Morphometric and phytochemical characterization and elevation effect on yield of three potato landraces of the Ligurian Apennines (Northern Italy)

The great adaptability, productivity and importance in human diet conditioned the diffusion of potato (Solanum tuberosum L.) in very different areas, leading to the formation of numerous landraces through anthropic or natural selection. Genoa mountainous inland is an historical area for potato cultivation and landraces are preserved by farmers associations as “Consorzio della Quarantina”. The aim of this study was the phytochemical and morphometric characterization of three potato landraces of the consortium (Quarantina Bianca, Quarantina Prugnona and Rubra spes) analysing the bio-agronomical performance at different elevations. The commercial cultivar Kennebec was used as control. For the morphometric analysis, the results of pairwise MANOVA shows that only Quarantina Bianca does not present significant differences with Kennebec in the mean shape. The four potato varieties resulted significantly different for the most of the considered phytochemical aspects (among content in ash, starch, solanine, total flavonoids, total phenols, chlorogenic acid, caffeic acid, ferulic acid and radical scavenging activity only starch and solanine content didn’t result statistically different). A remarkable result was a more consistent yield for all the varieties with the increase of elevation in the agronomical trials. We can conclude that Genovese landraces are a good choice to exploit Genovese mountain marginal territories

How harvest, cleaning and conservation good practices affect the quality of saffron: results of a research conducted in Italy

Saffron (Crocus sativus L.) spice making requires time-spending manual operations: stigmas are separated from the flower picked in bud early in the morning, and once dried they are preserved protected from light. This study verified how the correct pursuing of these good practices affects saffron quality. Few hours of exposure of the flower to the sun determined a significant decrease in the colouring strength (239.66 ± 10.33 versus 255.35 ± 11.87). The correct cleaning of stigmas determined a very significant increase of colouring strength (247.12 ± 13.32 instead of 224.35 ± 14.88) and a significant increase of flavour strength (99.72 ±7.48 against 90.31 ± 6.32, p <0.05). In 24 months, all the samples kept in the dark were still of first category of quality while the ones kept in the light dropped in second category. For all samples there was an increase of aroma strength and a decrease of the flavour and colouring strength, but only the colouring strength loss followed a significantly more sloping trendline. A detectable difference in the content of trans-crocin 4 correlated to the ageing or the way of conservation was not found, nevertheless, it was confirmed that the isomers tend towards a photostationary state where the trans isomer is more present

Multidisciplinary analysis of Italian Alpine wildflower honey reveals criticalities, diversity and value

Wildflower honeys produced in mountain grasslands are an expression of the biodiversity of these fragile habitats. Despite its importance, the botanical origin of honey is often defined without performing formal analysis. The aim of the study was to characterize six wildflower mountain honeys produced in the Italian Alps with different analytic techniques (SPME–GC–MS, HPLC-Orbitrap, cicatrizing and antioxidant activity) alongside melissopalynological analysis and botanical definition of the production area. Even though the apiaries were in mountain grasslands rich in Alpine herbaceous species, the honey could be defined as rhododendron/raspberry unifloral or raspberry and rhododendron bifloral while the honey produced at the lowest altitude differed due to the presence of linden, heather and chestnut. The non-compliance of the honey could be due to habitat (meadows and pastures) fragmentation, but also to specific compounds involved in the plant–insect relationship, such as kynurenic acid, present in a high quantity in the sample rich in chestnut pollen. 255 volatile compounds were detected as well as some well-known markers of specific botanic essences, in particular chestnut, linden and heather, also responsible for most of the differences in aroma profiling. A high correlation between nicotinaldehyde content and percentage of raspberry pollen (r = 0.853, p < 0.05) was found. Phenolic acid and hydroxy-fatty acid were predominant in the chestnut pollen dominant honey, which presented the highest antioxidant activity and the lowest cicatrizing activity, while the flavonoid fraction was accentuated in one sample (rhododendron pollen prevalent), that was also the one with the highest effect on wound closure, although all samples had similar cicatrizing effects apart from the chestnut pollen dominant honey (lowest cicatrizing activity). Our study highlighted the difficulty of producing mountain wildflower honey and the importance of a thorough characterization of this product, also to encourage its production and valorisation.Fil: Leoni, Valeria. Università degli Studi di Milano; ItaliaFil: Giupponi, Luca. Università degli Studi di Milano; ItaliaFil: Pavlovic, Radmila. Università degli Studi di Milano; ItaliaFil: Gianoncelli, Carla. Università degli Studi di Milano; ItaliaFil: Cecati, Francisco Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Ranzato, Elia. Università degli Studi di Milano; ItaliaFil: Martinotti, Simona. Università degli Studi di Milano; ItaliaFil: Pedrali, Davide. Università degli Studi di Milano; ItaliaFil: Giorgi, Annamaria. Università degli Studi di Milano; ItaliaFil: Panseri, Sara. Università degli Studi di Milano; Itali

The quinoa variety influences the nutritional and antioxidant profile rather than the geographic factors

Quinoa (Chenopodium quinoa Willd.) is attracting worldwide attention due to its nutritional and biological properties. Nowadays, this pseudocereal is cultivated worldwide in different environmental conditions. This work evaluates the nutritional profile, polyphenol content and antioxidant capacity of five quinoa cultivars (Negra Collana, Chullpi Real, Salcedo Inia, Pasankalla and Kancolla) from Spain and from the Andean region, looking for the key factor of quinoa composition. Nutritional profile was similar for the same cultivar among the locations but, protein and iron contents were higher (p < 0.05) in Spanish seeds compared to the Andean ones. PCA and Pearson correlation coefficient reveal that the darkest quinoa cultivars, Negra and Pasankalla, had the best bioactive profile because the greater dietary fiber, polyphenol content, and antioxidant capacity (p < 0.05), regardless of origin zone. Concluding, the genetic variability seems to have a higher influence than the geographic factors on the nutritional and antioxidant composition of quinoa

Binary Alginate-Whey Protein Hydrogels for Antioxidant Encapsulation

Encapsulation of antioxidants in hydrogels, i.e., three-dimensional networks that retain a significant fraction of water, is a strategy to increase their stability and bioaccessibility. In fact, low oxygen diffusivity in the viscous gelled phase decreases the rate of oxidation. Moreover, some hydrocolloids such as alginate and whey proteins provide a pH-dependent dissolution mechanism, allowing the retention of encapsulated compounds in the gastric environment and their release in the intestine, where they can be absorbed. This paper reviews the information on alginate-whey protein interactions and on the strategies to use binary mixtures of these polymers for antioxidant encapsulation. Results showed that alginate and whey proteins strongly interact, forming hydrogels that can be modulated by alginate molecular mass, mannuronic acid: guluronic acid ratio, pH, Ca2+ or transglutaminase addition. Hydrogels of alginate and whey proteins, in the forms of beads, microparticles, microcapsules, and nanocapsules, generally provide better encapsulation efficiency and release properties for antioxidants with respect to the hydrogel of alginate alone. The main challenges for future studies are to extend knowledge on the interactions among three components, namely alginate, whey proteins, and the encapsulated bioactive compounds, and to investigate the stability of these structures under food processing conditions. This knowledge will represent the rationale basis for the development of structures that can be tailored to specific food applications

Characterization and Future Distribution Prospects of “<i>Carciofo di Malegno</i>” Landrace for Its In Situ Conservation

“Carciofo di Malegno” is a little-known landrace of Cynara cardunculus subsp. scolymus cultivated in Camonica Valley (northern Italy). The morphological and phytochemical characteristics of this landrace were investigated; furthermore, a species distribution model (MaxEnt algorithm) was used to explore its ecological niche and the geographical area where it could be grown in the future. Due to its spiky shape, “Carciofo di Malegno” was distinct from any other artichoke sample considered, and it appears to be similar to those belonging to the “Spinosi” group. The concentration of chlorogenic acid (497.2 ± 116.0 mg/100 g DW) and cynarine (7.4 ± 1.2 mg/100 g DW) in “Carciofo di Malegno” was comparable to that of the commercial cultivars. In “Carciofo di Malegno,” luteolin was detected in a significant amount (9.4 ± 1.5 mg/100 g DW) only in the stems and in the edible parts of the capitula. A MaxEnt distribution model showed that in the coming decades (2040–2060s), the cultivation of this landrace could expand to the pre-Alps and Alps of Lombardy. Climate change may promote the diffusion of “Carciofo di Malegno”, contributing to preservation and the enhancement of this landrace and generating sustainable income opportunities in mountain areas through exploring new food or medicinal applications

The Importance of Technical Support in the Return of Traditional Crops in the Alps: The Case of Rye in Camonica Valley

Multifunctional agriculture could be strategic for the recovery of some mountain areas of the Alps, and traditional crops like cereals generated study cases that triggered processes of development, such as rye in Camonica Valley (Northern Italy). However, farmers are often newcomers, and the specificities of low input agriculture make the training in agriculture fundamental. The impact of public workshops/seminars (organized by the Ge.S.Di.Mont. Research Centre of the University of Milan in Camonica Valley) on cereal cultivation between 2016 and 2021 was investigated. Moreover, rye produced in Camonica Valley was analyzed. The results show an increase in participation and a wider use of the streaming service. The percentage of participants not from an agricultural background had always remained about 50%, but decreased to 15.17% &plusmn; 5.07 in 2021, in contrast to the increase of professionals in agriculture and forestry. This is probably due to the accreditation of training activities for agronomists and foresters, and to the start of specific training projects regarding cereals. Samples of rye produced in Camonica Valley following the period of training activities were phytochemically/nutritionally characterized and compared to commercial rye. Locally produced rye proved to be comparable to the commercial one; however it showed a remarkable unevenness in secondary metabolite content and productivity, due to environmental differences and diverse agro-techniques

Nutritional Characterization and Novel Use of &ldquo;Copafam&rdquo; Bean (Phaseolus coccineus L.) for the Sustainable Development of Mountains Areas

Agrobiodiversity conservation includes strategies and actions to be taken to prevent landrace loss, a worldwide problem. Landraces are local varieties that have agricultural, cultural, and historical value but most of these are not studied yet. This research aimed to study the nutritional and phytochemical characteristics of the &ldquo;Copafam&rdquo; bean. In addition, the sensory properties and consumers&rsquo; hedonic ratings in a model food formulation (biscuits) made by this landrace have been examined. The results show that &ldquo;Copafam&rdquo; had a high dietary fiber content (34.83 &plusmn; 2.48 g/100 g dw) and it resulted in a great source of secondary metabolites as polyphenols (121.36 &plusmn; 5.31 mg GAE/g dw), flavonoids (6.51 &plusmn; 0.17 mg/kg dw), and anthocyanins (28.11 &plusmn; 0.16 mg Cy3 G/kg dw), having remarkable antioxidant activity too. Biscuits made from &ldquo;Copafam&rdquo; bean flour were characterized by a darker color and crunchy texture, and it was considered acceptable by consumers. All these characteristics make it a resource of great interest for innovative forms of consumption like fortified foods. This research showed that landraces can represent a great resource for an innovative food industry aiming to preserve agrobiodiversity and promote the sustainable development of mountain areas

Nutritional Characterization and Novel Use of “Copafam” Bean (<i>Phaseolus coccineus</i> L.) for the Sustainable Development of Mountains Areas

Agrobiodiversity conservation includes strategies and actions to be taken to prevent landrace loss, a worldwide problem. Landraces are local varieties that have agricultural, cultural, and historical value but most of these are not studied yet. This research aimed to study the nutritional and phytochemical characteristics of the “Copafam” bean. In addition, the sensory properties and consumers’ hedonic ratings in a model food formulation (biscuits) made by this landrace have been examined. The results show that “Copafam” had a high dietary fiber content (34.83 ± 2.48 g/100 g dw) and it resulted in a great source of secondary metabolites as polyphenols (121.36 ± 5.31 mg GAE/g dw), flavonoids (6.51 ± 0.17 mg/kg dw), and anthocyanins (28.11 ± 0.16 mg Cy3 G/kg dw), having remarkable antioxidant activity too. Biscuits made from “Copafam” bean flour were characterized by a darker color and crunchy texture, and it was considered acceptable by consumers. All these characteristics make it a resource of great interest for innovative forms of consumption like fortified foods. This research showed that landraces can represent a great resource for an innovative food industry aiming to preserve agrobiodiversity and promote the sustainable development of mountain areas