Desarrollo de análisis de imagen de dron en el rango visible 4K

Con el paso del tiempo y acentuándose tras situaciones como las vividas tras la pandemia, surge un crecimiento exponencial de la digitalización para llegar a un aumento de la eficiencia. La digitalización de la agricultura está en un proceso de crecimiento en España, con la finalidad de ahorrar recursos y mejorar el manejo del terreno. En la actualidad, tenemos dispositivos muy avanzados para poder explorar tanto los terrenos como recintos y extraer información sin apenas desplazamiento. Por ello, se han diseñado drones capaces de realizar todo esto ya sea de forma automatizada o con un posterior procesado de imágenes con programas externos. El interés de este trabajo es extraer características del terreno, agrícolas y ambientales, tomando imágenes a dos alturas en diferentes puntos usando un dron comercial de “usuario”, DJI Mavic Mini, con su correspondiente procesado de imágenes con la interfaz creada mediante códigos en el programa Matlab® y realizando un análisis en el rango visible 4K.Over the years and accentuating after situations such as those experienced after the pandemic, an exponential growth of digitization arises to reach an increase in efficiency. The digitization of agriculture is in a process of growth in Spain, in order to save resources and improve land management. Currently, we have very advanced devices to be able to explore both the land and the enclosures and extract information with hardly any movement. For this reason, drones capable of doing all this have been designed either automatically or with subsequent image processing with external programs. The interest of this work is to extract characteristics of the land, agricultural and environmental, taking images at two heights at different points using a commercial "user" drone, DJI Mavic Mini, with its corresponding image processing with the interface created by means of codes in the Matlab® program and performing an analysis in the 4K visible range.Universidad de Sevilla. Grado en Ingeniería de las Tecnologías de Telecomunicació

Pseudomonas pseudoalcaligenes CECT5344, a cyanide‑degrading bacterium with by‑product (polyhydroxyalkanoates) formation capacity

Background: Cyanide is one of the most toxic chemicals produced by anthropogenic activities like mining and jewelry industries, which generate wastewater residues with high concentrations of this compound. Pseudomonas pseudoalcaligenes CECT5344 is a model microorganism to be used in detoxification of industrial wastewaters containing not only free cyanide (CN−) but also cyano-derivatives, such as cyanate, nitriles and metal-cyanide complexes. Previous in silico analyses suggested the existence of genes putatively involved in metabolism of short chain length (scl-) and medium chain length (mcl-) polyhydroxyalkanoates (PHAs) located in three different clusters in the genome of this bacterium. PHAs are polyesters considered as an alternative of petroleum-based plastics. Strategies to optimize the bioremediation process in terms of reducing the cost of the production medium are required. Results: In this work, a biological treatment of the jewelry industry cyanide-rich wastewater coupled to PHAs production as by-product has been considered. The functionality of the pha genes from P. pseudoalcaligenes CECT5344 has been demonstrated. Mutant strains defective in each proposed PHA synthases coding genes (Mpha−, deleted in putative mcl-PHA synthases; Spha−, deleted in the putative scl-PHA synthase) were generated. The accumulation and monomer composition of scl- or mcl-PHAs in wild type and mutant strains were confirmed by gas chromatographymass spectrometry (GC–MS). The production of PHAs as by-product while degrading cyanide from the jewelry industry wastewater was analyzed in batch reactor in each strain. The wild type and the mutant strains grew at similar rates when using octanoate as the carbon source and cyanide as the sole nitrogen source. When cyanide was depleted from the medium, both scl-PHAs and mcl-PHAs were detected in the wild-type strain, whereas scl-PHAs or mcl-PHAs were accumulated in Mpha− and Spha−, respectively. The scl-PHAs were identified as homopolymers of 3-hydroxybutyrate and the mcl-PHAs were composed of 3-hydroxyoctanoate and 3-hydroxyhexanoate monomers. Conclusions: These results demonstrated, as proof of concept, that talented strains such as P. pseudoalcaligenes might be applied in bioremediation of industrial residues containing cyanide, while concomitantly generate by-products like polyhydroxyalkanoates. A customized optimization of the target bioremediation process is required to gain benefits of this type of approaches

SEGUNDA CAMPAÑA DE EXCAVACIONES (1983) EN EL POBLADO DE LA EDAD DEL COBRE DE "EL MALAGÓN" (CÚLLAR-BAZA, GRANADA)

La segunda campaña de excavación en el poblado de la Edad del Cobre de "El Malagón" (Cúllar-Baza, Granada) se ha realizado del 13 de junio al 15 de agosto de 1983, en cuanto a trabajos de excavación propiamente dichos, prolongándose con diversos trabajos de documentación hasta el 3 de septiembre, bajo la dirección de los profesores del Departamentode Prehistoria de Granada, Dres. D. Fernando Molina González y D. Francisco de la Torre Peña, siendo los responsables en las distintas áreas de excavación: D. Francisco Carrión Méndez y D. Francisco Contreras Cortés (profesores del mismo Departamento), D. Inocente Blanco de la Rubia, Da. Mª. Auxiliadora Moreno Onorato, D. Antonio Ramos Millán y Da. María del Pino de la Torre Santana (todos ellos Licenciados en Filosofía y Letras Opción Antigüedad, por la Universidad de Granada); D: José A. González Alcantud colaboró en los trabajos durante la primera semana de la campaña, trasladándose posteriormente a la excavación de urgencia que otro equipo realizaba simultáneamente en el cercano yacimiento argárico de "Castellón Alto", en Galera. Al margen de los citados, hay que reseñarla intervención en la campaña de Da. Inmaculada Rus (Universidad Complutense) y D. Roberto Santamaría (Universidad Autónoma de Madrid)

Identification and biochemical evidence of a medium-chain-length polyhydroxyalkanoate depolymerase in the Bdellovibrio bacteriovorus predatory hydrolytic Arsenal

The obligate predator Bdellovibrio bacteriovorus HD100 shows a large set of proteases and other hydrolases as part of its hydrolytic arsenal needed for its predatory life cycle. We present genetic and biochemical evidence that open reading frame (ORF) Bd3709 of B. bacteriovorus HD100 encodes a novel medium-chain-length polyhydroxyalkanoate (mcl-PHA) depolymerase (PhaZ Bd). The primary structure of PhaZ Bd suggests that this enzyme belongs to the α/β-hydrolase fold family and has a typical serine hydrolase catalytic triad (serine-histidine-aspartic acid) in agreement with other PHA depolymerases and lipases. PhaZ Bd has been extracellularly produced using different hypersecretor Tol-pal mutants of Escherichia coli and Pseudomonas putida as recombinant hosts. The recombinant PhaZ Bd has been characterized, and its biochemical properties have been compared to those of other PHA depolymerases. The enzyme behaves as a serine hydrolase that is inhibited by phenylmethylsulfonyl fluoride. It is also affected by the reducing agent dithiothreitol and nonionic detergents like Tween 80. PhaZ Bd is an endoexohydrolase that cleaves both large and small PHA molecules, producing mainly dimers but also monomers and trimers. The enzyme specifically degrades mcl-PHA and is inactive toward short-chain-length polyhydroxyalkanoates (scl-PHA) like polyhydroxybutyrate (PHB). These studies shed light on the potentiality of these predators as sources of new biocatalysts, such as an mcl-PHA depolymerase, for the production of enantiopure hydroxyalkanoic acids and oligomers as building blocks for the synthesis of biobased polymers.This work was supported by the Ministry of Economía y Competitividad (BIO2007-67304, BIO2010-21049, CSD2007-00005; CISC 201120E050) and by European Union Grants (NMP2-CT-2007-026515).Peer Reviewe

Yacimientos arqueológicos y artefactos. Las colecciones del Departamento de Prehistoria y Arqueología (I)

La serie editorial de Cuadernos Técnicos del Patrimonio surge debido a la necesidad de dotar al Vicerrectorado de Extensión Universitaria de publicaciones que aborden aspectos patrimoniales en relación con cuestiones de carácter transversal y que sirvan de vehículo de difusión y diálogo de las distintas colecciones que conforman el rico acervo universitario. El objetivo es convertir estos Cuadernos en un espacio de reflexión y debate sobre temas relacionados con la conservación, la restauración, la gestión, la difusión y la puesta en valor de los bienes muebles e inmuebles de la Universidad de Granada en toda su amplitud. No se plantean con un enfoque exclusivamente local pues su intención es abrirse a distintas problemáticas patrimoniales y convertirse en un instrumento que integre estudios de carácter nacional e internacional. Asimismo, entendemos que al Patrimonio hay que afrontarlo desde una perspectiva histórica pero también actual y en diálogo con la compleja realidad social

Evaluation of a quality improvement intervention to reduce anastomotic leak following right colectomy (EAGLE): pragmatic, batched stepped-wedge, cluster-randomized trial in 64 countries

Background Anastomotic leak affects 8 per cent of patients after right colectomy with a 10-fold increased risk of postoperative death. The EAGLE study aimed to develop and test whether an international, standardized quality improvement intervention could reduce anastomotic leaks. Methods The internationally intended protocol, iteratively co-developed by a multistage Delphi process, comprised an online educational module introducing risk stratification, an intraoperative checklist, and harmonized surgical techniques. Clusters (hospital teams) were randomized to one of three arms with varied sequences of intervention/data collection by a derived stepped-wedge batch design (at least 18 hospital teams per batch). Patients were blinded to the study allocation. Low- and middle-income country enrolment was encouraged. The primary outcome (assessed by intention to treat) was anastomotic leak rate, and subgroup analyses by module completion (at least 80 per cent of surgeons, high engagement; less than 50 per cent, low engagement) were preplanned. Results A total 355 hospital teams registered, with 332 from 64 countries (39.2 per cent low and middle income) included in the final analysis. The online modules were completed by half of the surgeons (2143 of 4411). The primary analysis included 3039 of the 3268 patients recruited (206 patients had no anastomosis and 23 were lost to follow-up), with anastomotic leaks arising before and after the intervention in 10.1 and 9.6 per cent respectively (adjusted OR 0.87, 95 per cent c.i. 0.59 to 1.30; P = 0.498). The proportion of surgeons completing the educational modules was an influence: the leak rate decreased from 12.2 per cent (61 of 500) before intervention to 5.1 per cent (24 of 473) after intervention in high-engagement centres (adjusted OR 0.36, 0.20 to 0.64; P < 0.001), but this was not observed in low-engagement hospitals (8.3 per cent (59 of 714) and 13.8 per cent (61 of 443) respectively; adjusted OR 2.09, 1.31 to 3.31). Conclusion Completion of globally available digital training by engaged teams can alter anastomotic leak rates. Registration number: NCT04270721 (http://www.clinicaltrials.gov)