7 research outputs found
Modificación de grafeno con cadenas de polisulfona e incorporación a matrices poliméricas. Evaluación de las propiedades y de la biocompatibilidad de los nanocomposites
Mención Internacional en el título de doctorLa presente tesis trata del estudio y diseño de nanocomposites de grafeno empleando
como matrices poliméricas polisulfona y una mezcla de polímeros de polisulfona y resina
epoxi.
En el diseño de estos nanocomposites juega un papel muy importante la interfase del
nanomaterial; ya que una buena interacción entre la nanopartícula y la matriz en la región
interfacial podría mejorar la estabilidad y despersabilidad de los nanorrefuerzos. Para
aumentar la compatibilidad con la matriz, se optó por modificar la superficie de grafeno
con cadenas de polifulfona. La modificación superficial del nanorrefuerzo se realizó
mediante el método que se conoce como nitrene chemistry empleando dos estrategias
sintéticas diferentes; permitiendo obtener cadenas de polímero ancladas con diferente peso
molecular (diferente longitud).
La polisulfona es un polímero empleado generalmente en aplicaciones biomédicas y
medioambientales, por lo que además, se estudió la biocompatibilidad de los
nanomateriales de rGO con cadenas de polisulfona. Para que un material sea
biocompatible debe cumplir dos requisitos, por un lado que presente propiedades
antibacterianas, y además, que no presente citotoxicidad. El estudio realizado de la
biocompatibilidad de nuestro nanomaterial muestra que este puede ser potencialmente
empleado en aplicaciones que requieran contacto directo con el ser humano, ofreciendo
además propiedades antibacterianas.
La preparación de los nanocomposites de polisulfona tuvo lugar mediante la técnica
de extrusión-inyección. Se prepararon nanocomposites con diferente carga de
nanomaterial, 0.1, 0.5, 1.0 y 3 % en peso de rGO y grafeno modificado con polisulfona. La dispersión del nanorrefuerzo presenta una notable importancia en las propiedades del xnanocomposite, por lo que previo al estudio de sus propiedades se analizó mediante
reología, espectroscopía dieléctrica y técnicas microscópicas su dispersión en la matriz
polimérica. De los resultados obtenidos, todas las técnicas empleadas para el estudio de la dispersión concluyen que la modificación superficial con cadenas de polisulfona de mayor peso molecular ayuda a mejorar la dispersabilidad.
Se estudiaron las propiedades mecánicas, térmicas y eléctricas de los nanocompostites, y además, se realizaron estudios de biodegradabilidad. Los resultados
muestran que al aumentar la carga de nanorrefuerzo, mejoran significativamente sus
propiedades térmicas y mecánicas, obteniéndose mejoras en los nanocomposites
modificados con cadenas largas de polisulfona. El estudio conformacional de las cadenas,
concluye que las cadenas de mayor peso molecular se encuentran preferentemente en
conformación semidiluida y además, se encuentran a una distancia lo suficientemente
alejadas unas de otras para que las cadenas de polisulfona de la matriz interpenetren en las cadenas de polisulfona ancladas a la superficie del rGO. Esta interpenetración ayuda a
mejorar y fortalecer la región interfacial, observándose en la mejora de las propiedades.
Los estudios de biodegradabilidad muestran que este nanomaterial, a altos contenidos de
rGO, es altamente resistente a periodos largos de exposición en contacto con aguas
residuales industriales, debido a que sus propiedades iniciales se mantienen y además, es
capaz de inhibir la formación de biofilms.
Los nanorrefuerzos sintetizados fueron también embebidos en una mezcla de
polímeros epoxi polisulfona, con el fin de obtener una morfología de fases invertidas. Se
pretendía obtener esta morfología para introducir las láminas de grafeno preferentemente
en los canales que forma la fase co-continua, para dar lugar a lo que se conoce como
fenómeno de doble percolación. Los resultados del análisis de las propiedades estudiadas
de estos nanocomposites muestran cómo el nanorrefuerzo modificado con cadenas de
polisulfona ayuda a obtener la morfología deseada, obteniendo resultados satisfactorios en las propiedades mecánicas y térmicas estudiadas.Polymer-matrix nanocomposites formed by incorporation of graphene sheets in polymer
matrices have attracted enormous attention in various fields of science and engineering, due to the excellent properties of graphene sheets. Specifically, graphene has been used to improve the mechanical, thermal, electrical, and barrier properties of polymers. Graphenebased polymer nanocomposites have been employed for diverse applications in electronics, aerospace, automotive manufacturing, and green energy.
Polysulfones (PSUs) are high-temperature thermoplastic polymers that exhibit great
chemical inertness, enhanced oxidative resistance, thermal and hydrolytic stability, as well
as high mechanical strength. Additionally, PSUs might be easily processed as a film and
thus, they are good candidates for different applications, such as gas separation,
hemodialysis, nano/ultra-filtration, adhesives for metal to metal bonds, membranes for fuel cells, drug delivery, or matrices for fiber reinforced composites.
Reduced graphene oxide (rGO) is of particular interest, since functional groups on its
surface enhance its solubility in organic solvents and remarkably facilitate surface
modification with organic molecules or polymers. The grafting of polymer brushes
improves the dispersion state and the polymer/graphene interfacial adhesion with the
subsequent increase in the nanocomposite properties. During the nanocomposite synthesis, both the rGO/polymer ratio and the molecular weight of the graft polymer play an
important role regarding rGO dispersion. This is because the behavior of polymer brushes is strongly determined by the polymer chain conformation. The grafting density and the
critical spacing between two neighboring chains determine the brush regime, i.e.
mushroom, crossover, and brush-like regimes. Depending on the conformation of the vigrafted
polymer chains, the interactions with the polymer matrix may be improved.
Another factor to be considered in order to enhance the interphase region in
nanocomposite materials is the preparation method of those. One of the objectives of this
thesis is improving some of these parameters to obtain better properties in the final
nanomaterial, studying the interphase of the system.
The present dissertation reports, for the first time, the modification of reduced
graphene oxide nanosheets with polysulfone chains through two different synthetic routes
via nitrene chemistry. The PSU polymer was bonded to rGO at the end (rGO-PSU end) or
randomly along the PSU chain (rGO-PSU mid). These strategies allowed to obtain
polymer brushes with two different lengths on the surface. The resulting rGO-PSU
synthetic products were carefully characterized by Raman, FTIR, XPS, TEM, and TGA,
evidencing the successful grafting of PSU onto rGO surfaces. The long-term stability of
these nanosheets was also determined in common solvents.
In addition, the biocompatibility of the nanoparticles was tested. This study involved
the antimicrobial properties and cytotoxicity in human cells. The nanoparticles of modified
and non-modified graphene were tested at different concentrations to determine the most
toxic concentrations to both, Gram-positive (B. subtilis) and Gram-negative (E. coli K12)
microorganisms. The results showed a reduction of 97% in the growth of B. subtilis after
three hours exposure, for the polymer modified nanosheets. The results also demonstrated that rGO-PSU mid exhibited better antimicrobial properties due to its shorter polymer
chains, which improves the contact of the microorganisms with the graphene surface.
Furthermore, cytotoxicity in human cells was evaluated, showing no toxic effect even at the
highest concentration employed in antimicrobial properties.
Unmodified and modified rGO with polysulfone brushes were included in
polysulfone and epoxy resin matrices to evaluate their properties. PSU nanocomposites
were prepared at four different percentages (up to 3 wt% rGO) by extrusion. The extruded
material was further processed by injection molding to finally obtain specimens for
evaluation of thermal, mechanical, electrical, and antimicrobial properties. The
morphology and microstructure of the prepared samples were examined by scanning
electron microscopy (SEM) and transmission electronic microscopy (TEM). Rheological
and dielectric spectroscopy were employed to study the dispersion state of the
nanocomposites, showing that nanocomposites with rGO-PSU end nanoparticles presented
better dispersion than non-modified graphene ones.
The results indicated that the extrusion-injection procedure was an efficient
preparation method of the nanocomposites with good dispersion degrees of rGO in the
matrix. Tensile test, dynamic mechanical thermal analysis (DMTA), and nanoindentation
showed an important improvement in the Young modulus respect to the neat polymer.
The enhancement of mechanical properties was interpreted in terms of the dispersion and
interface modification of rGO. A theoretical study about the polymer brushes
conformation at the interphase helped to understand the mechanical behavior. Thermal
properties of the nanocomposites were also analyzed, showing a moderate increase in the
thermal stability.
The antimicrobial properties of the prepared nanocomposites, with unmodified
graphene, were investigated in E. coli K12. Biofilm formation was studied by confocal
microscopy. The antimicrobial properties of rGO were preserved in the nanocomposites
and a decrease in the biofilm thickness was observed for the nanocomposites with 3 wt%
rGO. A biodegradation study was also performed by exposure to industrial wastewater for
nine days. The mechanical properties of the nanocomposites with high rGO content were
maintained and they exhibited antifouling properties. The results of this study showed that
these materials may be employed in environmental field applications.
Epoxy resins are widely used in many industry fields due to their inherent excellent
thermal and mechanical properties. Nevertheless, in order to increase toughness and meet
high performance applications, they are modified with elastomers, thermoplastics, and all sorts of nanoparticles embedded on the epoxy network. Modification with highperformance
engineering thermoplastic modifiers, such as polysulfones, helps to improve
epoxy resins toughness. Thermoplastics are usually partially miscible with epoxy resin
precursors at several temperatures and compositions, but as curing progress, the decrease
in the entropy of mixing leads to a two-phase structure by reaction-induced phase
separation (RIPS). Morphology and performance of epoxy/PSU blends has been
extensively studied. Several morphologies, such as sea-island, bicontinuous or doublephase,
and nodular (phase-inverted) structures have been observed.
In this work, rGO or rGO-PSU sheets, up to 1% by weight, have been incorporated
to an epoxy/PSU blend containing 20 wt% PSU by weight. The resulting epoxy
nanocomposites were characterized by differential scanning calorimetry (DSC), DMTA,
and rheology. The morphology and microstructure of the prepared samples were
examined by SEM. Phase-inverted morphologies (PSU as continuous phase) have been viiiobserved.
Nanocomposites showed interesting morphology changes in the presence of
rGO-PSU end, which may be due to rGO nanosheets were preferently embedded and
dispersed in PSU channels. The interest in this morphology lies in the possibility of
achieving the phenomena known as double threshold, introducing channels where
graphene sheets are preferably located. Nanocomposites with PSU and rGO-PSU end
nanoparticles showed also an improvement in the mechanical and thermal properties.
In summary, nitrene chemistry was successfully applied to graft PSU onto rGO
sheets. The synthetic strategy presented in this work demonstrates that modified graphene
nanosheets can be easily obtained in high yields. The resulting nanomaterials have suitable
dispersability and processability in organic solvents; and present improved antimicrobial
behavior and lower cytotoxicity compared to non-modified rGO.
PSU matrix nanocomposites show an improvement in mechanical, thermal,
antimicrobial, and degradability properties, and epoxy/PSU polymer blends modified with
rGO exhibit noticeable morphology and mechanical changes.Programa Oficial de Doctorado en Ciencia e Ingeniería de MaterialesPresidente: Mauricio Terrones.- Secretario: Juan Baselga Llido.- Vocal: Aránzazu del Campo Bécare
Biological Degradation and Biostability of Nanocomposites Based on Polysulfone with Different Concentrations of Reduced Graphene Oxide
Increasing incorporation of rGO in the polysulfone polymer generates materials with improved chemical and mechanical stability and less prone to biodegradation at the end of the nanocomposite life cycle. The results of attenuated total reflection infrared (ATR?IR) and mechanical strength, after exposure to wastewater influent, show that the increasing concentrations of rGO into the polymer matrix reduce changes in the nanocomposite properties. The increasing incorporation of rGO also increases growth inhibition of the wastewater microbial population on the surface of nanocomposites. Highest biofilm inhibition and material stability are observed with nanocomposites containing 3 wt% rGO. These results suggest that reduction in the material biodegradation is linked to the inhibition of biofilm growth on the nanocomposite surface due to the antimicrobial properties of rGO. This study demonstrates, for the first time, that the amount of rGO incorporated in the nanocomposite impact the biodegradability and end of life of polysulfone nanocomposites
New insights on the self-organization of PEG/alpha-cyclodextrin- based Polyrotaxanes in solution: an experimental and theoretical study
Size exclusion chromatography coupled with light scattering (SEC/MALS), dynamic light scattering (DLS), steady-state and time-resolved fluorescence, as well as molecular dynamics (MD) simulations are used to study the behavior of several poly(ethylene glycol) (PEG)/α-cyclodextrins (αCDs) polyrotaxanes (PRs) in solution. The number of CD units in any of the PRs studied is always smaller than that required to saturate the PEG chains. These PRs seem to aggregate in dimethyl sulfoxide (DMSO) solution. The presence of hairpins in the non-saturated PRs contributes to diminishing their expected large dimensions. Intra- and intermolecular interactions and forces responsible for hairpins and aggregation are investigated.This work was supported by the University of Alcalá (GC2011–002 and UAH2011/EXP-036) and Nanomod (MAT2010–17091) from the Spanish Ministerio de Ciencia e Innovación. The authors acknowledge the assistance of M. L. Heijnen with the preparation of the manuscript
Functionalization of reduced graphene oxide with polysulfone brushes enhance antibacterial properties and reduce human cytotoxicity
The present study reports two routes to modify reduced graphene oxide (rGO) nanosheets with polysulfone (PSU) brushes via nitrene chemistry. The PSU polymer is bonded to rGO at the extremity (rGO-PSU end) and at the middle of the PSU chain (rGO-PSU mid). The resulting rGO-PSU synthetic products are carefully characterized by Raman and FTIR spectroscopy, XPS, TEM, and thermogravimetric analysis, evidencing the successful grafting of PSU onto rGO surfaces. The long-term stability of these nanosheets is also determined in common solvents. The antibacterial properties of polymer-functionalized rGO against the planktonic Bacilus subtilis and Escherichia coli are also investigated. It is established that the antimicrobial properties of these nanocomposites are due to the production of reactive oxygen species. The results also demonstrate that rGO-PSU mid presents better antimicrobial properties due to shorter polymer chains, which improves the contact of the microorganisms with the graphene surface.This work was financially supported by the Spanish Ministry of Economy and Competitiveness (MAT2014-57557-R), and partially supported by the U.S. National Science Foundation Career Award (NSF Award #104093). R.O. would like acknowledge support from U.S. National Science Foundation (CMMI-1538730 and DUE-CMMI-1538730/1003574). Janire Peña wants to acknowledge mobility grant from Carlos III University and Instituto Tecnológico de Química y Materiales “Alonso Barba”. Authors acknowledge Dr. A. Esteban-Arranz for giving access to ATR equipment
Prospective Clinical Evaluation of Intra-Articular Injection of Tin-117m (Sn) Radiosynoviorthesis Agent for Management of Naturally Occurring Elbow Osteoarthritis in Dogs: A Pilot Study
Poor bioavailability of antibiotics, toxicity, and development of antibiotic-resistant bacteria jeopardize antibiotic treatments. To circumvent these issues, drug delivery using nanocarriers are highlighted to secure the future of antibiotic treatments. This work investigated application of nanocarriers, to prevent and treat bacterial infection, presenting minimal toxicity to the IPEC-J2 cell line. To accomplish this, polymer-based nanoparticles (NPs) of poly(lactide-co-glycolide) (PLGA) and lignin-graft-PLGA (LNP) loaded with enrofloxacin (ENFLX) were synthesized, yielding spherical particles with average sizes of 111.8 ± 0.6 nm (PLGA) and 117.4 ± 0.9 nm (LNP). The releases of ENFLX from PLGA and LNP were modeled by a theoretical diffusion model considering both the NP and dialysis diffusion barriers for drug release. Biocompatible concentrations of ENFLX, enrofloxacin loaded PLGA(Enflx) and LNP(Enflx) were determined based on examination of bacterial inhibition, toxicity, and ROS generation. Biocompatible concentrations were used for treatment of higher- and lower-level infections in IPEC-J2 cells. Prevention of bacterial infection by LNP(Enflx) was enhanced more than 50% compared to ENFLX at lower-level infection. At higher-level infection, PLGA(Enflx) and LNP(Enflx) demonstrated 25% higher prevention of bacteria growth compared to ENFLX alone. The superior treatment achieved by the nanocarried drug is accredited to particle uptake by endocytosis and slow release of the drug intracellularly, preventing rapid bacterial growth inside the cells