Analytical techniques for the study of soft contact lens spoilation

Soft contact lens wear has become a common phenomenon in recent times. The contact lens when placed in the eye rapidly undergoes change. A film of biological material builds up on and in the lens matrix. The long term wear characteristics of the lens ultimately depend on this process. With time distinct structures made up of biological material have been found to build up on the lens. A fuller understanding of this process and how it relates to the lens chemistry could lead to contact lenses that are better tolerated by the eye. The tear film is a complex biological fluid, it is this fluid that bathes the lens during wear. It is reasonable to suppose that it is material derived from this source that accumulates on the lens. To understand this phenomenon it was decided to investigate the make up and conformation of the protein species that are found on and in the lens. As inter individual variations in tear fluid composition have been found it is important to be able to study the proteins on a single lens. Many of the analytical techniques used in bio research are not suitable for this study because of the lack of sensitivity. Work with poly acrylamide electrophoresis showed the possibility of analyzing the proteins extracted from a single lens. The development of a biotin avidin electro-blot and an enzyme linked aniibody electro-blot, lead to the high sensitivity detection and identification of the proteins present. The extraction of proteins from a lens is always incomplete. A method that analyses the proteins in situ would be a great advancement. Fourier transform infra red microscopy was developed to a point where a thin section of a contact lens could yield information about the proteins present and their conformation. The three dimensional structure of the gross macroscopic structures termed white spots was investigated using confocal laser microscopy

Phase Behavior of Aqueous Na-K-Mg-Ca-CI-NO3 Mixtures: Isopiestic Measurements and Thermodynamic Modeling

A comprehensive model has been established for calculating thermodynamic properties of multicomponent aqueous systems containing the Na{sup +}, K{sup +}, Mg{sup 2+}, Ca{sup 2+}, Cl{sup -}, and NO{sub 3}{sup -} ions. The thermodynamic framework is based on a previously developed model for mixed-solvent electrolyte solutions. The framework has been designed to reproduce the properties of salt solutions at temperatures ranging from the freezing point to 300 C and concentrations ranging from infinite dilution to the fused salt limit. The model has been parameterized using a combination of an extensive literature database and new isopiestic measurements for thirteen salt mixtures at 140 C. The measurements have been performed using Oak Ridge National Laboratory's (ORNL) previously designed gravimetric isopiestic apparatus, which makes it possible to detect solid phase precipitation. Water activities are reported for mixtures with a fixed ratio of salts as a function of the total apparent salt mole fraction. The isopiestic measurements reported here simultaneously reflect two fundamental properties of the system, i.e., the activity of water as a function of solution concentration and the occurrence of solid-liquid transitions. The thermodynamic model accurately reproduces the new isopiestic data as well as literature data for binary, ternary and higher-order subsystems. Because of its high accuracy in calculating vapor-liquid and solid-liquid equilibria, the model is suitable for studying deliquescence behavior of multicomponent salt systems

A 2,500-year record of environmental change in Highlands Hammock State Park (Central Florida, USA) inferred from siliceous microfossils

Analysis of siliceous microfossils of a 79 cm long peat sediment core from Highlands Hammock State Park, Florida, revealed distinct changes in the local hydrology during the past 2,500 years. The coring site is a seasonally inundated forest where water availability is directly influenced by precipitation. Diatoms, chrysophyte statospores, sponge remains and phytoliths were counted in 25 samples throughout the core. Based on the relative abundance of diatom species, the record was subdivided into four diatom assemblage zones, which mainly reflect the hydrological state of the study site. An age-depth relationship based on radiocarbon measurements of eight samples reveals a basal age of the core of approximately 2,500 cal. yrs. BP. Two significant changes of diatom assemblage composition were found that could be linked to both, natural and anthropogenic influences. At 700 cal. yrs. BP, the diatom record documents a shift from tychoplanktonic Aulacoseira species to epiphytic Eunotia species, indicating a shortening of the hydroperiod, i.e. the time period during which a wetland is covered by water. This transition was interpreted as being triggered by natural climate change. In the middle of the twentieth century a second major turnover took place, at that time however, as a result of human impact on the park hydrology through the construction of dams and canals close to the study site

A large strain computational multi-scale model for the dissipative behaviour of wood cell-wall

This paper investigates the non-linear irreversible behaviour of wood cell-walls by means of a finite element-based computational multi-scale approach. A finite strain three-scale model is proposed where the overall response of the cell-wall composite is obtained by the computational homogenisation of a Representative Volume Element (RVE) of cell-wall material, whose mechanical response prediction, in turn, involves the computational homogenisation of a cellulose core–RVE. Numerical material tests are conducted with the proposed model. The results are compared to published experimental data and demonstrate the predictive capability of the proposed model in capturing key features of cell-wall behaviour, such as viscous relaxation, recovery mechanism and hysteresis. The present results suggest a failure mechanism for the cell-wall under straining which is associated with the inelastic yielding of the amorphous portion of cellulose fibres

Optimal recursive estimation of raw data

The original publication is available at www.springerlink.comWe present a new approach to the optimal estimation of random vectors. The approach is based on a combination of a specific iterative procedure and the solution of a best approximation problem with a polynomial approximant. We show that the combination of these new techniques allow us to build a computationally effective and flexible estimator. The strict justification of the proposed technique is provided.Anatoli Torokhti, Phil Howlett and Charles Pearc

The oldest (~ 1.9 Ga) metadolerites of the southern Siberian craton: age, petrogenesis, and tectonic setting

Geological, geochronological, and isotope-geochemical studies of the metadolerites of the Angaul complex, widespread in the Urik-Iya graben of the southern Siberian craton, were carried out. The metadolerites forming separate conformal bodies (sills) among the metasandstones of the Ingash Formation were studied in detail. U-Pb zircon (SHRIMP) dating of metadolerites yielded an age of 1913 ± 24 Ma, and U-Pb baddeleyite (ID-TIMS) dating of these rocks yielded an age of 1914.0 ± 1.7 Ma. Thus, the date of 1914 ± 2 Ma can be taken as the most precise age estimate for the studied rocks. The metadolerites of the Angaul complex correspond in chemical composition to the normal-alkaline tholeiitic basalts. Metadolerites are differentiated rocks with mg# of 36 to 58. They show fractionated REE patterns: (La/Yb)n = 1.2-3.5. All metadolerites, independently of their mg# value, have low contents of Nb (1.6-10.2 ppm) and show well-pronounced negative Nb-Ta anomalies in multielement patterns (Nb/Nb* = 0.19-0.54). The metadolerites are characterized by positive eNd(T) values ranging from 0.4 to 5.2, which correlate well with their SiO2 content and mg# value. The isotope-geochemical parameters of the metadolerites of the Angaul complex indicate that fractional crystallization, along with the assimilation of the host rocks (AFC), might have been the main process during the formation of the most differentiated metadolerites. The geochemical characteristics of metadolerites with the maximum mg# values of 57-58 and eNd(T) = 5.2 suggest that the parental mantle source of the metadolerites resulted from mixing of predominant depleted mantle material with the subcontinental-lithosphere material. Intrusion of the dolerites of the Angaul complex, as well as the deposition of the sedimentary strata of the Ingash Group, took place at the Paleoproterozoic stage of intracontinental extension caused by the collapse of the orogen resulted from the collision of the Biryusa block with the Tunguska superterrane in the southern Siberian craton

The Mencué Batholith: Permian episodic arc-related magmatism in the western North Patagonian Massif, Argentina

The Mencué Batholith, western North Patagonian Massif, includes three major bodies. The Mencué Granodiorite, Cura Lauquén Granite and La Blancura Syenogranite. There are dikes of granitic and basaltic rocks cutting the above-cited rocks. The Mencué Batholith represents several episodes of magmatism, with ages varying between 294 and 239 Ma. The Mencué Granodiorite and the Cura Lauquén Granite are solid-state deformed and are S-type. They have high-K and normal calc-alkaline affinities. These rocks contain significant quantities of subduction-zone chemical components that decrease towards younger lithofacies. La Blancura Syenogranite lack subduction zone chemical components and represent A-type granite, typical of within-plate magmatism. The partial melting of metapelites could be the process of formation of these bodies. The older lithofacies of the Mencué Batholith, found westernmost, display a stronger deformation, but there is a progressive eastward change to younger and mildly deformed bodies and even non-deformed bodies. We conclude that the evolution of the Mencué Batholith start in the Sakmarian-Roadian (Early Permian) period. At this time, a subduction zone was active to the west and its thermal influence affected sedimentary or metamorphic rocks producing S-type granites During the Wordian and Capitanian, (Middle Permian) periods, the Mencué Batholith was mildly deformed, possibly in the process of the vanishing of the deformation and has a minor subduction chemical signature. Between the Wuchiapingian and Olenekia periods, the alkaline facies of the Mencué Batholith show an absence of deformation and the characteristics of within-plate magmatism. The Early Permian magmatic events in the western North Patagonian Massif are represented by the older bodies of the Mencué Batholith and were produced by subduction in the western margin of the Gondwanan continent. The Late Permian-Early Triassic magmatic events show a noticeable decreasing influence of subduction and an increasing influence of within-plate chemical components.Fil: Gregori, Daniel Alfredo. Universidad Nacional del Sur. Departamento de Geología. Cátedra de Geología Argentina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Strazzere, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Barros, Mercedes Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Benedini, Leonardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Marcos, Paulo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; ArgentinaFil: Kostadinoff, Jose. Universidad Nacional del Sur. Departamento de Geología. Cátedra de Geología Argentina; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto Geológico del Sur. Universidad Nacional del Sur. Departamento de Geología. Instituto Geológico del Sur; Argentin