduled and localized. A completely randomized design was used for evaluation, with 6 treatments and 3 replicas. The number of plants with buds, plants with roots, leaves, and live plants achieved, were evaluated. Simple variance analysis (SSPS version 11.5.) was performed, and the means were compared according to the Duncan´s test (p:0.05). The bio-organic alternatives applied were efficient in stimulating the physiological mechanisms for plantlet growth. The best phenological behavior was observed in treatments with the natural liquid humus plus indoleacetic acid, the natural liquid humus plus phosphoric acid, and the fortified liquid humus.&nbsp

Application of Bio-stimulants in Banana Vitroplant Production (Musa sp.)

This research took place between February and April 2016, at the Center of Vitroplant Acclimatization of the Bio factory run by the Seed Company of Camaguey, in order to apply different bio-stimulants and evaluate the growth indicators observed during implementation of bio-organic alternatives to banana (Musa sp.) vitroplant production Polyethylene bags were used in the experiment, and the substrate contained 50% humus and 50% compost. The FHIA-03 variety was planted, following a completely randomized design with four treatments and four replicas. Natural Liquid Humus (NLH), Improved Liquid Humus (ILH), and Fortified Liquid Humus (FLH) were used as bio-organic products. Plant size, stem thickness, and leaf quantity were measured. All the data were statistically processed through simple variance analysis (ANOVA) to determine the significant differences. The Duncan multiple range test (P≤0.05), with 95% significance, was used for comparison of means. Data variance analysis was performed by SSPS, version 11.5.1, for Windows. The Duncan multiple range test (p:0.05) was performed when significant differences were observed. The application of bio-organic products containing FLH produced the highest benefits to crops for the indicators evaluated. 

Cuestionario de Fallos de Memoria de la Vida Cotidiana (MFE). Análisis de factores con población española.

Uno de los instrumentos más utilizados para valorar los olvidos cotidianos es el Cuestionario de Fallos de Memoria de la Vida Cotidiana (MFE). Los objetivos del presente estudio fueron: a) examinar la estructura factorial del MFE, b) explorar las relaciones de los factores resultantes con el rendimiento objetivo de memoria (Listas de Palabras y Escenas de la Escala de Memoria de Wechsler - III), el estado de ánimo y la ansiedad (Escala de Depresión y Ansiedad de Goldberg) y con dos preguntas generales sobre quejas de memoria. Los datos se recogieron de una muestra de 647 adultos jóvenes (19-64 años). Los resultados mostraron la existencia de una estructura de tres factores, que explicaban el 29.3 % de la varianza: Recuerdo de Actividades, Monitorización de la Comunicación y Reconocimiento. Los dos primeros factores mostraron una correlación positiva más alta entre ellos y una asociación mayor con el estado de ánimo y las quejas generales de memoria. Por el contrario, el factor Reconocimiento presentó una correlación positiva más alta con la edad y negativa con el rendimiento objetivo de memoria

Effect of Bio-Organic Alternatives in the Agronomic Response of Onion on Los Angeles Farm

Context: Onion (Allium cepa L.) is an edible bulb that ranks, as a green vegetable, third in terms of harvested area. In general terms, world production of this crop is made intensively, with a broad application of agrochemicals, which threatens agricultural sustainability, health, and protection of the environment. Aim: To evaluate the effect of bio-organic alternatives in the agronomic response of onion. Methods: A randomized block experimental design with seven treatments was used (absolute control and samples with natural liquid humus, improved liquid humus, fortified liquid humus, fortified liquid humus, plus Phosphoric inducer BayFolan Forte, FitoMas E), and four repetitions. The treatments were made every seven days, starting seven days after the plantation of bulbs. Plant height, pseudostem thickness, leaf number, bulb diameter, and crop yield were the indicators evaluated. The main economic indicators of each treatment were determined. Costs, income, profit, and economic effect were calculated. Results: The results show the positive effect of the morphophysiological and yield indicators related to the application of different bio-organic alternatives. The study produced greater profits than the control. Conclusions: The application of bio-organic alternatives showed a positive effect on the morphophysiological and yield indicators evaluated. The fortified liquid humus plus the phosphoric inducer showed the best results, producing the highest profits in the financial assessment

Bioorganic Nutritional Alternatives in Carrots (Daucus carota var. sativa), under Intensive Gardening

Context: One oftoday´s challenges is to provide food to all inhabitants, taking into account the growth of global population, especially into the cities. Accordingly, the urban farming systems are important, as they offer a real solution in face of the people’s demands. Objective: To compare different bioorganic nutritional alternatives and their influence on growth and yield indicators in carrots (Daucus carota var. sativa). Methods: This research was done at Ernesto Che Guevara intensive green garden (UEB No. 2), from the Agricultural Company of Esmeralda municipality, between December 2016 and March 2017, on non-gley plastic dark soil with high fertility. A completely randomized experimental design was applied, with five treatments and three replicas, the indicators evaluated were plant height, root length, and yield. Results: The best agronomic indicators were achieved in the treatments based on fortified liquid humus enriched with boron, calcium, and zinc, which did not differ among them, but were different from the natural liquid humus, and the control. The fortified liquid humus produced the highest yields and the best economic results. Conclusions: A favorable response was observed to bioorganic alternatives in terms of plant growth and yields. The fortified liquid humus had the best economic effect, with a remarkable increase in revenues, compared to the control

Alternativas bioorgánicas en la multiplicación por esquejes de Psidium guajava (L.)

Con el objetivo, de emplear diferentes estimuladores para la reproducción del guayabo por esquejes en condiciones semicontroladas, la investigación se realizó en la finca la Nueva Esperanza, perteneciente a la Cooperativa de Créditos y Servicios Fortalecida Renato Guitart del municipio Camagüey, en el período comprendido de febrero a mayo del 2016. Se contó con una cámara para la reproducción de posturas del guayabo por esquejes, la cual posee riego por microjet programado; el soporte utilizado es la zeolita con diámetro de 1 a 3 mm, empleándose un diseño completamente aleatorizado con 6 tratamientos y 3 repeticiones cada uno. Se realizó la evaluación de plantas con gemas y sin gemas, plantas con raíces, número de hojas y plantas vivas en el cultivo de la guayaba. Para el análisis estadístico, se utilizó el SSPS versión 11.5.1 con análisis de varianza de clasificación simple, aplicando los rangos múltiples de Duncan para un nivel de significación de 0,05 %. Las alternativas bioorgánicas empleadas, determinaron eficiencia positiva en los mecanismos fisiológicos en el desarrollo de las plántulas, los tratamientos que mejor comportamiento fenológico fueron el humus líquido natural más ácido indolacético, humus líquido natural más ácido fosfórico y el humus líquido fortificado

Aplicación de bioestimulantes a vitroplantas de plátano (Musa sp. )

La investigación se realizó durante los meses de febrero - abril del 2016, en áreas del Centro de Aclimatación de vitroplantas de la Biofábrica, perteneciente a la Empresa de Semillas de Camagüey, con el objetivo de aplicar diferentes bioestimulantes y evaluar los indicadores de crecimiento que provengan de las aplicaciones de las alternativas bioorgánicas en el cultivo de vitroplantas de Plátano (Musa sp.). Para la realización del experimento se utilizaron bolsas de polietileno y el sustrato que se utilizó como soporte de las plantas estuvo conformado por un 50% de humus de lombriz y 50% de compost, se sembró la variedad FHIA-03, utilizándose un diseño completamente aleatorizado con cuatro tratamientos y cuatro repeticiones. Se emplearon el humus líquido natural (HLN), el humus líquido mejorado (HLM) y el humus líquido fortificado (HLF), como productos bio orgánicos, y se midió la altura de las plantas, grosor del tallo y el número de hojas. Los datos se sometieron al análisis de varianza mediante el paquete estadístico SSPS Versión 11.5.1 para Windows, y en el caso de  diferencias significativas se aplicó la prueba de rango múltiple de Duncan (p:0,05). La aplicación de los productos bio orgánicos compuestos por HLF logró los mayores beneficios para el cultivo en los indicadores evaluados.ABSTRACTThis research took place between February and April 2016, at the Center of Vitroplant Acclimatization of the Bio factory run by the Seed Company of Camaguey, in order to apply different bio-stimulants and evaluate the growth indicators observed during implementation of bio-organic alternatives to banana (Musa sp.) vitroplant production Polyethylene bags were used in the experiment, and the substrate contained 50% humus and 50% compost. The FHIA-03 variety was planted, following a completely randomized design with four treatments and four replicas. Natural Liquid Humus (NLH), Improved Liquid Humus (ILH), and Fortified Liquid Humus (FLH) were used as bio-organic products. Plant size, stem thickness, and leaf quantity were measured. All the data were statistically processed through simple variance analysis (ANOVA) to determine the significant differences. The Duncan multiple range test (P≤0.05), with 95% significance, was used for comparison of means. Data variance analysis was performed by SSPS, version 11.5.1, for Windows. The Duncan multiple range test (p:0.05) was performed when significant differences were observed. The application of bio-organic products containing FLH produced the highest benefits to crops for the indicators evaluated. 

Alternativas bioorgánicas en la multiplicación por esquejes de Psidium guajava (L.)

Con  el objetivo,  de emplear diferentes estimuladores para la reproducción del guayabo por esquejes en condiciones semicontroladas, la investigación se realizó en la finca la Nueva Esperanza, perteneciente a la Cooperativa de Créditos y Servicios Fortalecida Renato Guitart del municipio Camagüey, en el período comprendido de febrero a mayo del 2016. Se contó con una cámara para la reproducción de posturas del guayabo por esquejes, la cual posee riego por microjet programado; el soporte utilizado es la zeolita con diámetro de 1 a 3 mm, empleándose un diseño completamente aleatorizado con 6 tratamientos y 3 repeticiones cada uno. Se realizó la evaluación de plantas con gemas y sin gemas, plantas con raíces, número de hojas y plantas vivas en el cultivo de la guayaba. Para el análisis estadístico, se utilizó el SSPS versión 11.5.1 con análisis de varianza de clasificación simple, aplicando los rangos múltiples de Duncan para un nivel de significación de 0,05 %. Las alternativas bioorgánicas empleadas, determinaron eficiencia positiva en los mecanismos fisiológicos en el desarrollo de las plántulas, los tratamientos que mejor comportamiento fenológico fueron el humus líquido natural más ácido indolacético, humus líquido natural más ácido fosfórico y el humus líquido fortificado. ABSTRACT The aim of this study was to use different stimulators for guajabo scion-propagation in semi-controlled conditions, on "La Nueva Esperanza" farm, run by "Renato Guitart" Strengthened Cooperative of Credits and Services, in the municipality of Camaguey, Cuba, between February and May, 2016. A chamber for scion propagation with zeolite substrate (1 - 3 mm diameter) was used, irrigation was programmed and localized. A completely randomized design was used for evaluation, with 6 treatments and 3 replicas. The number of plants with buds, plants with roots, leaves, and live plants achieved, were evaluated. Simple variance analysis (SSPS version 11.5.) was performed, and the means were compared according to Duncan´s test (p:0.05). The bio-organic alternatives applied produced positive efficiency values in the physiological mechanisms of plantlet growth. The best phenological behavior was observed in treatments with the natural liquid humus plus indoleacetic acid, the natural liquid humus plus phosphoric acid, and the fortified liquid humus.&nbsp

Bio-organic Alternatives for Propagation of Psidium Guajava (L.) through Stem Cuttings

The aim of this study was to use different stimulators for guajabo propagation using stem cuttings in semi-controlled conditions, on "La Nueva Esperanza" farm, run by "Renato Guitart" Strengthened Cooperative of Credits and Services, in the municipality of Camaguey, Cuba, between February and May, 2016. A chamber with zeolite substrate (1 - 3 mm diameter) was used for propagation, whereas irrigation was scheduled and localized. A completely randomized design was used for evaluation, with 6 treatments and 3 replicas. The number of plants with buds, plants with roots, leaves, and live plants achieved, were evaluated. Simple variance analysis (SSPS version 11.5.) was performed, and the means were compared according to the Duncan´s test (p:0.05). The bio-organic alternatives applied were efficient in stimulating the physiological mechanisms for plantlet growth. The best phenological behavior was observed in treatments with the natural liquid humus plus indoleacetic acid, the natural liquid humus plus phosphoric acid, and the fortified liquid humus