Pluronic F-127 hydrogel for stem cell research: a bibliometric analysis using Scopus database

Stem cell research holds immense promise in regenerative medicine. However, the successful utilization of stem cells relies on their inherent properties and the appropriate support matrix that provides an optimal environment for growth and differentiation. Optimizing their delivery and retention at the target site is crucial to enhance stem cell-based therapies' effectiveness. In recent years, hydrogels have emerged as a popular choice for culturing and delivering stem cells due to their unique properties, including biocompatibility, tunable physical and chemical characteristics, and mimicking the native extracellular matrix. Among the various hydrogels available, Pluronic F-127 (PF-127) has gained significant attention in stem cell research. This paper aims to study the publication trends of research that discuss the utilization of PF-127 hydrogel for stem cell research. The analysis is based on data extracted from the Scopus database using bibliometric methods. The results revealed the publication trends, collaboration patterns among authors and institutions, research areas, influential journals, funding agencies, and thematic connections in this field. By understanding the current state of research and identifying key areas of focus, this analysis provides valuable insights for researchers and practitioners interested in harnessing the potential of PF-127 hydrogel in regenerative medicine and tissue engineering

Evaluation of Xylazine, Acepromazine and Medetomidine with Ketamine for General Anaesthesia in Rabbits

A randomized, prospective, blinded experimental study was conducted in 32 rabbits of either sex to compare  the anaesthetic and physiological effects of ketamine with different pre-anaesthetics. Rabbits were  randomly divided into 4 equal groups. Xylazine 6 mg/kg in animals of group xylazine-ketamine (XK), acepromazine  2 mg/kg in animals of group acepromazine-ketamine (AK), medetomidine 125 μg/kg in group  medetomidine-ketamine 1 (MK1) or medetomidine 250 μg/kg in group medetomidine-ketamine 2 (MK2)  were administered by intramuscular injection (IM). Five minutes later, ketamine 60 mg/kg was administered  intramuscularly to all the groups. The rabbits were observed for the onset of weak time, down time,  the time to loss of righting reflex, pedal reflexes and response to surgical stimuli. Heart rate, respiratory  rate and rectal temperature and arterial oxygen saturation of haemoglobin (SpO2) were recorded up to 60  min. Weak time, down time and time to loss of righting reflex were the shortest in animals of group MK2  as compared to the other groups. Pedal reflexes remained intact in all the animals of XK group, but were  abolished in 50% of the AK group, 75% of the MK1 group and 100% of animals in the MK2 group. Pain  was evinced during surgery by all the animals in group XK, 5 animals in group AK and 4 animals in group  MK1. The best analgesia was achieved in the animals of group MK2, where none of the animals showed  pain on surgical stimulation. Heart rate and SpO2 decreased significantly (P<0.01) in the animals of groups  XK, MK1 and MK2 but respiratory rate and rectal temperature decreased significantly (P<0.01) in all the  groups. However, all the animals recovered from anaesthesia without complications. It was concluded that  medetomidine 250 µg/kg and ketamine 60 mg/kg produced excellent anaesthesia to allow pain free surgery  and may be considered suitable for anaesthesia in New Zealand White rabbits.

Evaluation of midazolam-ketamine with dexmedetomidine and fentanyl for injectable anaesthesia in dogs

dexmedetomidine and fentanyl for injectable anaesthesia in dogs. Vet. arhiv 83, 509

Wound Healing and Skin Regeneration: Present Status and Future Directions

Wound healing and skin regeneration involve intricate interactions between various cellular, molecular, and biochemical factors. This narrative review aims to provide an in-depth analysis of the present status of therapeutic strategies for wound healing and skin regeneration. The literature review was performed using the Google Scholar search engine with the help of relevant keywords. Selected publications were used to synthesize different sections of the narrative review. The quest for innovative therapeutic approaches to accelerate wound healing and enhance skin regeneration has led to remarkable advancements in recent years. The landscape of therapeutic approaches for wound healing and skin regeneration is evolving rapidly, driven by groundbreaking discoveries and interdisciplinary collaborations. From advanced wound dressings and growth factor therapies to stem cell-based interventions and gene editing techniques, the arsenal of tools at our disposal continues to expand. As researchers continue to unravel the intricate mechanisms underlying wound repair and regeneration, the potential for transformative therapies to revolutionize patient care remains immense. Through a combination of innovative technologies, personalized approaches, ethical considerations, and global accessibility, the future of wound healing holds promise for improving the lives of countless individuals worldwide. Despite significant advancements, several knowledge gaps persist in the field of wound healing and skin regeneration. Further elucidation of cellular and molecular mechanisms governing wound repair, inflammation resolution, and scar formation is warranted. Exploring the crosstalk between wound healing and the microbiome and the influence of ageing and systemic diseases will unravel new therapeutic targets and strategies. As researchers delve deeper into understanding the intricate mechanisms underlying wound repair, the development of novel therapies and their clinical translation become increasingly promising. With a multidisciplinary approach and ongoing advancements in technology, biology, and medicine, the future holds great potential for transforming the field of wound healing and skin regeneration

Prosudba učinka midazolam-ketamina s deksmedetomidinom i fentanilom za injekcijsku anesteziju u pasa.

A prospective randomized blinded study was conducted on 12 clinically healthy adult dogs of both sexes (mean weight of 18.34 ± 0.78 kg) divided into three groups (n = 4). The animals received 0.4 mg/kg midazolam and 10 μg/kg dexmedetomidine (group A), 0.4 mg/kg midazolam and 20 μg/kg dexmedetomidine (group B) and 0.4 mg/kg midazolam + 20 μg/kg dexmedetomidine + 4 μg/kg fentanyl (group C) intramuscularly, using separate syringes. Ten minutes later Ketamine was administered intravenously in all the groups. A significantly (P<0.05) shorter weak time (onset of sedation) and down time (onset of recumbency) were recorded in animals in group C as compared to the animals of groups A and B. Muscle relaxation was excellent in group C. The pedal reflex was abolished up to 30 min in groups A and B and up to 60 min in group C. Intubation was only possible in groups B and C. The anaesthetic induction dose of ketamine was minimal in group C. Standing recovery time was shortest in the animals of group C. Respiratory rate (RR) decreased significantly (P<0.05) throughout the observation period, but rectal temperature (RT) decreased significantly (P<0.05) towards the end of the observation period in all the groups. Heart rate decreased significantly (P<0.05) in the animals of group B. Mean arterial pressure (MAP) was maintained within the physiological range in all the groups. It was concluded that dexmedetomidine (10 μg/kg)-midazolam-ketamine can produce anaesthesia for about 20 min in dogs. Increasing the dose of dexmedetomidine did not enhance the duration of anaesthesia, but the further addition of fentanyl not only reduced the induction dose of ketamine but also increased the duration of anaesthesia up to 50 min. Dexmedetomidine-midazolam-fentanyl-ketamine can be used for prolonged duration of injectable anaesthesia in dogs.Poduzeto je prospektivno istraživanje na 12 slučajno odabranih klinički zdravih pasa i kuja (prosječne tjelesne mase 18,34 ± 0,78 kg) podijeljenih u tri skupine (n = 4). Životinjama skupine A bio je intramuskularno primijenjen midazolam u dozi od 0,4 mg/kg i deksmedetomidin u dozi od 10 μg/kg. Životinjama skupine B bio je i/m primijenjen midazolam u dozi od 0,4 mg/kg i deksmedetomidin 20 μg/kg, a životinje skupine C primile su i/m 0,4 mg/kg midazolama, 20 μg/kg deksmedetomidina i 4 μg/kg fentanila. Deset minuta nakon toga svim je životinjama intravenski bio ubrizgan ketamin. Značajno (P<0,05) kraće vrijeme smirivanja (nastup sedacije) i vrijeme lijeganja ustanovljeno je u životinja skupine C u usporedbi sa skupinama A i B. Opuštanje mišićja bilo je izvrsno u skupini C. Nožni refleks nestao je nakon 30 minuta u skupinama A i B, a nakon 60 minuta u skupini C. Intubacija je bila moguća samo u životinja skupine B i C. Doza ketamina potrebna za početak anestezije bila je najmanja u životinja skupine C. Vrijeme potrebno za ponovno ustajanje bilo je najkraće u životinja skupine C. Frekvencija disanja značajno se smanjila (P<0,05) u čitavom razdoblju promatranja, dok se rektalna temperatura u svih životinja značajno smanjila (P<0,05) na kraju razdoblja promatranja. Frekvencija bila znatno se smanjila (P<0,05) u životinja skupine B. Srednji arterijski tlak bio je u fiziološkim granicama u svih životinja. Može se zaključiti da kombinacija deksmedetomidin (10 μg/kg)-midazolam-ketamin može u pasa dovesti do anestezije za oko 20 minuta. Povećanje doze deksmedetomidina nije povećalo trajanje anestezije. Ipak, daljnja primjena fentanila ne samo da je smanjila početnu dozu ketamina već je povećala trajanje anestezije na 50 minuta. Deksmedetomidin-midazolam-fentanil-ketamin mogu se rabiti za produženo trajanje injekcijske anestezije u pasa

Animal Models for Wound Regeneration

&lt;p&gt;Animal models provide a valuable tool for controlled environment to investigate the complex mechanisms involved in wound healing and evaluate potential therapeutic interventions. Mouse and pig models, among others, offer insights into the cellular and molecular processes of wound healing and allow for the testing of novel treatments. This article briefs about the dimensions of animal models for wound regeneration.&lt;/p&gt

The anti-peptide relaxin antibodies for monitoring the well-being of the fetus in pregnant bitches

569-578Pregnancy management is difficult in canines and there is a lack of methodology which would allow monitoring embryonic well-being. Relaxin (Rlx) is being reported for use in pregnancy diagnosis in different species. Here, we evaluated seven anti-Rlx peptide antibodies for detection of well-being of the fetus in bitches. Peptides were synthesized using solid phase peptide synthesis chemistry and the hyper-immune sera raised in chicken against peptides. In sandwich enzyme linked immunsorbent assay (ELISA), the chicken anti-Rlx P4 as a capture and rabbit anti-prorelaxin as detection antibody, gave better results in terms of differentiating the pregnant from non-pregnant bitches. Thirty five canine serum samples (21 non-pregnant, 11 pregnant and 3 males) were screened. Among the 11 pregnant, six delivered normally and the rest of the bitches aborted a few days after the serum collection with one or two dead fetuses. Among the 11 pregnant serum samples, 4 showed the absorbance above the cut-off value set for pregnancy, which delivered healthy puppies and five bitches showed the absorbance below the set cut-off value aborted after a few days of blood collection with one or two dead fetuses. The specificity of the assay was found to be 90.47% and the sensitivity of the assay for detecting the well-being of the fetus is 82%. The serum samples collected from those bitches having problems related to normal whelping, had shown absorbance below the set cut-off value. The results of the study indicate that relaxin is a useful marker, specifically for monitoring the pregnancy and tells about the well-being of the fetus

Not Available

Not AvailablePregnancy management is difficult in canines and there is a lack of methodology which would allow monitoring embryonic well-being relaxin (Rlx) is being reported for use in pregnancy diagnosis in different species. Here, we evaluated seven anti-Rlx peptide antibodies for detection of well-being of the fetus in bitches. Peptides were synthesized using solid phase peptide synthesis chemistry and the hyper-immune sera raised in chicken against peptides. In sandwich ELISA, the chicken anti-Rlx P4 as a capture and rabbit anti-prorelaxin as detection antibody, gave better results in terms of differentiating the pregnant from non-pregnant bitches. A 35 canine serum samples (21 non-pregnant, 11 pregnant and 3 males) were screened. Among the 11 pregnant, six delivered normally and the rest of the bitches aborted a few days after the serum collection with one or two dead fetuses. Among the 11 pregnant serum samples, 4 showed the absorbance above the cutoff value set for pregnancy, which delivered healthy puppies and five bitches showed the absorbance below the set cutoff value aborted after a few days of blood collection with one or two dead fetuses. The specificity of the assay was found to be 90.47 % and the sensitivity of the assay for detecting the well-being of the fetus is 82%. The serum samples collected from those bitches having problems related to normal whelping, had shown absorbance below the set cut-off value. The results of the study indicate that relaxin is a useful marker, specifically for monitoring the pregnancy and tells about the well-being of the fetus.Not Availabl

Not Available

Not AvailableIn canines, a physical examination and ultrasound technique remain the most common methods of pregnancy diagnosis. The rapid detection of pregnancy is important in canines. There are several reports of peptide hormone relaxin to be exclusively produced in pregnant bitches and not by non-pregnant or pseudo pregnant bitches. We present here the expression of canine relaxin in Pichaia pastoris GS115 host. The cDNA prepared using canine placental total RNA was used to amplify 432 bp relaxin gene, which was subsequently cloned in pPICZ-alphaA vector and followed by transformation into Pichia pastoris GS115 strain by electroporation. The zeocin resistant recombinant clones were confirmed by colony PCR and sequencing. One of the positive clones was selected for protein expression in culture medium. The supernatant was taken and precipitated using 20% TCA in acetone. The recombinant relaxin precursor protein showed a mol wt of 24 kDa, which is higher than the expected 19 kDa and that could be due to glycosylation in the Pichia pastoris. The 24 kDa recombinant protein was detected up to 48 h of post-induction. This is the first report describing the expression of recombinant canine relaxin in Pichia pastoris. The polyclonal antiserum against canine relaxin would be useful for detection of pregnancy in bitches and also in assessing the prognosis of canine mammary tumorsNot Availabl