Participants\u27 Perceptions of the Childcare Subsidy System

This paper presents a focus group study of perceptions of cash assistance participants in Cuyahoga County, Ohio and the San Fernando Valley in California regarding childcare subsidy use, choices of care, and perceptions of quality. TANF participants discuss experiences in the subsidy system and indicate needs and preferences for childcare. Advocates, policy makers, and parents recognize the need for suitable childcare so that TANF recipients can go to work. However, discussants\u27 comments demonstrate one result of a changing, but not yet changed, social safety net. The authors explore strategies to address participants\u27 concerns-childcare systems that neither function as promised, nor offer quality of care that enhances child development and is safe and comforting for children

Romanian Adoptees and Pre-adoptive Care: A Strengths Perspective

Ninety-one parents of 120 adopted Romanian children who were part of a longitudinal study were surveyed to determine perceptions of their relationships with their children, their adoption experiences, and the children\u27s strengths. The Behavioral and Emotional Rating Scale (BERS) was used and parents were easily able to rate their children\u27s emotional and behavioral strengths. Levels of parent-child relationship satisfaction were the most consistent predictors of child emotional and behavioral strength. The child\u27s age at the time of the survey was a significant predictor of strengths, indicating overall that younger children had more perceived strengths. Parents identified fewer strengths among children with longer histories of institutional care. The importance of a strong parent-child relationship and resilience after a history of early institutional care are discussed

Despite slow catalysis and confused substrate specificity, all ribulose bisphosphate carboxylases may be nearly perfectly optimized

The cornerstone of autotrophy, the CO(2)-fixing enzyme, d-ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), is hamstrung by slow catalysis and confusion between CO(2) and O(2) as substrates, an “abominably perplexing” puzzle, in Darwin's parlance. Here we argue that these characteristics stem from difficulty in binding the featureless CO(2) molecule, which forces specificity for the gaseous substrate to be determined largely or completely in the transition state. We hypothesize that natural selection for greater CO(2)/O(2) specificity, in response to reducing atmospheric CO(2):O(2) ratios, has resulted in a transition state for CO(2) addition in which the CO(2) moiety closely resembles a carboxylate group. This maximizes the structural difference between the transition states for carboxylation and the competing oxygenation, allowing better differentiation between them. However, increasing structural similarity between the carboxylation transition state and its carboxyketone product exposes the carboxyketone to the strong binding required to stabilize the transition state and causes the carboxyketone intermediate to bind so tightly that its cleavage to products is slowed. We assert that all Rubiscos may be nearly perfectly adapted to the differing CO(2), O(2), and thermal conditions in their subcellular environments, optimizing this compromise between CO(2)/O(2) specificity and the maximum rate of catalytic turnover. Our hypothesis explains the feeble rate enhancement displayed by Rubisco in processing the exogenously supplied carboxyketone intermediate, compared with its nonenzymatic hydrolysis, and the positive correlation between CO(2)/O(2) specificity and (12)C/(13)C fractionation. It further predicts that, because a more product-like transition state is more ordered (decreased entropy), the effectiveness of this strategy will deteriorate with increasing temperature